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Literature summary extracted from

  • Rasulov, B.; Copolovici, L.; Laisk, A.; Niinemets, U.
    Postillumination isoprene emission: in vivo measurements of dimethylallyldiphosphate pool size and isoprene synthase kinetics in aspen leaves (2009), Plant Physiol., 149, 1609-1618.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.27 0.0038
-
 dimethylallyl diphosphate Km of 3.8 miromol/qm are estimated from the Lineweaver-Burks plot Populus tremula x Populus tremuloides
4.2.3.27 0.26
-
 dimethylallyl diphosphate using an appropriate conversion factor between area-and volume-based units Populus tremula x Populus tremuloides

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.3.27 chloroplast
-
 Populus tremula x Populus tremuloides 9507
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.27 dimethylallyl diphosphate Populus tremula x Populus tremuloides the control on foliar isoprene emission is shared between the activity of isoprene synthase, the terminal enzyme catalyzing isoprene formation from dimethylallyl diphosphate and the pool size of dimethyl allyldiphosphate isoprene + diphosphate
-
 ?
4.2.3.27 additional information Populus tremula x Populus tremuloides dimethylallyl diphosphate pool is directly associated with isoprene synthesis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.27 Populus tremula x Populus tremuloides
-
 hybrid
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.27 leaf from one year old seedlings of hybrid aspen clone 200 which are grown under photosynthetic photon flux density of 0.5 mmol qm/s with 14 light period. Air temperature is kept at 25°C during the day and 20°C during the night, relative air humidity is 60%. Plants are watered daily with distilled water and once per weak with a combined nutrient solution containing macro- and microelements Populus tremula x Populus tremuloides
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.3.27 additional information
-
 activity of isoprene synthase remains constant during postillumination process Populus tremula x Populus tremuloides
4.2.3.27 additional information
-
 isoprene synthase mainly operates under substrate limitation and short-term light, CO2 and O2 dependencies of isoprene emission result from variation in dimethylallyl diphosphate pool size rather than from modifications in isoprene synthase activity Populus tremula x Populus tremuloides
4.2.3.27 additional information
-
 maximum capacity of isoprene synthase of Vmax of 110 nmol/qm/s are estimated Populus tremula x Populus tremuloides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.27 dimethylallyl diphosphate the control on foliar isoprene emission is shared between the activity of isoprene synthase, the terminal enzyme catalyzing isoprene formation from dimethylallyl diphosphate and the pool size of dimethyl allyldiphosphate Populus tremula x Populus tremuloides isoprene + diphosphate
-
 ?
4.2.3.27 additional information dimethylallyl diphosphate pool is directly associated with isoprene synthesis Populus tremula x Populus tremuloides ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.3.27 isoprene synthase
-
 Populus tremula x Populus tremuloides

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.3.27 7.7 8.2 broad pH-optimum Populus tremula x Populus tremuloides