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Literature summary extracted from

  • Samuelsen, O.; Castanheira, M.; Walsh, T.R.; Spencer, J.
    Kinetic characterization of VIM-7, a divergent member of the VIM metallo-beta -lactamase family (2008), Antimicrob. Agents Chemother., 52, 2905-2908.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.2.6 expressed in Escherichia coli BL21 Pseudomonas aeruginosa

Protein Variants

EC Number Protein Variants Comment Organism
3.5.2.6 Y218F substitution may be responsible for the reduced catalytic efficiency against certain cephalosporins, including ceftazidime and cefepime Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.2.6 clavulanic acid
-
 Pseudomonas aeruginosa
3.5.2.6 additional information metallo-beta-lactamases are not inhibited by clinical beta-lactamase inhibitors Pseudomonas aeruginosa
3.5.2.6 Sulbactam
-
 Pseudomonas aeruginosa
3.5.2.6 tazobactam
-
 Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.6 0.015
-
 ampicillin
-
 Pseudomonas aeruginosa
3.5.2.6 0.017
-
 benzylpenicillin
-
 Pseudomonas aeruginosa
3.5.2.6 0.022
-
 cefotaxime
-
 Pseudomonas aeruginosa
3.5.2.6 0.026
-
 piperacillin
-
 Pseudomonas aeruginosa
3.5.2.6 0.027
-
 Imipenem
-
 Pseudomonas aeruginosa
3.5.2.6 0.028
-
 ertapenem
-
 Pseudomonas aeruginosa
3.5.2.6 0.029
-
 cefuroxime
-
 Pseudomonas aeruginosa
3.5.2.6 0.038
-
 meropenem
-
 Pseudomonas aeruginosa
3.5.2.6 0.045
-
 cephalothin
-
 Pseudomonas aeruginosa
3.5.2.6 0.058
-
 nitrocefin
-
 Pseudomonas aeruginosa
3.5.2.6 0.066
-
 azlocillin
-
 Pseudomonas aeruginosa
3.5.2.6 0.068
-
 cefoxitin
-
 Pseudomonas aeruginosa
3.5.2.6 0.075
-
 moxalactam
-
 Pseudomonas aeruginosa
3.5.2.6 0.084
-
 carbenicillin
-
 Pseudomonas aeruginosa
3.5.2.6 0.12
-
 ceftazidime
-
 Pseudomonas aeruginosa
3.5.2.6 0.25
-
 cephaloridine
-
 Pseudomonas aeruginosa
3.5.2.6 0.58
-
 cefepime
-
 Pseudomonas aeruginosa
3.5.2.6 0.86
-
 Cloxacillin
-
 Pseudomonas aeruginosa
3.5.2.6 2.7
-
 aztreonam
-
 Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.6 Pseudomonas aeruginosa Q840P9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.6 ion-exchange and gel filtration Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.6 ampicillin + H2O
-
 Pseudomonas aeruginosa (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 azlocillin + H2O
-
 Pseudomonas aeruginosa (2R,4S)-2-[(R)-carboxy({(2R)-2-[(2-oxoimidazolidine-1-carbonyl)amino]-2-phenylacetyl}amino)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 aztreonam + H2O
-
 Pseudomonas aeruginosa [(1S,2S)-1-[[(2Z)-2-(2-ammonio-1,3-thiazol-4-yl)-2-[[(2-carboxypropan-2-yl)oxy]imino]acetyl]amino]-1-carboxypropan-2-yl]sulfamate
-
 ?
3.5.2.6 benzylpenicillin + H2O
-
 Pseudomonas aeruginosa (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 carbenicillin + H2O
-
 Pseudomonas aeruginosa (2R,4S)-2-{(R)-carboxy[2-carboxy(phenyl)acetamido]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 cefepime + H2O
-
 Pseudomonas aeruginosa (2R)-2-[(R)-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-5-[(1-methylpyrrolidinium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
3.5.2.6 cefotaxime + H2O
-
 Pseudomonas aeruginosa (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cefoxitin + H2O
-
 Pseudomonas aeruginosa (2R)-5-[(carbamoyloxy)methyl]-2-[(S)-carboxy(methoxy)[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 ceftazidime + H2O
-
 Pseudomonas aeruginosa (2R)-2-[(R)-{[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-{[(2-carboxypropan-2-yl)oxy]imino}acetyl]amino}(carboxy)methyl]-5-[(pyridin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
3.5.2.6 cefuroxime + H2O
-
 Pseudomonas aeruginosa (2R)-5-[(carbamoyloxy)methyl]-2-[(R)-carboxy{[(2Z)-2-(furan-2-yl)-2-(methoxyimino)acetyl]amino}methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cephaloridine + H2O
-
 Pseudomonas aeruginosa (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
3.5.2.6 cephalothin + H2O
-
 Pseudomonas aeruginosa (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cloxacillin + H2O
-
 Pseudomonas aeruginosa (2R,4S)-2-[(R)-carboxy{[3-(2-chlorophenyl)-5-methyl-1,2-oxazole-4-carbonyl]amino}methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 ertapenem + H2O
-
 Pseudomonas aeruginosa (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({(3S,5S)-5-[(3-carboxyphenyl)carbamoyl]pyrrolidin-3-yl}sulfanyl)-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
3.5.2.6 imipenem + H2O
-
 Pseudomonas aeruginosa (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
3.5.2.6 meropenem + H2O
-
 Pseudomonas aeruginosa (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-{[(3S,5S)-5-(dimethylcarbamoyl)pyrrolidin-3-yl]sulfanyl}-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
3.5.2.6 moxalactam + H2O
-
 Pseudomonas aeruginosa (2R)-2-{(R)-carboxy[2-carboxy(4-hydroxyphenyl)acetamido]methoxymethyl}-5-{[(1-methyl-1H-tetrazol-5-yl)sulfanyl]methyl}-3,6-dihydro-2H-1,3-oxazine-4-carboxylic acid
-
 ?
3.5.2.6 nitrocefin + H2O
-
 Pseudomonas aeruginosa (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 piperacillin + H2O
-
 Pseudomonas aeruginosa (2R,4S)-2-[(R)-carboxy[[(2R)-2-[[(4-ethyl-2,3-dioxopiperazin-1-yl)carbonyl]amino]-2-phenylacetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.2.6 Cephalosporinase
-
 Pseudomonas aeruginosa
3.5.2.6 MBL
-
 Pseudomonas aeruginosa
3.5.2.6 metallo-beta-lactamase
-
 Pseudomonas aeruginosa
3.5.2.6 VIM-7
-
 Pseudomonas aeruginosa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.2.6 1.4
-
 ceftazidime
-
 Pseudomonas aeruginosa
3.5.2.6 5.3
-
 cefepime
-
 Pseudomonas aeruginosa
3.5.2.6 8
-
 ertapenem
-
 Pseudomonas aeruginosa
3.5.2.6 10
-
 cefoxitin
-
 Pseudomonas aeruginosa
3.5.2.6 16
-
 cefuroxime
-
 Pseudomonas aeruginosa
3.5.2.6 42
-
 meropenem
-
 Pseudomonas aeruginosa
3.5.2.6 56
-
 cefotaxime
-
 Pseudomonas aeruginosa
3.5.2.6 78
-
 azlocillin
-
 Pseudomonas aeruginosa
3.5.2.6 100
-
 Imipenem
-
 Pseudomonas aeruginosa
3.5.2.6 140
-
 piperacillin
-
 Pseudomonas aeruginosa
3.5.2.6 180
-
 cephalothin
-
 Pseudomonas aeruginosa
3.5.2.6 180
-
 cephaloridine
-
 Pseudomonas aeruginosa
3.5.2.6 190
-
 ampicillin
-
 Pseudomonas aeruginosa
3.5.2.6 230
-
 moxalactam
-
 Pseudomonas aeruginosa
3.5.2.6 430
-
 benzylpenicillin
-
 Pseudomonas aeruginosa
3.5.2.6 1200
-
 carbenicillin
-
 Pseudomonas aeruginosa
3.5.2.6 1500
-
 nitrocefin
-
 Pseudomonas aeruginosa
3.5.2.6 2500
-
 Cloxacillin
-
 Pseudomonas aeruginosa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5.2.6 0.74
-
 Sulbactam
-
 Pseudomonas aeruginosa
3.5.2.6 0.94
-
 clavulanic acid
-
 Pseudomonas aeruginosa
3.5.2.6 3.5
-
 tazobactam
-
 Pseudomonas aeruginosa