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Literature summary extracted from

  • Gurha, P.; Gupta, R.
    Archaeal Pus10 proteins can produce both pseudouridine 54 and 55 in tRNA (2008), RNA, 14, 2521-2527.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.99.25
-
 Pyrococcus furiosus
5.4.99.25
-
 Methanocaldococcus jannaschii
5.4.99.B25
-
 Pyrococcus furiosus
5.4.99.B25
-
 Methanocaldococcus jannaschii

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.25 Methanocaldococcus jannaschii
-
-
-
5.4.99.B25 Methanocaldococcus jannaschii
-
-
-
5.4.99.25 Pyrococcus furiosus
-
-
-
5.4.99.B25 Pyrococcus furiosus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.B25 tRNA uridine54 the enzyme can function as synthase for both tRNA pseudouridine54 and pseudouridine55. The two modifications seem to occur independently. Unlike bacterial TruB and yeast Pus4, archaeal Pus10 does not require a U54*A58 reverse Hoogstein base pair and pyrimidine at position 56 to convert tRNA uridine55 to pseudouridine55. Although the T(PSI)PSI-arm of tRNA is a good substrate for both pseudouridine54 and pseudouridine55 synthesis by Mj-Pus10, the production of pseudouridine55 is more efficient than that of pseudouridine54 in this substrate. This contrasts with full-size tRNA substrates, where syntheses of both pseudouridines appear to be equally efficient Methanocaldococcus jannaschii tRNA pseudouridine54
-
 ?
5.4.99.B25 tRNA uridine54 the bifunctional enzyme can act as synthase for both tRNA pseudouridine54 and pseudouridine55. The two modifications seem to occur independently Pyrococcus furiosus tRNA pseudouridine54
-
 ?
5.4.99.25 tRNA uridine55 the bifunctional enzyme can act as synthase for both tRNA pseudouridine54 and pseudouridine55. The two modifications seem to occur independently. Unlike bacterial TruB and yeast Pus4, archaeal Pus10 does not require a U54*A58 reverse Hoogstein base pair and pyrimidine at position 56 to convert tRNA uridine55 to pseudouridine55. Although the T(PSI)PSI-arm of tRNA is a good substrate for both pseudouridine54 and pseudouridine55 synthesis by Mj-Pus10, the production of pseudouridine55 is more efficient than that of pseudouridine54 in this substrate. This contrasts with full-size tRNA substrates, where syntheses of pseudourines appear to be equally efficient Methanocaldococcus jannaschii tRNA pseudouridine55
-
 ?
5.4.99.25 tRNA uridine55 uridine55 in Haloferax volcanii tRNATrp. The bifunctional enzyme can act as synthase for both tRNA pseudouridine54 and pseudouridine55. The two modifications seem to occur independently. Unlike bacterial TruB and yeast Pus4, archaeal Pus10 does not require a U54*A58 reverse Hoogstein base pair and pyrimidine at position 56 to convert tRNA U55 to C55 Pyrococcus furiosus tRNA pseudouridine55
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.4.99.25 Mj-Pus10
-
 Methanocaldococcus jannaschii
5.4.99.B25 Mj-Pus10
-
 Methanocaldococcus jannaschii
5.4.99.25 PSI55 synthase
-
 Methanocaldococcus jannaschii
5.4.99.B25 PSI55 synthase
-
 Methanocaldococcus jannaschii
5.4.99.25 Pus10
-
 Pyrococcus furiosus
5.4.99.25 Pus10
-
 Methanocaldococcus jannaschii
5.4.99.B25 Pus10
-
 Pyrococcus furiosus
5.4.99.B25 Pus10
-
 Methanocaldococcus jannaschii