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Literature summary extracted from

  • Gorres, K.L.; Raines, R.T.
    Direct and continuous assay for prolyl 4-hydroxylase (2009), Anal. Biochem., 386, 181-185.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.11.2 2,4-pyridine dicarboxylate
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.11.2 additional information
-
 additional information steady-state kinetics Homo sapiens
1.14.11.2 0.5
-
 PEG-Gly-Tyr-4-fluoroproline-GlyOEt pH 7.8, 22°C, recombinant enzyme, fluoride-based assay Homo sapiens
1.14.11.2 1.2
-
 PEG-Gly-Tyr-4-fluoroproline-GlyOEt pH 7.8, 22°C, recombinant enzyme, HPLC-based assay Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.2 Fe2+ P4H is a nonheme iron dioxygenase Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.11.2 procollagen L-proline + 2-oxoglutarate + O2 Homo sapiens
-
 procollagen trans-(2S,4R)-4-hydroxy-L-proline + succinate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.2 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.2 (2S,4S)-4-fluoroproline + 2-oxoglutarate + O2 development and evaluation of an assay method that continuously and directly detects turnover of the proline-containing substrate. The assay is based on (2S,4S)-4-fluoroproline, a proline analogue that is transformed into (2S)-4-oxoproline and inorganic fluoride by P4H using a fluoride ion-selective electrode for detection Homo sapiens (2S)-4-oxoproline + fluoride
-
 ?
1.14.11.2 PEG-Gly-Tyr-4-fluoroproline-GlyOEt + 2-oxoglutarate + O2
-
 Homo sapiens ?
-
 ?
1.14.11.2 procollagen L-proline + 2-oxoglutarate + O2
-
 Homo sapiens procollagen trans-(2S,4R)-4-hydroxy-L-proline + succinate + CO2
-
 ?
1.14.11.2 procollagen L-proline + 2-oxoglutarate + O2 prolyl 4-hydroxylase is a nonheme iron dioxygenase that catalyzes the posttranslational hydroxylation of (2S)-Pro residues in protocollagen strands Homo sapiens procollagen trans-(2S,4R)-4-hydroxy-L-proline + succinate + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.11.2 P4H
-
 Homo sapiens
1.14.11.2 prolyl 4-hydroxylase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.11.2 22
-
 assay at room temperature Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.11.2 4.5
-
 PEG-Gly-Tyr-4-fluoroproline-GlyOEt pH 7.8, 22°C, recombinant enzyme, fluoride-based assay Homo sapiens
1.14.11.2 6
-
 PEG-Gly-Tyr-4-fluoroproline-GlyOEt pH 7.8, 22°C, recombinant enzyme, HPLC-based assay Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.11.2 7.8
-
 assay at Homo sapiens

General Information

EC Number General Information Comment Organism
1.14.11.2 physiological function P4H is a nonheme iron dioxygenase that catalyzes the posttranslational hydroxylation of (2S)-Pro residues in protocollagen strands. The resulting (2S,4R)-4-hydroxyproline residues are essential for the folding, secretion, and stability of the collagen triple helix Homo sapiens