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Literature summary extracted from

  • Atsumi, S.; Li, Z.; Liao, J.C.
    Acetolactate synthase from Bacillus subtilis serves as a 2-ketoisovalerate decarboxylase for isobutanol biosynthesis in Escherichia coli (2009), Appl. Environ. Microbiol., 75, 6306-6311.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
2.2.1.6 Q487A mutation diminishes decarboxylase activity but maintains the synthase activity Bacillus subtilis
2.2.1.6 Q487A wild-type additionally catalyzes the decarboxylation of 2-oxoisovalerate. Mutation diminishes only the decarboxylase activity but maintains the acetolactate synthase activity Bacillus subtilis
2.2.1.6 Q487G mutation diminishes decarboxylase activity but maintains the synthase activity Bacillus subtilis
2.2.1.6 Q487G wild-type additionally catalyzes the decarboxylation of 2-oxoisovalerate. Mutation diminishes only the decarboxylase activity but maintains the acetolactate synthase activity Bacillus subtilis
2.2.1.6 Q487I complete loss of synthase activity Bacillus subtilis
2.2.1.6 Q487I loss of acetolactate synthase activity, decrease in decarboxylase activity Bacillus subtilis
2.2.1.6 Q487L complete loss of synthase activity Bacillus subtilis
2.2.1.6 Q487L loss of acetolactate synthase activity, decrease in decarboxylase activity Bacillus subtilis
2.2.1.6 Q487S mutation diminishes decarboxylase activity but maintains the synthase activity Bacillus subtilis
2.2.1.6 Q487S wild-type additionally catalyzes the decarboxylation of 2-oxoisovalerate. Mutation diminishes only the decarboxylase activity but maintains the acetolactate synthase activity Bacillus subtilis
4.1.1.72 Q487A mutation diminishes the decarboxylase activity but maintains the acetolactate synthase activity Bacillus subtilis
4.1.1.72 Q487G mutation diminishes only the decarboxylase activity but maintains the acetolactate synthase activity Bacillus subtilis
4.1.1.72 Q487I loss of acetolactate synthase activity, decrease in decarboxylase activity Bacillus subtilis
4.1.1.72 Q487L loss of acetolactate synthase activity, decrease in decarboxylase activity Bacillus subtilis
4.1.1.72 Q487S mutation diminishes only the decarboxylase activity but maintains the acetolactate synthase activity Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2.1.6 1.1
-
 pyruvate mutant Q487S, pH 7.0, 37°C Bacillus subtilis
2.2.1.6 1.6
-
 pyruvate mutant Q487G, pH 7.0, 37°C Bacillus subtilis
2.2.1.6 8.7
-
 pyruvate mutant Q487A, pH 7.0, 37°C Bacillus subtilis
2.2.1.6 13.6
-
 pyruvate wild-type, pH 7.0, 37°C Bacillus subtilis
4.1.1.72 154
-
 2-oxoisovalerate mutant Q487S, pH 7.0, 37°C Bacillus subtilis
4.1.1.72 175
-
 2-oxoisovalerate mutant Q487G, pH 7.0, 37°C Bacillus subtilis
4.1.1.72 186
-
 2-oxoisovalerate mutant Q487A, pH 7.0, 37°C Bacillus subtilis
4.1.1.72 300
-
 2-oxoisovalerate wild-type, pH 7.0, 37°C Bacillus subtilis
4.1.1.72 323
-
 2-oxoisovalerate mutant Q487I, pH 7.0, 37°C Bacillus subtilis
4.1.1.72 342
-
 2-oxoisovalerate mutant Q487L, pH 7.0, 37°C Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.6 Bacillus subtilis
-
-
-
2.2.1.6 Bacillus subtilis Q04789
-
-
2.2.1.6 Bacillus subtilis 168 Q04789
-
-
4.1.1.72 Bacillus subtilis Q04789 cf. EC 2.2.1.6
-
4.1.1.72 Bacillus subtilis 168 Q04789 cf. EC 2.2.1.6
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.6 2 pyruvate
-
 Bacillus subtilis 2-acetolactate + CO2
-
 ?
2.2.1.6 2 pyruvate
-
 Bacillus subtilis 168 2-acetolactate + CO2
-
 ?
2.2.1.6 additional information enzyme additionallly displays 2-ketoisovalerate decarboxylase activity Bacillus subtilis ?
-
 ?
2.2.1.6 additional information acetolactate synthase AlsS is able to catalyze the decarboxylation of 2-oxoisovalerate both in vivo and in vitro Bacillus subtilis ?
-
 ?
2.2.1.6 additional information acetolactate synthase AlsS is able to catalyze the decarboxylation of 2-oxoisovalerate both in vivo and in vitro Bacillus subtilis 168 ?
-
 ?
2.2.1.6 pyruvate
-
 Bacillus subtilis (S)-2-acetolactate + CO2
-
 ?
4.1.1.72 2-oxoisovalerate
-
 Bacillus subtilis isobutanal + CO2
-
 ?
4.1.1.72 2-oxoisovalerate
-
 Bacillus subtilis 168 isobutanal + CO2
-
 ?
4.1.1.72 additional information acetolactate synthase AlsS, EC 2.2.1.6, is able to catalyze the decarboxylation of 2-oxoisovalerate both in vivo and in vitro Bacillus subtilis ?
-
 ?
4.1.1.72 additional information acetolactate synthase AlsS, EC 2.2.1.6, is able to catalyze the decarboxylation of 2-oxoisovalerate both in vivo and in vitro Bacillus subtilis 168 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.2.1.6 AlsS
-
 Bacillus subtilis
4.1.1.72 AlsS
-
 Bacillus subtilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.2.1.6 11
-
 pyruvate mutant Q487G, pH 7.0, 37°C Bacillus subtilis
2.2.1.6 11
-
 pyruvate mutant Q487S, pH 7.0, 37°C Bacillus subtilis
2.2.1.6 58
-
 pyruvate mutant Q487A, pH 7.0, 37°C Bacillus subtilis
2.2.1.6 121
-
 pyruvate wild-type, pH 7.0, 37°C Bacillus subtilis
4.1.1.72 0.8
-
 2-oxoisovalerate mutant Q487G, pH 7.0, 37°C Bacillus subtilis
4.1.1.72 0.8
-
 2-oxoisovalerate mutant Q487S, pH 7.0, 37°C Bacillus subtilis
4.1.1.72 1.1
-
 2-oxoisovalerate mutant Q487A, pH 7.0, 37°C Bacillus subtilis
4.1.1.72 4.8
-
 2-oxoisovalerate mutant Q487I, pH 7.0, 37°C Bacillus subtilis
4.1.1.72 5.4
-
 2-oxoisovalerate mutant Q487L, pH 7.0, 37°C Bacillus subtilis
4.1.1.72 8.9
-
 2-oxoisovalerate wild-type, pH 7.0, 37°C Bacillus subtilis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.2.1.6 6.7
-
 pyruvate mutant Q487A, pH 7.0, 37°C Bacillus subtilis
2.2.1.6 6.9
-
 pyruvate mutant Q487G, pH 7.0, 37°C Bacillus subtilis
2.2.1.6 8.9
-
 pyruvate wild-type, pH 7.0, 37°C Bacillus subtilis
2.2.1.6 10
-
 pyruvate mutant Q487S, pH 7.0, 37°C Bacillus subtilis
4.1.1.72 0.005
-
 2-oxoisovalerate mutant Q487G, pH 7.0, 37°C Bacillus subtilis
4.1.1.72 0.005
-
 2-oxoisovalerate mutant Q487S, pH 7.0, 37°C Bacillus subtilis
4.1.1.72 0.006
-
 2-oxoisovalerate mutant Q487A, pH 7.0, 37°C Bacillus subtilis
4.1.1.72 0.01
-
 2-oxoisovalerate mutant Q487I, pH 7.0, 37°C Bacillus subtilis
4.1.1.72 0.02
-
 2-oxoisovalerate mutant Q487L, pH 7.0, 37°C Bacillus subtilis
4.1.1.72 0.03
-
 2-oxoisovalerate wild-type, pH 7.0, 37°C Bacillus subtilis