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Literature summary extracted from

  • Tan, Y.; Sun, L.; Xi, Z.; Yang, G.F.; Jiang, D.Q.; Yan, X.P.; Yang, X.; Li, H.Y.
    A capillary electrophoresis assay for recombinant Bacillus subtilis protoporphyrinogen oxidase (2008), Anal. Biochem., 383, 200-204.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.3.3.4 drug development protoporphyrinogen oxidase is one of the most important action targets of commercial herbicides Bacillus subtilis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.3.4 expression of His-tagged enzyme in Escherichia coli Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.3.4 acifluorfen competitive inhibition with respect to protoporphyrinogen IX Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.3.4 additional information
-
 additional information steady-state kinetics, overview Bacillus subtilis
1.3.3.4 0.007
-
 protoporphyrinogen IX pH 7.5, 25°C Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.3.4 protoporphyrinogen IX + 3 O2 Bacillus subtilis
-
 protoporphyrin + 3 H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.3.4 Bacillus subtilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.3.4 recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography Bacillus subtilis

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.3.4 protoporphyrinogen IX + 3 O2
-
 Bacillus subtilis protoporphyrin + 3 H2O2
-
 ?
1.3.3.4 protoporphyrinogen IX + 3 O2 PPO catalyzes the oxygen-dependent six-electron oxidation of protoporphyrinogen IX, i.e. protogen IX, to the fully conjugated macrocycle of protoporphyrin IX, i.e. proto IX Bacillus subtilis protoporphyrin + 3 H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.3.4 PPO
-
 Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.3.4 25
-
 assay at Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.3.4 7.5
-
 assay at Bacillus subtilis

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.3.4 FAD protoporphyrinogen oxidase is a FAD-containing enzyme Bacillus subtilis

General Information

EC Number General Information Comment Organism
1.3.3.4 malfunction when PPO is inhibited, protoporphyrin IX accumulates to cause light-dependent membrane damage Bacillus subtilis
1.3.3.4 metabolism protoporphyrinogen oxidase acts in the tetrapyrrole biosynthetic pathway that leads to the formation of both heme and chlorophylls Bacillus subtilis
1.3.3.4 physiological function protoporphyrinogen oxidase is one of the most important action targets of commercial herbicides Bacillus subtilis