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Literature summary extracted from

  • Stsiapanava, A.; Koudelakova, T.; Lapkouski, M.; Pavlova, M.; Damborsky, J.; Smatanova, I.K.
    Crystals of DhaA mutants from Rhodococcus rhodochrous NCIMB 13064 diffracted to ultrahigh resolution: crystallization and preliminary diffraction analysis (2008), Acta Crystallogr. Sect. F, 64, 137-140.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.8.1.5 expressed in Escherichia coli BL21 cells Rhodococcus rhodochrous

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.8.1.5 mutant enzymes C176Y, I135F, and I135F/C176Y are crystallized by sitting drop vapour diffusion method, using either 80 mM bicine pH 9.0, 8% (v/v) PEG 8000, and 80 mM MgCl2 or 25% (v/v) PEG 4000, 8% 2-propanol, and 100 mM sodium acetate Rhodococcus rhodochrous

Organism

EC Number Organism UniProt Comment Textmining
3.8.1.5 Rhodococcus rhodochrous P0A3G2 NCIMB 13064
-
3.8.1.5 Rhodococcus rhodochrous NCIMB 13064 P0A3G2 NCIMB 13064
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.8.1.5 HiTrap Ni-chelating affinity resin chromatography and Superdex 75 gel filtration Rhodococcus rhodochrous

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.8.1.5 1,2,3-trichloropropane + H2O
-
 Rhodococcus rhodochrous 2,3-dichlorpropane-1-ol + chloride
-
 ir
3.8.1.5 1,2,3-trichloropropane + H2O
-
 Rhodococcus rhodochrous NCIMB 13064 2,3-dichlorpropane-1-ol + chloride
-
 ir

Synonyms

EC Number Synonyms Comment Organism
3.8.1.5 DhaA
-
 Rhodococcus rhodochrous