EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.2.1.6 | Q487A | mutation diminishes decarboxylase activity but maintains the synthase activity | Bacillus subtilis |
2.2.1.6 | Q487A | wild-type additionally catalyzes the decarboxylation of 2-oxoisovalerate. Mutation diminishes only the decarboxylase activity but maintains the acetolactate synthase activity | Bacillus subtilis |
2.2.1.6 | Q487G | mutation diminishes decarboxylase activity but maintains the synthase activity | Bacillus subtilis |
2.2.1.6 | Q487G | wild-type additionally catalyzes the decarboxylation of 2-oxoisovalerate. Mutation diminishes only the decarboxylase activity but maintains the acetolactate synthase activity | Bacillus subtilis |
2.2.1.6 | Q487I | complete loss of synthase activity | Bacillus subtilis |
2.2.1.6 | Q487I | loss of acetolactate synthase activity, decrease in decarboxylase activity | Bacillus subtilis |
2.2.1.6 | Q487L | complete loss of synthase activity | Bacillus subtilis |
2.2.1.6 | Q487L | loss of acetolactate synthase activity, decrease in decarboxylase activity | Bacillus subtilis |
2.2.1.6 | Q487S | mutation diminishes decarboxylase activity but maintains the synthase activity | Bacillus subtilis |
2.2.1.6 | Q487S | wild-type additionally catalyzes the decarboxylation of 2-oxoisovalerate. Mutation diminishes only the decarboxylase activity but maintains the acetolactate synthase activity | Bacillus subtilis |
4.1.1.72 | Q487A | mutation diminishes the decarboxylase activity but maintains the acetolactate synthase activity | Bacillus subtilis |
4.1.1.72 | Q487G | mutation diminishes only the decarboxylase activity but maintains the acetolactate synthase activity | Bacillus subtilis |
4.1.1.72 | Q487I | loss of acetolactate synthase activity, decrease in decarboxylase activity | Bacillus subtilis |
4.1.1.72 | Q487L | loss of acetolactate synthase activity, decrease in decarboxylase activity | Bacillus subtilis |
4.1.1.72 | Q487S | mutation diminishes only the decarboxylase activity but maintains the acetolactate synthase activity | Bacillus subtilis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.2.1.6 | 1.1 | - |
pyruvate | mutant Q487S, pH 7.0, 37°C | Bacillus subtilis | |
2.2.1.6 | 1.6 | - |
pyruvate | mutant Q487G, pH 7.0, 37°C | Bacillus subtilis | |
2.2.1.6 | 8.7 | - |
pyruvate | mutant Q487A, pH 7.0, 37°C | Bacillus subtilis | |
2.2.1.6 | 13.6 | - |
pyruvate | wild-type, pH 7.0, 37°C | Bacillus subtilis | |
4.1.1.72 | 154 | - |
2-oxoisovalerate | mutant Q487S, pH 7.0, 37°C | Bacillus subtilis | |
4.1.1.72 | 175 | - |
2-oxoisovalerate | mutant Q487G, pH 7.0, 37°C | Bacillus subtilis | |
4.1.1.72 | 186 | - |
2-oxoisovalerate | mutant Q487A, pH 7.0, 37°C | Bacillus subtilis | |
4.1.1.72 | 300 | - |
2-oxoisovalerate | wild-type, pH 7.0, 37°C | Bacillus subtilis | |
4.1.1.72 | 323 | - |
2-oxoisovalerate | mutant Q487I, pH 7.0, 37°C | Bacillus subtilis | |
4.1.1.72 | 342 | - |
2-oxoisovalerate | mutant Q487L, pH 7.0, 37°C | Bacillus subtilis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.2.1.6 | Bacillus subtilis | - |
- |
- |
2.2.1.6 | Bacillus subtilis | Q04789 | - |
- |
2.2.1.6 | Bacillus subtilis 168 | Q04789 | - |
- |
4.1.1.72 | Bacillus subtilis | Q04789 | cf. EC 2.2.1.6 | - |
4.1.1.72 | Bacillus subtilis 168 | Q04789 | cf. EC 2.2.1.6 | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.2.1.6 | 2 pyruvate | - |
Bacillus subtilis | 2-acetolactate + CO2 | - |
? | |
2.2.1.6 | 2 pyruvate | - |
Bacillus subtilis 168 | 2-acetolactate + CO2 | - |
? | |
2.2.1.6 | additional information | enzyme additionallly displays 2-ketoisovalerate decarboxylase activity | Bacillus subtilis | ? | - |
? | |
2.2.1.6 | additional information | acetolactate synthase AlsS is able to catalyze the decarboxylation of 2-oxoisovalerate both in vivo and in vitro | Bacillus subtilis | ? | - |
? | |
2.2.1.6 | additional information | acetolactate synthase AlsS is able to catalyze the decarboxylation of 2-oxoisovalerate both in vivo and in vitro | Bacillus subtilis 168 | ? | - |
? | |
2.2.1.6 | pyruvate | - |
Bacillus subtilis | (S)-2-acetolactate + CO2 | - |
? | |
4.1.1.72 | 2-oxoisovalerate | - |
Bacillus subtilis | isobutanal + CO2 | - |
? | |
4.1.1.72 | 2-oxoisovalerate | - |
Bacillus subtilis 168 | isobutanal + CO2 | - |
? | |
4.1.1.72 | additional information | acetolactate synthase AlsS, EC 2.2.1.6, is able to catalyze the decarboxylation of 2-oxoisovalerate both in vivo and in vitro | Bacillus subtilis | ? | - |
? | |
4.1.1.72 | additional information | acetolactate synthase AlsS, EC 2.2.1.6, is able to catalyze the decarboxylation of 2-oxoisovalerate both in vivo and in vitro | Bacillus subtilis 168 | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.2.1.6 | AlsS | - |
Bacillus subtilis |
4.1.1.72 | AlsS | - |
Bacillus subtilis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.2.1.6 | 11 | - |
pyruvate | mutant Q487G, pH 7.0, 37°C | Bacillus subtilis | |
2.2.1.6 | 11 | - |
pyruvate | mutant Q487S, pH 7.0, 37°C | Bacillus subtilis | |
2.2.1.6 | 58 | - |
pyruvate | mutant Q487A, pH 7.0, 37°C | Bacillus subtilis | |
2.2.1.6 | 121 | - |
pyruvate | wild-type, pH 7.0, 37°C | Bacillus subtilis | |
4.1.1.72 | 0.8 | - |
2-oxoisovalerate | mutant Q487G, pH 7.0, 37°C | Bacillus subtilis | |
4.1.1.72 | 0.8 | - |
2-oxoisovalerate | mutant Q487S, pH 7.0, 37°C | Bacillus subtilis | |
4.1.1.72 | 1.1 | - |
2-oxoisovalerate | mutant Q487A, pH 7.0, 37°C | Bacillus subtilis | |
4.1.1.72 | 4.8 | - |
2-oxoisovalerate | mutant Q487I, pH 7.0, 37°C | Bacillus subtilis | |
4.1.1.72 | 5.4 | - |
2-oxoisovalerate | mutant Q487L, pH 7.0, 37°C | Bacillus subtilis | |
4.1.1.72 | 8.9 | - |
2-oxoisovalerate | wild-type, pH 7.0, 37°C | Bacillus subtilis |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.2.1.6 | 6.7 | - |
pyruvate | mutant Q487A, pH 7.0, 37°C | Bacillus subtilis | |
2.2.1.6 | 6.9 | - |
pyruvate | mutant Q487G, pH 7.0, 37°C | Bacillus subtilis | |
2.2.1.6 | 8.9 | - |
pyruvate | wild-type, pH 7.0, 37°C | Bacillus subtilis | |
2.2.1.6 | 10 | - |
pyruvate | mutant Q487S, pH 7.0, 37°C | Bacillus subtilis | |
4.1.1.72 | 0.005 | - |
2-oxoisovalerate | mutant Q487G, pH 7.0, 37°C | Bacillus subtilis | |
4.1.1.72 | 0.005 | - |
2-oxoisovalerate | mutant Q487S, pH 7.0, 37°C | Bacillus subtilis | |
4.1.1.72 | 0.006 | - |
2-oxoisovalerate | mutant Q487A, pH 7.0, 37°C | Bacillus subtilis | |
4.1.1.72 | 0.01 | - |
2-oxoisovalerate | mutant Q487I, pH 7.0, 37°C | Bacillus subtilis | |
4.1.1.72 | 0.02 | - |
2-oxoisovalerate | mutant Q487L, pH 7.0, 37°C | Bacillus subtilis | |
4.1.1.72 | 0.03 | - |
2-oxoisovalerate | wild-type, pH 7.0, 37°C | Bacillus subtilis |