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Literature summary extracted from

  • Keener, W.; Rivera, V.R.; Cho, C.Y.; Hale, M.L.; Garber, E.A.E; Poli, M.A.
    Identifitcation of the RNA N-glycosidase activity of ricin in castor bean extracts by an electrochemiluminescence-based assay (2008), Anal. Biochem., 378, 87-89.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.2.22 diagnostics differentiation of castor bean toxins from other N-glycosidase toxins depending on the signal to background ratio of the substrate GdAAA to GdAGA, castor bean extract can be distinguished from jequirity seed extract (Abrus precatorius) Ricinus communis
3.2.2.22 diagnostics differentiation of castor bean toxins from other N-glycosidase toxins depending on the signal to background ratio of the substrate GdAAA to GdAGA, castor bean extract can be distinguished from jequirity seed extract, ricin equivalent activity for jequirity seed 200-fold higher than with immunoassay Abrus precatorius

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.22 additional information Abrus precatorius removal of specific adenine of ribosomal RNA ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.22 Abrus precatorius
-
 jequirity seed
-
3.2.2.22 Ricinus communis
-
 castor bean
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.22 particle removal by centrifugation of ground material and concentration by dialysis against PEG Ricinus communis
3.2.2.22 particle removal by centrifugation of ground material and concentration by dialysis against PEG Abrus precatorius

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.2.22 seed
-
 Abrus precatorius
-
3.2.2.22 seed bean Ricinus communis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.22 5'/biotin/AGCGGGAGAGdAAAUCUCCC + H2O RNA GdAAA substrate hybridized with ruthenylated oligodeoxynucleotide 50/Ru/TTTTTdAdCdCTdCTdCTdCdGdCTdC to give a electrochemiluminescence signal Ricinus communis 5'/biotin/AGCGGGAGAG + ?
-
 ?
3.2.2.22 5'/biotin/AGCGGGAGAGdAAAUCUCCC + H2O RNA GdAAA substrate hybridized with ruthenylated oligodeoxynucleotide 50/Ru/TTTTTdAdCdCTdCTdCTdCdGdCTdC to give a electrochemiluminescence signal Abrus precatorius 5'/biotin/AGCGGGAGAG + ?
-
 ?
3.2.2.22 5'/biotin/AGCGGGAGAGdAGAUCUCCC + H2O RNA GdAGA substrate hybridized with ruthenylated oligodeoxynucleotide 50/Ru/TTTTTdAdCdCTdCTdCTdCdGdCTdC to give a electrochemiluminescence signal Ricinus communis 5'/biotin/AGCGGGAGAG + ?
-
 ?
3.2.2.22 5'/biotin/AGCGGGAGAGdAGAUCUCCC + H2O RNA GdAGA substrate hybridized with ruthenylated oligodeoxynucleotide 50/Ru/TTTTTdAdCdCTdCTdCTdCdGdCTdC to give a electrochemiluminescence signal Abrus precatorius 5'/biotin/AGCGGGAGAG + ?
-
 ?
3.2.2.22 5'/biotin/AGCGGGAGAGdUGAUCUCCC + H2O RNA GdUGA substrate hybridized with ruthenylated oligodeoxynucleotide 50/Ru/TTTTTdAdCdCTdCTdCTdCdGdCTdC to give a electrochemiluminescence signal to test for false positive signal Ricinus communis 5'/biotin/AGCGGGAGAG + ?
-
 ?
3.2.2.22 5'/biotin/AGCGGGAGAGdUGAUCUCCC + H2O RNA GdUGA substrate hybridized with ruthenylated oligodeoxynucleotide 50/Ru/TTTTTdAdCdCTdCTdCTdCdGdCTdC to give a electrochemiluminescence signal to test for false positive signal Abrus precatorius 5'/biotin/AGCGGGAGAG + ?
-
 ?
3.2.2.22 additional information removal of specific adenine of ribosomal RNA Abrus precatorius ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.2.22 abrin
-
 Abrus precatorius
3.2.2.22 ricin
-
 Ricinus communis
3.2.2.22 RNA N-glycosidase
-
 Ricinus communis
3.2.2.22 RNA N-glycosidase
-
 Abrus precatorius

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.2.22 48
-
 optimal temperature of ricin activity at 1 ng/ml based on signal-to-background ratios, 1.8fold higher than at 37°C, and 1.2fold higher than at 57°C Ricinus communis
3.2.2.22 48
-
 optimal temperature of ricin activity at 1 ng/ml based on signal-to-background ratios, 1.8fold higher than at 37°C, and 1.2fold higher than at 57°C Abrus precatorius