EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.2.27 | expression of His-tagged wild-type and mutant UDGs in Escherichia coli strain BL21(DE3) | Chlamydia pneumoniae |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.2.2.27 | A205S | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Chlamydia pneumoniae |
3.2.2.27 | D77N | site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme | Chlamydia pneumoniae |
3.2.2.27 | H200Q | site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme | Chlamydia pneumoniae |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.2.27 | additional information | free uracil and abasic sites inhibit the enzyme reaction | Chlamydia pneumoniae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.2.27 | 0.0052 | - |
uracil-mismatched double-stranded DNA | pH 8.0, 37°C, recombinant wild-type enzyme | Chlamydia pneumoniae | |
3.2.2.27 | 0.009 | - |
uracil-mismatched double-stranded DNA | pH 8.0, 37°C, recombinant mutant A205S | Chlamydia pneumoniae | |
3.2.2.27 | 0.148 | - |
uracil-mismatched double-stranded DNA | pH 8.0, 37°C, recombinant mutant H200Q | Chlamydia pneumoniae | |
3.2.2.27 | 0.256 | - |
uracil-mismatched double-stranded DNA | pH 8.0, 37°C, recombinant mutant D77N | Chlamydia pneumoniae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.2.27 | Chlamydia pneumoniae | Q9Z7D3 | - |
- |
3.2.2.27 | Chlamydia pneumoniae AR39 | Q9Z7D3 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.2.27 | recombinant His-tagged wild-type and mutant UDGs from Escherichia coli strain BL21 (DE3) by nickel affinity and anion exchange chromatography | Chlamydia pneumoniae |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.2.27 | additional information | - |
purified recombinant enzyme | Chlamydia pneumoniae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.2.27 | uracil-mismatched double-stranded DNA + H2O | duplex DNA with A/U mismatch, substrate specificity, the base opposite to uracil in double strand DNAs affects uracil removal efficiencies in descending order U/-, U/T, U/C, U/G, U/A. Free uracil and abasic sites inhibit the reaction. Amino acids D77, H200, and A205 are important for the catalytic activity of UDG | Chlamydia pneumoniae | uracil + double-stranded DNA with abasic site | - |
? | |
3.2.2.27 | uracil-mismatched double-stranded DNA + H2O | duplex DNA with A/U mismatch, substrate specificity, the base opposite to uracil in double strand DNAs affects uracil removal efficiencies in descending order U/-, U/T, U/C, U/G, U/A. Free uracil and abasic sites inhibit the reaction. Amino acids D77, H200, and A205 are important for the catalytic activity of UDG | Chlamydia pneumoniae AR39 | uracil + double-stranded DNA with abasic site | - |
? | |
3.2.2.27 | uracil-mismatched double-stranded DNA with A-U mismatch + H2O | - |
Chlamydia pneumoniae | uracil + double-stranded DNA with abasic site | - |
? | |
3.2.2.27 | uracil-mismatched double-stranded DNA with A-U mismatch + H2O | - |
Chlamydia pneumoniae AR39 | uracil + double-stranded DNA with abasic site | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.2.27 | More | the enzyme is a member of the UDG family I | Chlamydia pneumoniae |
3.2.2.27 | UDG | - |
Chlamydia pneumoniae |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.2.27 | 37 | - |
- |
Chlamydia pneumoniae |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.2.27 | 0.042 | - |
uracil-mismatched double-stranded DNA | pH 8.0, 37°C, recombinant mutant D77N | Chlamydia pneumoniae | |
3.2.2.27 | 0.053 | - |
uracil-mismatched double-stranded DNA | pH 8.0, 37°C, recombinant mutant H200Q | Chlamydia pneumoniae | |
3.2.2.27 | 0.73 | - |
uracil-mismatched double-stranded DNA | pH 8.0, 37°C, recombinant mutant A205S | Chlamydia pneumoniae | |
3.2.2.27 | 5.9 | - |
uracil-mismatched double-stranded DNA with A-U mismatch + | pH 8.0, 37°C, recombinant wild-type enzyme | Chlamydia pneumoniae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.2.27 | 8 | - |
- |
Chlamydia pneumoniae |