EC Number | Cloned (Comment) | Organism |
---|---|---|
6.1.1.20 | expression of wild-type isolated B3/4 editing domain, and of the editing domain fused to the N-terminus of Escherichia coli iodoTyrRS to generate N-Eed-IYRS, overview | Escherichia coli |
6.1.1.20 | expression of wild-type isolated B3/4 editing domain, and of the editing domain fused to the N-terminus of Escherichia coli iodoTyrRS to generate N-Ped-IYRS, overview | Pyrococcus horikoshii |
6.1.1.20 | expression of wild-type isolated B3/4 editing domain, and of the editing domain fused to the N-terminus of Escherichia coli iodoTyrRS to generate N-Ted-IYRS, overview | Thermus thermophilus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
6.1.1.20 | additional information | the editing domain of PheRS is transplanted at internal sites into Escherichia coli iodoTyrRS to edit tyrosyl-tRNATyr and thereby improve the overall specificity for 3-iodo-L-tyrosine, overview | Thermus thermophilus |
6.1.1.20 | additional information | the editing domain of PheRS is transplanted at internal sites into Escherichia coli iodoTyrRS to edit tyrosyl-tRNATyr and thereby improve the overall specificity for 3-iodo-L-tyrosine, overview | Escherichia coli |
6.1.1.20 | additional information | the editing domain of PheRS is transplanted at internal sites into Escherichia coli iodoTyrRS to edit tyrosyl-tRNATyr and thereby improve the overall specificity for 3-iodo-L-tyrosine, overview | Pyrococcus horikoshii |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
6.1.1.20 | Mg2+ | - |
Thermus thermophilus | |
6.1.1.20 | Mg2+ | - |
Escherichia coli | |
6.1.1.20 | Mg2+ | - |
Pyrococcus horikoshii |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.1.1.20 | ATP + L-phenylalanine + tRNAPhe | Thermus thermophilus | - |
AMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
? | |
6.1.1.20 | ATP + L-phenylalanine + tRNAPhe | Escherichia coli | - |
AMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
? | |
6.1.1.20 | ATP + L-phenylalanine + tRNAPhe | Pyrococcus horikoshii | - |
AMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.1.1.20 | Escherichia coli | - |
- |
- |
6.1.1.20 | Pyrococcus horikoshii | - |
- |
- |
6.1.1.20 | Thermus thermophilus | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.1.1.20 | ATP + L-phenylalanine + tRNAPhe | - |
Thermus thermophilus | AMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
? | |
6.1.1.20 | ATP + L-phenylalanine + tRNAPhe | - |
Escherichia coli | AMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
? | |
6.1.1.20 | ATP + L-phenylalanine + tRNAPhe | - |
Pyrococcus horikoshii | AMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
? | |
6.1.1.20 | additional information | editing activity of the isolated recombinant B3/4 editing domain from PheRS, overview | Thermus thermophilus | ? | - |
? | |
6.1.1.20 | additional information | editing activity of the isolated recombinant B3/4 editing domain from PheRS, overview | Escherichia coli | ? | - |
? | |
6.1.1.20 | additional information | editing activity of the isolated recombinant B3/4 editing domain from PheRS, overview | Pyrococcus horikoshii | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.1.1.20 | tetramer | PheRS belongs to class IIc and is a tetrameric enzyme consisting of two alphabeta heterodimers. The B3/4 domain of the beta-subunit catalyzes the editing, domain architecture, overview | Pyrococcus horikoshii |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.1.1.20 | Phenylalanyl-tRNA synthetase | - |
Thermus thermophilus |
6.1.1.20 | Phenylalanyl-tRNA synthetase | - |
Escherichia coli |
6.1.1.20 | Phenylalanyl-tRNA synthetase | - |
Pyrococcus horikoshii |
6.1.1.20 | PheRS | - |
Thermus thermophilus |
6.1.1.20 | PheRS | - |
Escherichia coli |
6.1.1.20 | PheRS | - |
Pyrococcus horikoshii |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.1.1.20 | 37 | - |
assay at | Pyrococcus horikoshii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.1.1.20 | 7.5 | - |
assay at | Pyrococcus horikoshii |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
6.1.1.20 | ATP | - |
Thermus thermophilus | |
6.1.1.20 | ATP | - |
Escherichia coli | |
6.1.1.20 | ATP | - |
Pyrococcus horikoshii |