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Literature summary extracted from

  • Vertessy, B.G.; Toth, J.
    Keeping uracil out of DNA: physiological role, structure and catalytic mechanism of dUTPases (2009), Acc. Chem. Res., 42, 97-106.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.6.1.23 drug development dUTPase family of enzymes are promising targets for anticancer and antimicrobial therapies Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.23 Mg2+ activates, phosphate chain coordination involves Mg2+, binding structure, overview. Mg2+ probably dissociates from the enzyme with the product PPi and not with dUMP Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.23 dUTP + H2O Homo sapiens dUTP is constantly produced in the pyrimidine biosynthesis network. To prevent uracil incorporation into DNA, representatives of the dUTP nucleotidohydrolase enzyme family eliminate excess dUTP as one moethod to avoid uracil nucleotides incorporation into DNA, besides exchanging dUTP for dTTP as a second method, overview dUMP + diphosphate dUMP is required for de novo biosynthesis of thymine ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.23 Homo sapiens P33316
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.6.1.23 dUTP + H2O = dUMP + diphosphate catalytic reaction mechanism, overview Homo sapiens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.6.1.23 carcinoma cell dUTPase is upregulated in human tumor cells Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.23 dUTP + H2O dUTP is constantly produced in the pyrimidine biosynthesis network. To prevent uracil incorporation into DNA, representatives of the dUTP nucleotidohydrolase enzyme family eliminate excess dUTP as one moethod to avoid uracil nucleotides incorporation into DNA, besides exchanging dUTP for dTTP as a second method, overview Homo sapiens dUMP + diphosphate dUMP is required for de novo biosynthesis of thymine ?
3.6.1.23 dUTP + H2O catalysis proceeds by way of an SN2 mechanism, a water molecule initiates in-line nucleophilic attack, conformational changes during the reaction take place in the C-terminus. The dUTPase binding pocket is highly specific for uracil, structural basis, overview. Phosphate chain coordination involves Mg2+ and is analogous to that of DNA polymerases. Catalytically competent and noncompetent conformation of the bound substrate, nodelling, overview Homo sapiens dUMP + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.23 homotrimer folding pattern and active site architecture, overview Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.6.1.23 dUTPase
-
 Homo sapiens
3.6.1.23 More the enzyme belongs to the dUTPase enzyme family Homo sapiens