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Literature summary extracted from
- Cloning, characterization and functional expression of an alkalitolerant type C feruloyl esterase from Fusarium oxysporum (2008), Appl. Microbiol. Biotechnol., 79, 245-254.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.1.1.73 | food industry | ferulic acid released from plant cell wall by the action of FAEs is an effective natural antioxidant with potential applications in the pharmaceutical and food industries | Fusarium oxysporum |
| 3.1.1.73 | paper production | FAEs could be used in pulp and paper processing and as animal feed additives to facilitate nutrient assimilation | Fusarium oxysporum |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.1.73 | expression in Pichia pastoris X-33 | Fusarium oxysporum |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.73 | 8.2 | - |
methyl sinapate | - |
Fusarium oxysporum |  |
| 3.1.1.73 | 100.5 | - |
n-propyl ferulate | - |
Fusarium oxysporum | |
| 3.1.1.73 | 104.7 | - |
iso-butyl ferulate | - |
Fusarium oxysporum | |
| 3.1.1.73 | 104.8 | - |
iso-propyl ferulate | - |
Fusarium oxysporum | |
| 3.1.1.73 | 118.1 | - |
ethyl ferulate | - |
Fusarium oxysporum | |
| 3.1.1.73 | 120.4 | - |
methyl ferulate | - |
Fusarium oxysporum | |
| 3.1.1.73 | 139.8 | - |
2-butyl ferulate | - |
Fusarium oxysporum | |
| 3.1.1.73 | 140.6 | - |
4-nitrophenyl 2-O-trans-feruloyl-alpha-L-arabinofuranoside | - |
Fusarium oxysporum | |
| 3.1.1.73 | 140.7 | - |
methyl caffeate | - |
Fusarium oxysporum | |
| 3.1.1.73 | 222.9 | - |
methyl p-coumarate | - |
Fusarium oxysporum | |
| 3.1.1.73 | 629.1 | - |
n-butyl ferulate | - |
Fusarium oxysporum | |
| 3.1.1.73 | 3332 | - |
4-nitrophenyl 5-O-trans-feruloyl-alpha-L-arabinofuranoside | - |
Fusarium oxysporum | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.1.73 | extracellular | - |
Fusarium oxysporum | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.73 | 62000 | - |
SDS-PAGE | Fusarium oxysporum |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.73 | Fusarium oxysporum | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.73 | ultrafiltration, anion-exchange chromatography | Fusarium oxysporum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.73 | 2-butyl ferulate + H2O | - |
Fusarium oxysporum | ferulic acid + 2-butanol | - |
? | |
| 3.1.1.73 | 4-nitrophenyl 2-O-trans-feruloyl-alpha-L-arabinofuranoside + H2O | The comparison of kcat/KM ratio reveals that the enzyme hydrolyzes C-5 position slightly faster and more efficiently than C-2 position, demonstrating the broad substrate specificity of the esterase | Fusarium oxysporum | ferulic acid + 4-nitrophenyl-alpha-L-arabinofuranoside | - |
? | |
| 3.1.1.73 | 4-nitrophenyl 5-O-trans-feruloyl-alpha-L-arabinofuranoside + H2O | The comparison of kcat/KM ratio reveals that the enzyme hydrolyzes C-5 position slightly faster and more efficiently than C-2 position, demonstrating the broad substrate specificity of the esterase | Fusarium oxysporum | ferulic acid + 4-nitrophenyl alpha-L-arabinofuranoside | - |
? | |
| 3.1.1.73 | ethyl ferulate + H2O | - |
Fusarium oxysporum | ferulic acid + ethanol | - |
? | |
| 3.1.1.73 | iso-butyl ferulate + H2O | - |
Fusarium oxysporum | ferulic acid + iso-butanol | - |
? | |
| 3.1.1.73 | iso-propyl ferulate + H2O | - |
Fusarium oxysporum | ferulic acid + iso-propanol | - |
? | |
| 3.1.1.73 | methyl caffeate + H2O | - |
Fusarium oxysporum | methanol + caffeic acid | - |
? | |
| 3.1.1.73 | methyl ferulate + H2O | - |
Fusarium oxysporum | methanol + ferulic acid | - |
? | |
| 3.1.1.73 | methyl p-coumarate + H2O | - |
Fusarium oxysporum | methanol + p-coumaric acid | - |
? | |
| 3.1.1.73 | methyl sinapate + H2O | - |
Fusarium oxysporum | methanol + sinapic acid | - |
? | |
| 3.1.1.73 | additional information | The substrate specificity profiling reveals that the enzyme is a type C FAE showing broad hydrolytic activity against the four methyl esters of hydroxycinnamic acids and strong preference for the hydrolysis of n-propyl ferulate. | Fusarium oxysporum | ? | - |
? | |
| 3.1.1.73 | n-butyl ferulate + H2O | - |
Fusarium oxysporum | ferulic acid + n-butanol | - |
? | |
| 3.1.1.73 | n-propyl ferulate + H2O | - |
Fusarium oxysporum | ferulic acid + n-propanol | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.1.73 | Cinnamoyl esterase | - |
Fusarium oxysporum |
| 3.1.1.73 | FAE | - |
Fusarium oxysporum |
| 3.1.1.73 | feruloyl esterase | - |
Fusarium oxysporum |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.73 | 65 | - |
- |
Fusarium oxysporum |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.73 | additional information | - |
the enzyme retained 85% of its activity at 70°C while obove that temperature the catalytic ability is lost quickly | Fusarium oxysporum |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.73 | 0.12 | - |
methyl ferulate | - |
Fusarium oxysporum | |
| 3.1.1.73 | 0.16 | - |
methyl caffeate | - |
Fusarium oxysporum | |
| 3.1.1.73 | 0.18 | - |
n-propyl ferulate | - |
Fusarium oxysporum | |
| 3.1.1.73 | 0.21 | - |
methyl p-coumarate | - |
Fusarium oxysporum | |
| 3.1.1.73 | 0.26 | - |
iso-propyl ferulate | - |
Fusarium oxysporum | |
| 3.1.1.73 | 0.46 | - |
ethyl ferulate | - |
Fusarium oxysporum | |
| 3.1.1.73 | 0.5 | - |
methyl sinapate | - |
Fusarium oxysporum | |
| 3.1.1.73 | 0.95 | - |
iso-butyl ferulate | - |
Fusarium oxysporum | |
| 3.1.1.73 | 1.24 | - |
2-butyl ferulate | - |
Fusarium oxysporum | |
| 3.1.1.73 | 1.55 | - |
4-nitrophenyl 5-O-trans-feruloyl-alpha-L-arabinofuranoside | - |
Fusarium oxysporum | |
| 3.1.1.73 | 1.76 | - |
4-nitrophenyl 2-O-trans-feruloyl-alpha-L-arabinofuranoside | - |
Fusarium oxysporum | |
| 3.1.1.73 | 14.05 | - |
n-butyl ferulate | - |
Fusarium oxysporum | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.73 | 6 | - |
- |
Fusarium oxysporum |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.73 | 4 | 11 | - |
Fusarium oxysporum |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.73 | additional information | - |
the esterase shows broad pH stability making it an important candidate for alkaline applications such as pulp treatment in the paper industry | Fusarium oxysporum |
| 3.1.1.73 | 4 | 11 | retaining more than 80% of the initial activity after 24 h | Fusarium oxysporum |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.1.1.73 | Fusarium oxysporum | IEF-PAGE | - |
6.8 |
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