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Literature summary extracted from

  • Krenkova, J.; Lacher, N.A.; Svec, F.
    Highly efficient enzyme reactors containing trypsin and endoproteinase LysC immobilized on porous polymer monolith coupled to MS suitable for analysis of antibodies (2009), Anal. Chem., 81, 2004-2012.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.4.21.4 the immobilized trypsin reactor on porous polymer monoliths maintains its activity for at least 80 h with a slight decrease from 80 to 67% in sequence coverage for bovine serum albumin during this time period Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.4 acetonitrile partial inhibition at 20% acetonitrile, a 5% aqueous acetonitrile solution less likely reduces the activity of the porous polymer monolith-immobilized trypsin Bos taurus
3.4.21.4 benzamidine competitive inhibitor Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.4 Bos taurus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.4 pancreas
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.4 Bovine serum albumin + H2O
-
 Bos taurus ?
-
 ?
3.4.21.4 Cytochrome c + H2O
-
 Bos taurus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.4 25
-
 4.5 min at 25°C is sufficient for the digestion with immobilized enzyme reactor on porous polymer monoliths Bos taurus
3.4.21.4 37
-
 digestion with trypsin in solution requires a temperature of 37°C and 24 h Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.4 8 10 the optimum pH for digestion with immobilized trypsin on porous polymer monoliths is in a pH range 8-10 and comparable with that found for its soluble counterpart Bos taurus