EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.5.2.6 | S287N | the AmpC variants AmpC-A and AmpC-B2 harboring the S287N substitution are obtained by mutagenesis from cephalosporinases representative of the phylogenetic groups A and B2 of Escherichia coli. Their biochemical characterization reveals that the S287N replacement leads to an important increase in the catalytic efficiency toward extended-spectrum cephalosporins in the AmpC beta-lactamase of group A only | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.2.6 | additional information | - |
additional information | for substrate cefepime, wild-type AmpC-B2 and AmpC-A, Km-value is above 1000 mM | Escherichia coli | |
3.5.2.6 | 0.1 | - |
cefotaxime | mutant AmpC-A-S287N | Escherichia coli | |
3.5.2.6 | 0.2 | - |
Imipenem | mutant AmpC-A-S287N | Escherichia coli | |
3.5.2.6 | 0.4 | - |
ceftazidime | mutant AmpC-A-S287N | Escherichia coli | |
3.5.2.6 | 0.7 | - |
cefoxitin | mutant AmpC-A-S287N | Escherichia coli | |
3.5.2.6 | 0.8 | - |
cefoxitin | mutant AmpC-B2-S287N | Escherichia coli | |
3.5.2.6 | 1 | - |
cefotaxime | mutant AmpC-B2-S287N | Escherichia coli | |
3.5.2.6 | 1 | - |
ceftazidime | mutant AmpC-B2-S287N | Escherichia coli | |
3.5.2.6 | 1 | - |
cefoxitin | wild-type AmpC-A | Escherichia coli | |
3.5.2.6 | 1.5 | - |
cefoxitin | wild-type AmpC-B2 | Escherichia coli | |
3.5.2.6 | 2 | - |
Imipenem | mutant AmpC-B2-S287N | Escherichia coli | |
3.5.2.6 | 5.5 | - |
cephalothin | mutant AmpC-A-S287N | Escherichia coli | |
3.5.2.6 | 10 | - |
Imipenem | wild-type AmpC-A | Escherichia coli | |
3.5.2.6 | 25 | - |
cefotaxime | wild-type AmpC-A | Escherichia coli | |
3.5.2.6 | 30 | - |
cephalothin | wild-type AmpC-A | Escherichia coli | |
3.5.2.6 | 32 | - |
Imipenem | wild-type AmpC-B2 | Escherichia coli | |
3.5.2.6 | 37 | - |
cefepime | mutant AmpC-B2-S287N | Escherichia coli | |
3.5.2.6 | 45 | - |
cephalothin | mutant AmpC-B2-S287N | Escherichia coli | |
3.5.2.6 | 45 | - |
cefotaxime | wild-type AmpC-B2 | Escherichia coli | |
3.5.2.6 | 60 | - |
cephaloridine | mutant AmpC-A-S287N | Escherichia coli | |
3.5.2.6 | 70 | - |
ceftazidime | wild-type AmpC-A | Escherichia coli | |
3.5.2.6 | 75 | - |
cefepime | mutant AmpC-A-S287N | Escherichia coli | |
3.5.2.6 | 85 | - |
cephalothin | wild-type AmpC-B2 | Escherichia coli | |
3.5.2.6 | 130 | - |
cephaloridine | mutant AmpC-B2-S287N | Escherichia coli | |
3.5.2.6 | 270 | - |
ceftazidime | wild-type AmpC-B2 | Escherichia coli | |
3.5.2.6 | 650 | - |
cephaloridine | wild-type AmpC-A | Escherichia coli | |
3.5.2.6 | 950 | - |
cephaloridine | wild-type AmpC-B2 | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.2.6 | Escherichia coli | P00811 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.2.6 | cefepime + H2O | - |
Escherichia coli | (2R)-2-[(R)-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-5-[(1-methylpyrrolidinium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate | - |
? | |
3.5.2.6 | cefotaxime + H2O | - |
Escherichia coli | (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
3.5.2.6 | cefoxitin + H2O | - |
Escherichia coli | (2R)-5-[(carbamoyloxy)methyl]-2-[(S)-carboxy(methoxy)[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
3.5.2.6 | ceftazidime + H2O | - |
Escherichia coli | (2R)-2-[(R)-{[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-{[(2-carboxypropan-2-yl)oxy]imino}acetyl]amino}(carboxy)methyl]-5-[(pyridin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate | - |
? | |
3.5.2.6 | cephaloridine + H2O | - |
Escherichia coli | (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate | - |
? | |
3.5.2.6 | cephalothin + H2O | - |
Escherichia coli | (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
3.5.2.6 | imipenem + H2O | - |
Escherichia coli | (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.2.6 | AmpC beta-lactamase | - |
Escherichia coli |
3.5.2.6 | AmpC-A | - |
Escherichia coli |
3.5.2.6 | AmpC-B2 | - |
Escherichia coli |
3.5.2.6 | beta-lactamase | - |
Escherichia coli |
3.5.2.6 | Cephalosporinase | - |
Escherichia coli |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.2.6 | 0.01 | - |
ceftazidime | mutant AmpC-B2-S287N | Escherichia coli | |
3.5.2.6 | 0.045 | - |
cefotaxime | mutant AmpC-B2-S287N | Escherichia coli | |
3.5.2.6 | 0.06 | - |
Imipenem | mutant AmpC-A-S287N | Escherichia coli | |
3.5.2.6 | 0.06 | - |
Imipenem | mutant AmpC-B2-S287N | Escherichia coli | |
3.5.2.6 | 0.08 | - |
cefotaxime | mutant AmpC-A-S287N | Escherichia coli | |
3.5.2.6 | 0.08 | - |
cefoxitin | mutant AmpC-B2-S287N | Escherichia coli | |
3.5.2.6 | 0.09 | - |
Imipenem | wild-type AmpC-B2 | Escherichia coli | |
3.5.2.6 | 0.1 | - |
cefoxitin | mutant AmpC-A-S287N | Escherichia coli | |
3.5.2.6 | 0.1 | - |
Imipenem | wild-type AmpC-A | Escherichia coli | |
3.5.2.6 | 0.1 | - |
cefotaxime | wild-type AmpC-B2 | Escherichia coli | |
3.5.2.6 | 0.2 | - |
ceftazidime | mutant AmpC-A-S287N | Escherichia coli | |
3.5.2.6 | 0.2 | - |
cefotaxime | wild-type AmpC-A | Escherichia coli | |
3.5.2.6 | 0.25 | - |
cefepime | mutant AmpC-B2-S287N | Escherichia coli | |
3.5.2.6 | 0.3 | - |
cefoxitin | wild-type AmpC-A | Escherichia coli | |
3.5.2.6 | 0.3 | - |
ceftazidime | wild-type AmpC-B2 | Escherichia coli | |
3.5.2.6 | 0.4 | - |
cefoxitin | wild-type AmpC-B2 | Escherichia coli | |
3.5.2.6 | 0.5 | - |
ceftazidime | wild-type AmpC-A | Escherichia coli | |
3.5.2.6 | 1.35 | - |
cefepime | mutant AmpC-A-S287N | Escherichia coli | |
3.5.2.6 | 10 | - |
cephalothin | mutant AmpC-B2-S287N | Escherichia coli | |
3.5.2.6 | 50 | - |
cephaloridine | mutant AmpC-A-S287N | Escherichia coli | |
3.5.2.6 | 65 | - |
cephalothin | mutant AmpC-A-S287N | Escherichia coli | |
3.5.2.6 | 112 | - |
cephaloridine | mutant AmpC-B2-S287N | Escherichia coli | |
3.5.2.6 | 230 | - |
cephaloridine | wild-type AmpC-A | Escherichia coli | |
3.5.2.6 | 240 | - |
cephaloridine | wild-type AmpC-B2 | Escherichia coli | |
3.5.2.6 | 500 | - |
cephalothin | wild-type AmpC-A | Escherichia coli | |
3.5.2.6 | 540 | - |
cephalothin | wild-type AmpC-B2 | Escherichia coli |