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Literature summary extracted from

  • Lee, Y.G.; Chung, K.C.; Wi, S.G.; Lee, J.C.; Bae, H.J.
    Purification and properties of a chitinase from Penicillium sp. LYG 0704 (2009), Protein Expr. Purif., 65, 244-250.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.14 Fe2+ 79% inhibition at 1 mM Penicillium sp.
3.2.1.14 Hg2+ 81% inhibition at 1 mM Penicillium sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.14 extracellular
-
 Penicillium sp.
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.14 Mg2+ 26% activation at 1 mM Penicillium sp.
3.2.1.14 Mo2+ 22% activation at 1 mM Penicillium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.14 47000
-
 x * 47000, SDS-PAGE Penicillium sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.14 chitin + H2O Penicillium sp. degradation pattern of chitin, overview ?
-
 ?
3.2.1.14 chitin + H2O Penicillium sp. LYG 0704 degradation pattern of chitin, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Penicillium sp.
-
-
-
3.2.1.14 Penicillium sp. LYG 0704
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14 native extracellular chitinase 17.6fold by isopropanol precipitation, anion exchange chromatography, and hydrophobic interaction chromatography Penicillium sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.14 12.3
-
 purified extracellular chitinase Penicillium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 chitin + H2O
-
 Penicillium sp. ?
-
 ?
3.2.1.14 chitin + H2O degradation pattern of chitin, overview Penicillium sp. ?
-
 ?
3.2.1.14 chitin + H2O
-
 Penicillium sp. LYG 0704 ?
-
 ?
3.2.1.14 chitin + H2O degradation pattern of chitin, overview Penicillium sp. LYG 0704 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 ? x * 47000, SDS-PAGE Penicillium sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 40
-
-
 Penicillium sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.14 30 60 temperature profile, overview Penicillium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 5
-
-
 Penicillium sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.14 3 8 pH profile, overview Penicillium sp.