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Literature summary extracted from

  • Saichana, I.; Moonmangmee, D.; Adachi, O.; Matsushita, K.; Toyama, H.
    Screening of thermotolerant Gluconobacter strains for production of 5-keto-D-gluconic acid and disruption of flavin adenine dinucleotide-containing D-gluconate dehydrogenase (2009), Appl. Environ. Microbiol., 75, 4240-4247.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.99.3 into the pGEM-T easy vector for sequencing Gluconobacter sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.99.3 membrane
-
 Gluconobacter sp. 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.99.22 Ca2+
-
 Gluconobacter sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.99.3 D-gluconate + FAD Gluconobacter sp.
-
 2-dehydro-D-gluconate + FADH2
-
 ?
1.1.99.3 D-gluconate + FAD Gluconobacter sp. THE42
-
 2-dehydro-D-gluconate + FADH2
-
 ?
1.1.99.22 additional information Gluconobacter sp. broad substrate specificity but high regio- and stereospecificity ?
-
 ?
1.1.99.22 additional information Gluconobacter sp. THE42 broad substrate specificity but high regio- and stereospecificity ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.3 Gluconobacter sp.
-
-
-
1.1.99.22 Gluconobacter sp.
-
-
-
1.1.99.22 Gluconobacter sp. THE42
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.3 D-gluconate + FAD
-
 Gluconobacter sp. 2-dehydro-D-gluconate + FADH2
-
 ?
1.1.99.3 D-gluconate + FAD
-
 Gluconobacter sp. THE42 2-dehydro-D-gluconate + FADH2
-
 ?
1.1.99.22 additional information broad substrate specificity but high regio- and stereospecificity Gluconobacter sp. ?
-
 ?
1.1.99.22 additional information broad substrate specificity but high regio- and stereospecificity Gluconobacter sp. THE42 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.99.3 D-gluconate dehydrogenase
-
 Gluconobacter sp.
1.1.99.3 GADH
-
 Gluconobacter sp.
1.1.99.22 5-keto-D-gluconate-producing enzyme
-
 Gluconobacter sp.
1.1.99.22 glycerol dehydrogenase
-
 Gluconobacter sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.99.3 25
-
 activity assay Gluconobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.99.3 5
-
 activity assay Gluconobacter sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.99.3 FAD
-
 Gluconobacter sp.
1.1.99.3 heme
-
 Gluconobacter sp.
1.1.99.22 pyrroloquinoline quinone
-
 Gluconobacter sp.