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Literature summary extracted from

  • Sutay Kocabas, D.; Pearson, A.R.; Phillips, S.E.; Bakir, U.; Ogel, Z.B.; McPherson, M.J.; Trinh, C.H.
    Crystallization and preliminary X-ray analysis of a bifunctional catalase-phenol oxidase from Scytalidium thermophilum (2009), Acta Crystallogr. Sect. F, 65, 486-488.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.11.1.6 hanging drop vapour diffusion method, using 24% (w/v) PEG 2000, 10 mM NaCl, 10 mM CaCl2, and 3% (w/v) 6-aminocaproic acid in 100 mM bis-Tris buffer pH 6.5 at 18°C Mycothermus thermophilus
1.14.18.1 sitting drop vapor diffusion method using 28% (w/v) PEG 1500, 100 mM bis-Tris buffer, pH 5.0, at 18°C, and hanging drop vapor diffusion method using 24% (w/v) PEG 2000, 10 mM NaCl, 10 mM CaCl2, and 3% 6-aminocaproic acid in 100 mM bis-Tris buffer, pH 6.5, at 18°C Mycothermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.18.1 additional information Mycothermus thermophilus CATPO shows both catalase and phenol oxidase activities, its major activity is the catalase-mediated decomposition of hydrogen peroxide, but it also catalyzes peroxide-independent phenol oxidation ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.6 Mycothermus thermophilus
-
 type culture Humicola insolens, ATCC 16454
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1.14.18.1 Mycothermus thermophilus
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 type culture Humicola insolens, ATCC 16454
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Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.6
-
 Mycothermus thermophilus
1.14.18.1 anion-exchange column chromatography and gel filtration Mycothermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.1 additional information CATPO shows both catalase and phenol oxidase activities, its major activity is the catalase-mediated decomposition of hydrogen peroxide, but it also catalyzes peroxide-independent phenol oxidation Mycothermus thermophilus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.6 tetramer tetramer in solution Mycothermus thermophilus
1.14.18.1 tetramer tetramer in solution Mycothermus thermophilus

Synonyms

EC Number Synonyms Comment Organism
1.11.1.6 catalase-phenol oxidase bifunctional enzyme, its major activity is the catalase-mediated decomposition of hydrogen peroxide, but it also catalyzes phenol oxidation Mycothermus thermophilus
1.11.1.6 CATPO
-
 Mycothermus thermophilus
1.14.18.1 catalase-phenol oxidase bifunctional enzyme, its major activity is the catalase-mediated decomposition of hydrogen peroxide, but it also catalyzes peroxide-independent phenol oxidation Mycothermus thermophilus
1.14.18.1 CATPO
-
 Mycothermus thermophilus