BRENDA - Enzyme Database

Cloning and characterization of drought-stimulated phosphatidic acid phosphatase genes from Vigna unguiculata

Franca, M.G.; Matos, A.R.; Darcy-Lameta, A.; Passaquet, C.; Lichtle, C.; Zuily-Fodil, Y.; Pham-Thi, A.T.; Plant Physiol. Biochem. 46, 1093-1100 (2008)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.3.4
additional information
the enzyme expression is drought-stimulated. Transcripts accumulate in response to water deficit, including progressive dehydration of whole plants and rapid desiccation of detached leaves. The isozymes are not affected by wounding or by treatment with jasmonic acid
Vigna unguiculata
Cloned(Commentary)
EC Number
Commentary
Organism
3.1.3.4
genes VuPAPalpha and VuPAPbeta, DNA and amino acid sequence determination and analysis, phylogenetic tree, expression of VuPAPalpha in Escherichia coli
Vigna unguiculata
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.4
additional information
the isozymes are not affected by wounding or by treatment with jasmonic acid
Vigna unguiculata
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.4
chloroplast
isozye PAPalpha contains an N-terminal transit peptide
Vigna unguiculata
9507
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.3.4
a 3-sn-phosphatidate + H2O
Vigna unguiculata
the enzyme is involved in lipid synthesis and cell signalling
a 1,2-diacyl-sn-glycerol + phosphate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.4
Vigna unguiculata
-
cv. 1183
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.4
flower
PAPalpha and PAPbeta
Vigna unguiculata
-
3.1.3.4
leaf
PAPalpha and PAPbeta
Vigna unguiculata
-
3.1.3.4
additional information
expression of isozymes PAPalpha and PAPbeta in several cowpea organs
Vigna unguiculata
-
3.1.3.4
pod
PAPalpha
Vigna unguiculata
-
3.1.3.4
root
PAPalpha and PAPbeta
Vigna unguiculata
-
3.1.3.4
seed
PAPalpha
Vigna unguiculata
-
3.1.3.4
stem
PAPalpha and PAPbeta
Vigna unguiculata
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
a 3-sn-phosphatidate + H2O
-
694740
Vigna unguiculata
a 1,2-diacyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.4
a 3-sn-phosphatidate + H2O
the enzyme is involved in lipid synthesis and cell signalling
694740
Vigna unguiculata
a 1,2-diacyl-sn-glycerol + phosphate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.3.4
More
the isozymes PAPalpha and PAPbeta contain the six transmembrane regions and the consensus sequences corresponding to the catalytic domain of the phosphatase family
Vigna unguiculata
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.3.4
30
-
assay at
Vigna unguiculata
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.4
7
-
assay at
Vigna unguiculata
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.3.4
additional information
the enzyme expression is drought-stimulated. Transcripts accumulate in response to water deficit, including progressive dehydration of whole plants and rapid desiccation of detached leaves. The isozymes are not affected by wounding or by treatment with jasmonic acid
Vigna unguiculata
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.3.4
genes VuPAPalpha and VuPAPbeta, DNA and amino acid sequence determination and analysis, phylogenetic tree, expression of VuPAPalpha in Escherichia coli
Vigna unguiculata
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.4
additional information
the isozymes are not affected by wounding or by treatment with jasmonic acid
Vigna unguiculata
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.4
chloroplast
isozye PAPalpha contains an N-terminal transit peptide
Vigna unguiculata
9507
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.3.4
a 3-sn-phosphatidate + H2O
Vigna unguiculata
the enzyme is involved in lipid synthesis and cell signalling
a 1,2-diacyl-sn-glycerol + phosphate
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.4
flower
PAPalpha and PAPbeta
Vigna unguiculata
-
3.1.3.4
leaf
PAPalpha and PAPbeta
Vigna unguiculata
-
3.1.3.4
additional information
expression of isozymes PAPalpha and PAPbeta in several cowpea organs
Vigna unguiculata
-
3.1.3.4
pod
PAPalpha
Vigna unguiculata
-
3.1.3.4
root
PAPalpha and PAPbeta
Vigna unguiculata
-
3.1.3.4
seed
PAPalpha
Vigna unguiculata
-
3.1.3.4
stem
PAPalpha and PAPbeta
Vigna unguiculata
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
a 3-sn-phosphatidate + H2O
-
694740
Vigna unguiculata
a 1,2-diacyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.4
a 3-sn-phosphatidate + H2O
the enzyme is involved in lipid synthesis and cell signalling
694740
Vigna unguiculata
a 1,2-diacyl-sn-glycerol + phosphate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.3.4
More
the isozymes PAPalpha and PAPbeta contain the six transmembrane regions and the consensus sequences corresponding to the catalytic domain of the phosphatase family
Vigna unguiculata
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.3.4
30
-
assay at
Vigna unguiculata
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.4
7
-
assay at
Vigna unguiculata