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Literature summary extracted from

  • Thole, J.M.; Vermeer, J.E.; Zhang, Y.; Gadella, T.W.; Nielsen, E.
    Root hair defective4 encodes a phosphatidylinositol-4-phosphate phosphatase required for proper root hair development in Arabidopsis thaliana (2008), Plant Cell, 20, 381-395.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.B4 expressed in Escherichia coli Rosetta cells Arabidopsis thaliana
3.1.3.66 wild-type RHD4 and the mutant rhd4-2 N-terminal catalytic domains expressed from Escherichia coli Rosetta cells or BL21 cells as GST fusion proteins. EYFP-wild-type fusion protein transformed into Arabidopsis under control of the native RHD4 promoter Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.B4 G449S no activity Arabidopsis thaliana
3.1.3.66 G449S rhd4-2 mutant containing a mutation in motif VII of the SAC phosphatase domain, reduces the phosphatidylinositol-4-phosphate phosphatase activity to residual levels Arabidopsis thaliana
3.1.3.66 additional information rhd4-1 mutant, mutation results in root hairs that are shorter and randomly form bulges along their length. Stochastic loss and recovery of the RabA4b compartment in the tips of growing root hairs, altered subcellular distribution of phosphatidylinositol-4-phosphate within the root hair cell. EYFPRHD4 is capable of rescuing the rhd4-1 mutant phenotype Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.B4 membrane RHD4 is selectively recruited to RabA4blabeled membranes that are involved in polarized expansion of root hair cells Arabidopsis thaliana 16020
-
3.1.3.66 membrane RHD4 is selectively recruited to RabA4-blabeled membranes that are involved in polarized expansion of root hair cells. In conjunction with the phosphoinositide kinase PI-4Kb1, RHD4 regulates the accumulation of phosphatidylinositol-4-phosphate on membrane compartments at the tips of growing root hairs Arabidopsis thaliana 16020
-

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.B4 Arabidopsis thaliana Q9C5G5
-
-
3.1.3.66 Arabidopsis thaliana Q9C5G5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.B4 glutathione Sepharose bead chromatography Arabidopsis thaliana
3.1.3.66 by gel filtration Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.B4 root hair
-
 Arabidopsis thaliana
-
3.1.3.66 root hair
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.B4 1,2-dihexadecanoyl-sn-glycero-phospho-1-D-myo-inositol 4-phosphate + H2O
-
 Arabidopsis thaliana 1,2-dihexadecanoyl-sn-glycero-phospho-1-D-myo-inositol + phosphate
-
 ?
3.1.3.66 phosphatidylinositol-4-phosphate + H2O prefered substrate Arabidopsis thaliana ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.B4 phosphatidylinositol-4-phosphate phosphatase
-
 Arabidopsis thaliana
3.1.3.B4 PI(4)P phosphatase
-
 Arabidopsis thaliana
3.1.3.B4 RHD4
-
 Arabidopsis thaliana
3.1.3.B4 Sac1p-like phosphoinositide phosphatase
-
 Arabidopsis thaliana
3.1.3.66 phosphatidylinositol-4-phosphate phosphatase
-
 Arabidopsis thaliana
3.1.3.66 RHD4
-
 Arabidopsis thaliana
3.1.3.66 Sac1p-like phosphoinositide phosphatase
-
 Arabidopsis thaliana