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Literature summary extracted from

  • Ventriglia, T.; Kuhn, M.L.; Ruiz, M.T.; Ribeiro-Pedro, M.; Valverde, F.; Ballicora, M.A.; Preiss, J.; Romero, J.M.
    Two Arabidopsis ADP-glucose pyrophosphorylase large subunits (APL1 and APL2) are catalytic (2008), Plant Physiol., 148, 65-76.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.27 3-phosphoglycerate
-
 Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.27 apl1, DNA and amino acid determination and analysis, expression in Escherichia coli Arabidopsis thaliana
2.7.7.27 apl2, DNA and amino acid determination and analysis, expression in Escherichia coli Arabidopsis thaliana
2.7.7.27 apl3, DNA and amino acid determination and analysis, expression in Escherichia coli Arabidopsis thaliana
2.7.7.27 apl4, DNA and amino acid determination and analysis, expression in Escherichia coli Arabidopsis thaliana
2.7.7.27 aps1, DNA and amino acid determination and analysis, expression in Escherichia coli Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.27 K267R site-directed mutagenesis, co-expression of the wild-type APS1 subunit with mutated APL2K267R subunit produces enzymes with altered alpha-D-glucose-1-phosphate S0.5 Arabidopsis thaliana
2.7.7.27 K271R site-directed mutagenesis, co-expression of the wild-type APS1 subunit with mutated APL1K71R subunit produces enzymes with altered alpha-D-glucose-1-phosphate S0.5 Arabidopsis thaliana
2.7.7.27 additional information construction of a T-DNA mutant of APS1, aps1, that is starchless, lacks ADP-Glc PPase activity, APS1 mRNA, and APS1 protein, and is late flowering in long days. Transgenic lines of the aps1 mutant, expressing an inactivated form of APS1, recovered the wild-type phenotype, indicating that APL1 and APL2 have catalytic activity and may contribute to ADP-Glc synthesis in planta Arabidopsis thaliana
2.7.7.27 additional information mutation of alpha-D-glucose 1-phosphate binding site affects APL1- and APL2-dependent activity Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.27 additional information
-
 additional information wild-type and mutant kinetics, forward and reverse reaction directions Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.27 ATP + alpha-D-glucose 1-phosphate Arabidopsis thaliana
-
 ADP-D-glucose + diphosphate
-
 r
2.7.7.27 additional information Arabidopsis thaliana ADP-Glc PPase catalyzes the first committed step in starch biosynthesis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.27 Arabidopsis thaliana P55228 APS1; two genes encode small subunits APS1 and APS2, and four large subunits APL1-APL4
-
2.7.7.27 Arabidopsis thaliana P55229 APL1; two genes encode small subunits APS1 and APS2, and four large subunits APL1-APL4
-
2.7.7.27 Arabidopsis thaliana P55230 APL2; two genes encode small subunits APS1 and APS2, and four large subunits APL1-APL4
-
2.7.7.27 Arabidopsis thaliana P55231 APL3; two genes encode small subunits APS1 and APS2, and four large subunits APL1-APL4
-
2.7.7.27 Arabidopsis thaliana Q9SIK1 APL4; two genes encode small subunits APS1 and APS2, and four large subunits APL1-APL4
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.27 0.0021
-
 wild-type APS1, forward reaction Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Arabidopsis thaliana ADP-D-glucose + diphosphate
-
 r
2.7.7.27 additional information ADP-Glc PPase catalyzes the first committed step in starch biosynthesis Arabidopsis thaliana ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.27 tetramer heterotetramer alpha2beta2 consisting of two small and two large subunits, APL1 and APL2, besides their regulatory role, have catalytic activity Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
2.7.7.27 ADP-Glc PPase
-
 Arabidopsis thaliana
2.7.7.27 ADP-glucose pyrophosphorylase
-
 Arabidopsis thaliana