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Literature summary extracted from

  • Goepfert, S.; Vidoudez, C.; Tellgren-Roth, C.; Delessert, S.; Hiltunen, J.K.; Poirier, Y.
    Peroxisomal DELTA(3),DELTA(2)-enoyl CoA isomerases and evolution of cytosolic paralogues in embryophytes (2008), Plant J., 56, 728-742.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.3.8 heterologously expressed in Sacchcaromyces cerevisiae. In vivo enzyme activity tests of the proteins encoded by AtECI1, AtECI2, and AtECI3 by complementation of the Saccharomyces cerevisiae double mutant eciDELTAdci1DELTA with deletions of the genes, DELTA2,DELTA3-enoyl-CoA isomerase and DELTA3,4,DELTA2,4-dienoyl CoA isomerase Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
5.3.3.8 additional information in the absence of DELTA3,DELTA2-enoyl CoA isomerase, complete beta-oxidation of (10Z)-heptadecanoic acid is blocked. In vivo enzyme activity tests of the proteins encoded by AtECI1, AtECI2, and AtECI3 by complementation of the Saccharomyces cerevisiae double mutant eciDELTAdci1DELTA with deletions of the genes, DELTA2,DELTA3-enoyl-CoA isomerase and DELTA3,4,DELTA2,4-dienoyl CoA isomerase Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.3.3.8 cytosol determined by producing constructs between enhanced yellow fluorescent protein (EYFP) at the N-terminus and the AtECI proteins at the C-terminus. The localization of EYFP-AtECI1 and EYFP-AtECI2 indicate that AtECI1 and AtECI2 are peroxisomal proteins. The EYFP-AtECI3 construct results in diffuse fluorescence throughout the cytosol and nucleus, indicating the absence of peroxisomal targeting. AtECI1, AtECI2, and AtECI3 show high overall homology to peroximal mammalian isomerases, but have only one coserved glutamate reisdue in an position similar to that in the mitochondrial isoemrases Arabidopsis thaliana 5829
-
5.3.3.8 nucleus determined by producing constructs between enhanced yellow fluorescent protein (EYFP) at the N-terminus and the AtECI proteins at the C-terminus. The localization of EYFP-AtECI1 and EYFP-AtECI2 indicate that AtECI1 and AtECI2 are peroxisomal proteins. The EYFP-AtECI3 construct results in diffuse fluorescence throughout the cytosol and nucleus, indicating the absence of peroxisomal targeting. AtECI1, AtECI2, and AtECI3 show high overall homology to peroximal mammalian isomerases, but have only one conserved glutamate reisdue in an position similar to that in the mitochondrial isoemrases Arabidopsis thaliana 5634
-
5.3.3.8 peroxisome determined by producing constructs between enhanced yellow fluorescent protein (EYFP) at the N-terminus and the AtECI proteins at the C-terminus. The localization of EYFP-AtECI1 and EYFP-AtECI2 indicate that AtECI1 and AtECI2 are peroxisomal proteins. The EYFP-AtECI3 construct results in diffuse fluorescence throughout the cytosol and nucleus, indicating the absence of peroxisomal targeting. AtECI1, AtECI2, and AtECI3 show high overall homology to peroximal mammalian isomerases, but have only one coserved glutamate reisdue in an position similar to that in the mitochondrial isoemrases Arabidopsis thaliana 5777
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.3.8 26000
-
 AtECI1, AtECI2, AtECI3, all three proteins consist of 240 amino acids, predicted mass. AtECI2 and AtECI3 share 77% identity over the whole protein, and both are located in tandem on chromosome 4, separated by 1314 bp Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.3.8 additional information Arabidopsis thaliana enzyme participates in the degradation of unsaturated fatty acids through beta-oxidation ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.3.3.8 Arabidopsis thaliana
-
 genes AtECI1, AtECI2, AtECI3
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.3.3.8 root
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.3.8 (3E)-undecenoyl CoA intermediate of beta-oxidation of (10Z)-heptadecenoic acid. In vivo enzyme activity tests of the proteins encoded by AtECI1, AtECI2, and AtECI3 by complementation of the Saccharomyces cerevisiae double mutant eciDELTAdci1DELTA with deletions of the genes, DELTA2,DELTA3-enoyl-CoA isomerase and DELTA3,4,DELTA2,4-dienoyl CoA isomerase Arabidopsis thaliana (2E)-undecenoyl CoA
-
 ?
5.3.3.8 (3E,7Z)-tridecedienoyl CoA intermediate of beta-oxidation of 10Z-heptadecenoic acid. In vivo enzyme activity tests of the proteins encoded by AtECI1, AtECI2, and AtECI3 by complementation of the Saccharomyces cerevisiae double mutant eciDELTAdci1DELTA with deletions of the genes, DELTA2,DELTA3-enoyl-CoA isomerase and DELTA3,4,DELTA2,4-dienoyl CoA isomerase Arabidopsis thaliana (2E,7Z)-tridecedienoyl CoA
-
 ?
5.3.3.8 (3Z)-nonenoyl CoA intermediate of beta-oxidation of (10Z)-heptadecenoic acid. In vivo enzyme activity tests of the proteins encoded by AtECI1, AtECI2, and AtECI3 by complementation of the Saccharomyces cerevisiae double mutant eciDELTAdci1DELTA with deletions of the genes, DELTA2,DELTA3-enoyl-CoA isomerase and DELTA3,4,DELTA2,4-dienoyl CoA isomerase Arabidopsis thaliana (2Z)-nonenoyl CoA
-
 ?
5.3.3.8 additional information enzyme participates in the degradation of unsaturated fatty acids through beta-oxidation Arabidopsis thaliana ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.3.3.8 AtECI1
-
 Arabidopsis thaliana
5.3.3.8 AtECI2
-
 Arabidopsis thaliana
5.3.3.8 AtECI3
-
 Arabidopsis thaliana
5.3.3.8 DELTA3,DELTA2-enoyl CoA isomerase
-
 Arabidopsis thaliana
5.3.3.8 ECI
-
 Arabidopsis thaliana

pI Value

EC Number Organism Comment pI Value Maximum pI Value
5.3.3.8 Arabidopsis thaliana AtECI1
-
 6.7
5.3.3.8 Arabidopsis thaliana AtECI3
-
 7.3
5.3.3.8 Arabidopsis thaliana AtECI2
-
 8.6