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Literature summary extracted from

  • Martinez-Gomez, A.I.; Martinez-Rodriguez, S.; Pozo-Dengra, J.; Tessaro, D.; Servi, S.; Clemente-Jimenez, J.M.; Rodriguez-Vico, F.; Las Heras-Vazquez, F.J.
    Potential application of N-carbamoyl-beta-alanine amidohydrolase from Agrobacterium tumefaciens C58 for beta-amino acid production (2009), Appl. Environ. Microbiol., 75, 514-520.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.6
-
 Agrobacterium tumefaciens

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.6 R291E R291 is the key residue for recognition of the substrate carboxyl group Agrobacterium tumefaciens
3.5.1.6 R291K R291 is the key residue for recognition of the substrate carboxyl group Agrobacterium tumefaciens
3.5.1.6 R291Q R291 is the key residue for recognition of the substrate carboxyl group Agrobacterium tumefaciens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.6 8-Hydroxyquinoline-5-sulfonic acid
-
 Agrobacterium tumefaciens
3.5.1.6 Cu2+ strong inhibition Agrobacterium tumefaciens
3.5.1.6 EDTA
-
 Agrobacterium tumefaciens
3.5.1.6 Hg2+ slight inhibition Agrobacterium tumefaciens
3.5.1.6 Zn2+ slight inhibition Agrobacterium tumefaciens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.6 0.07
-
 2-amino-3-ureidopropionic acid
-
 Agrobacterium tumefaciens
3.5.1.6 2.14
-
 3-ureidopropionic acid
-
 Agrobacterium tumefaciens
3.5.1.6 3.44
-
 3-methyl-3-ureidopropionic acid
-
 Agrobacterium tumefaciens
3.5.1.6 6.59
-
 2-methyl-3-ureidopropionic acid
-
 Agrobacterium tumefaciens
3.5.1.6 10.58
-
 2-ureidoethanesulfonic acid
-
 Agrobacterium tumefaciens
3.5.1.6 20.15
-
 2-ureidoethane phosphonic acid
-
 Agrobacterium tumefaciens
3.5.1.6 84.37
-
 2-phenyl-3-ureidopropionic acid
-
 Agrobacterium tumefaciens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.6 Co2+
-
 Agrobacterium tumefaciens
3.5.1.6 Mn2+
-
 Agrobacterium tumefaciens
3.5.1.6 Ni2+
-
 Agrobacterium tumefaciens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.6 45000
-
 2 * 45000, SDS-PAGE Agrobacterium tumefaciens
3.5.1.6 90000
-
 gel filtration Agrobacterium tumefaciens

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.6 Agrobacterium tumefaciens
-
-
-
3.5.1.6 Agrobacterium tumefaciens C58 / ATCC 33970
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.6
-
 Agrobacterium tumefaciens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.6 2-amino-3-ureidopropionic acid + H2O
-
 Agrobacterium tumefaciens 3-aminoalanine + CO2 + NH3
-
 ?
3.5.1.6 2-amino-3-ureidopropionic acid + H2O
-
 Agrobacterium tumefaciens C58 / ATCC 33970 3-aminoalanine + CO2 + NH3
-
 ?
3.5.1.6 2-methyl-3-ureidopropionic acid + H2O
-
 Agrobacterium tumefaciens alpha-methyl-beta-alanine + CO2 + NH3
-
 ?
3.5.1.6 2-methyl-3-ureidopropionic acid + H2O
-
 Agrobacterium tumefaciens C58 / ATCC 33970 alpha-methyl-beta-alanine + CO2 + NH3
-
 ?
3.5.1.6 2-phenyl-3-ureidopropionic acid + H2O
-
 Agrobacterium tumefaciens 3-amino-2-phenylpropanoic acid + CO2 + NH3
-
 ?
3.5.1.6 2-phenyl-3-ureidopropionic acid + H2O
-
 Agrobacterium tumefaciens C58 / ATCC 33970 3-amino-2-phenylpropanoic acid + CO2 + NH3
-
 ?
3.5.1.6 2-ureidoethane phosphonic acid + H2O
-
 Agrobacterium tumefaciens ciliatine + CO2 + NH3
-
 ?
3.5.1.6 2-ureidoethane phosphonic acid + H2O
-
 Agrobacterium tumefaciens C58 / ATCC 33970 ciliatine + CO2 + NH3
-
 ?
3.5.1.6 2-ureidoethanesulfonic acid + H2O
-
 Agrobacterium tumefaciens taurine + CO2 + NH3
-
 ?
3.5.1.6 3-methyl-3-ureidopropionic acid + H2O
-
 Agrobacterium tumefaciens beta-homoalanine + CO2 + NH3
-
 ?
3.5.1.6 3-ureidopropionic acid + H2O
-
 Agrobacterium tumefaciens beta-alanine + CO2 + NH3
-
 ?
3.5.1.6 N-carbamoyl-beta-alanine + H2O
-
 Agrobacterium tumefaciens beta-alanine + CO2 + NH3
-
 ?
3.5.1.6 N-carbamoyl-beta-alanine + H2O
-
 Agrobacterium tumefaciens C58 / ATCC 33970 beta-alanine + CO2 + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.6 homodimer 2 * 45000, SDS-PAGE Agrobacterium tumefaciens

Synonyms

EC Number Synonyms Comment Organism
3.5.1.6 N-carbamoyl-beta-alanine amidohydrolase
-
 Agrobacterium tumefaciens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.6 30
-
-
 Agrobacterium tumefaciens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.6 45
-
 after 60 min 20% activity Agrobacterium tumefaciens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.6 0.13
-
 2-phenyl-3-ureidopropionic acid
-
 Agrobacterium tumefaciens
3.5.1.6 0.15
-
 2-amino-3-ureidopropionic acid
-
 Agrobacterium tumefaciens
3.5.1.6 0.2
-
 3-methyl-3-ureidopropionic acid
-
 Agrobacterium tumefaciens
3.5.1.6 0.23
-
 2-ureidoethane phosphonic acid
-
 Agrobacterium tumefaciens
3.5.1.6 5.84
-
 2-ureidoethanesulfonic acid
-
 Agrobacterium tumefaciens
3.5.1.6 24.39
-
 2-methyl-3-ureidopropionic acid
-
 Agrobacterium tumefaciens
3.5.1.6 25.71
-
 3-ureidopropionic acid
-
 Agrobacterium tumefaciens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.6 8
-
-
 Agrobacterium tumefaciens