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Literature summary extracted from
- Structure of mannosyl-3-phosphoglycerate phosphatase from Pyrococcus horikoshii (2008), Acta Crystallogr. Sect. D, 64, 1267-1276.
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 3.1.3.70 | apo enzyme form lacking magnesium ions using the multiple-wavelength anomalous diffraction method and magnesium-bound holo enzyme form using the molecular-replacement method, with 16-20% (w/v) PEG 3350, 0.2 M ammonium chloride and 0.1 M MES-NaOH pH 6.0 | Pyrococcus horikoshii |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.3.70 | Pyrococcus horikoshii | O58690 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.3.70 | ammonium sulfate precipitation, HiTrap Ni2+-chelating column chromatography, Source 15Q column chromatography, and Superdex 75 gel filtration | Pyrococcus horikoshii |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.3.70 | 2-(alpha-D-glucosyl)-3-phosphoglycerate + H2O | - |
Pyrococcus horikoshii | 2-(alpha-D-mannosyl)-D-glycerate + phosphate | - |
ir |  |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.3.70 | MPGP | - |
Pyrococcus horikoshii |
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Release 2023.1 (January 2023)
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