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Literature summary extracted from

  • Dube, E.; Shareck, F.; Hurtubise, Y.; Daneault, C.; Beauregard, M.
    Homologous cloning, expression, and characterisation of a laccase from Streptomyces coelicolor and enzymatic decolourisation of an indigo dye (2008), Appl. Microbiol. Biotechnol., 79, 597-603.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.10.3.2 additional information the enzyme may be used in a variety of biotechnological applications, including textile dye bleaching, pulp bleaching, bioremediation, polymer synthesis and biosensors Streptomyces coelicolor

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.10.3.2 recombinant expression in Streptomyces lividans, the enzyme is secreted Streptomyces coelicolor

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 additional information no inhibition by EDTA at 1-10 mM Streptomyces coelicolor
1.10.3.2 NaN3 22% inhibition at 10 mM, no inhibition by 1 mM NaN3 Streptomyces coelicolor

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 Cu2+ a copper-containing enzyme Streptomyces coelicolor

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Streptomyces coelicolor
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2 extracellular recombinant enzyme expressed in Streptomyces lividans to homogeneity Streptomyces coelicolor

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.2 8
-
 purified recombinant enzyme, with substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid at pH 4.0 Streptomyces coelicolor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Streptomyces coelicolor ?
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2
-
 Streptomyces coelicolor ?
-
 ?
1.10.3.2 syringaldazine + O2 the enzyme is able to rapidly decolourize the dye Indigo carmine in the presence of syringaldehyde as a redox mediator, at pH 9.0 and 45°C Streptomyces coelicolor ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.10.3.2 SLAC
-
 Streptomyces coelicolor

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 60
-
 at pH 4.0 Streptomyces coelicolor

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.10.3.2 30 70
-
 Streptomyces coelicolor

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 70
-
 purified recombinant enzyme, 90% remaining activity after 25 min, 50% remaining actiivty after 110 min Streptomyces coelicolor

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 4
-
 assay at, substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid Streptomyces coelicolor
1.10.3.2 9
-
 assay at, substrate 2,6-dimethoxyphenol Streptomyces coelicolor

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.10.3.2 4 9 activity range, dependent on the substrate Streptomyces coelicolor

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.10.3.2 3 9 purified recombinant enzyme, 48 h, 30°C, completely stable Streptomyces coelicolor