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Literature summary extracted from

  • Zhao, Q.; Hou, Y.; Gong, G.H.; Yu, M.A.; Jiang, L.; Liao, F.
    Characterization of alcohol dehydrogenase from permeabilized brewers yeast cells immobilized on the derived attapulgite nanofibers (2009), Appl. Biochem. Biotechnol., 160, 2287-2299.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.1 Glutaraldehyde treating with 0.5% glutaraldehyde solution, the activity of the immobilized enzyme is at maximum Saccharomyces cerevisiae

General Stability

EC Number General Stability Organism
1.1.1.1 ADH immobilized on derived attapulgite nanofibers via glutaraldehyde covalent binding retains higher activity over wider ranges of pH and temperature than those of the free enzyme. After shaking at 125 rpm at 35°C for 32 h, a rapid loss in activity is observed, and almost complete activity of immobilized enzyme is lost in 52 h. The activity of immobilized ADH decreases to 80% of its initial value after four cycles of operation and afterwards gradually decreases with every reuse, but it retains 42% activity after eight cycles for bioreduction of ethyl 3-oxobutyrate. Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.1 Glutaraldehyde 71% relative activity in the presence of 10 mM glutaraldehyde Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.1 Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.1 ethanol + NAD+
-
 Saccharomyces cerevisiae acetaldehyde + NADH + H+
-
 ?
1.1.1.1 ethyl 3-oxobutyrate + NADH + H+
-
 Saccharomyces cerevisiae ethyl (S)-3-hydroxybutyrate + NAD+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.1 ADH
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.1 25
-
 free enzyme, at 25°C Saccharomyces cerevisiae
1.1.1.1 35
-
 immobilized enzyme Saccharomyces cerevisiae

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.1 15 50
-
 Saccharomyces cerevisiae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.1 35 60 58% of the original activity is retained after incubation of the immobilized enzyme at 35°C for 32 h, free enzyme loses 68% activity over a 60 min incubation at 60°C, whereas immobilized ADH retains 44% over a 60 min incubation at 60°C Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.1 7
-
 free enzyme, at 25°C Saccharomyces cerevisiae
1.1.1.1 7.5
-
 immobilized enzyme, at 25°C Saccharomyces cerevisiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.1 5 9
-
 Saccharomyces cerevisiae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.1 6 8 at pH 6.0 and 8.0, the activity of free ADH decreases dramatically during the incubation, and 90 min later most of the activity is lost, the immobilized form retains 81% of activity at pH 8.0 Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.1 NAD+
-
 Saccharomyces cerevisiae