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Literature summary extracted from
- C-23 hydroxylation by Arabidopsis CYP90C1 and CYP90D1 reveals a novel shortcut in brassinosteroid biosynthesis (2006), Plant Cell, 18, 3275-3288.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.14.147 | heterologous expression in the baculovirus/insect cell system | Arabidopsis thaliana |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.14.147 | 0.00106 | - |
3-epi-6-deoxocathasterone | pH 7.3, 30°C, enzyme CYP90D1 | Arabidopsis thaliana |  |
| 1.14.14.147 | 0.00494 | - |
(22S)-22-hydroxycampest-4-en-3-one | pH 7.3, 30°C | Arabidopsis thaliana | |
| 1.14.14.147 | 0.00661 | - |
3-epi-6-deoxocathasterone | pH 7.3, 30°C | Arabidopsis thaliana | |
| 1.14.14.147 | 0.00861 | - |
(22S,24R)-22-hydroxy-5alpha-ergostan-3-one | pH 7.3, 30°C | Arabidopsis thaliana | |
| 1.14.14.147 | 0.0169 | - |
6-deoxocathasterone | pH 7.3, 30°C, enzyme CYP90D1 | Arabidopsis thaliana | |
| 1.14.14.147 | 0.0181 | - |
(22S)-22-hydroxycampesterol | pH 7.3, 30°C, enzyme CYP90D1 | Arabidopsis thaliana | |
| 1.14.14.147 | 0.0194 | - |
(22S)-22-hydroxycampesterol | pH 7.3, 30°C | Arabidopsis thaliana | |
| 1.14.14.147 | 0.0359 | - |
6-deoxocathasterone | pH 7.3, 30°C | Arabidopsis thaliana | |
| 1.14.14.147 | 0.73 | - |
(22S,24R)-22-hydroxy-5alpha-ergostan-3-one | pH 7.3, 30°C, enzyme CYP90D1 | Arabidopsis thaliana | |
| 1.14.14.147 | 0.77 | - |
(22S)-22-hydroxycampest-4-en-3-one | pH 7.3, 30°C, enzyme CYP90D1 | Arabidopsis thaliana | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.147 | (22S,24R)-22-hydroxy-5alpha-ergostan-3-one + [reduced NADPH-hemoprotein reductase] + O2 | Arabidopsis thaliana | i.e (22S)-22-hydroxycampest-4-en-3-one. C-23 hydroxylation shortcuts bypass campestanol, 6-deoxocathasterone, and 6-deoxoteasterone and lead directly from (22S,24R)-22-hydroxy-5alpha-ergostan-3-one and 3-epi-6-deoxocathasterone to 3-dehydro-6-deoxoteasterone and 6-deoxotyphasterol | 3-dehydro-6-deoxoteasterone + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.147 | 3-epi-6-deoxocathasterone + [reduced NADPH-hemoprotein reductase] + O2 | Arabidopsis thaliana | C-23 hydroxylation shortcuts bypass campestanol, 6-deoxocathasterone, and 6-deoxoteasterone and lead directly from (22S,24R)-22-hydroxy-5alpha-ergostan-3-one and 3-epi-6-deoxocathasterone to 3-dehydro-6-deoxoteasterone and 6-deoxotyphasterol | 6-deoxotyphasterol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.14.147 | Arabidopsis thaliana | - |
- |
- |
| 1.14.14.147 | Arabidopsis thaliana | Q9M066 | - |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.147 | (22S)-22-hydroxycampest-4-en-3-one + [reduced NADPH-hemoprotein reductase] + O2 | - |
Arabidopsis thaliana | (22R,23R)-22,23-dihydroxycampest-4-en-3-one + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.147 | (22S)-22-hydroxycampest-4-en-3-one + [reduced NADPH-hemoprotein reductase] + O2 | i.e. (22S,24R)-22-hydroxyergost-4-en-3-one | Arabidopsis thaliana | (22R,23R)-22,23-dihydroxycampest-4-en-3-one + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.147 | (22S)-22-hydroxycampesterol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Arabidopsis thaliana | (22R,23R)-22,23-dihydroxycampesterol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.147 | (22S,24R)-22-hydroxy-5alpha-ergostan-3-one + [reduced NADPH-hemoprotein reductase] + O2 | - |
Arabidopsis thaliana | 3-dehydro-6-deoxoteasterone + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.147 | (22S,24R)-22-hydroxy-5alpha-ergostan-3-one + [reduced NADPH-hemoprotein reductase] + O2 | i.e (22S)-22-hydroxycampest-4-en-3-one. C-23 hydroxylation shortcuts bypass campestanol, 6-deoxocathasterone, and 6-deoxoteasterone and lead directly from (22S,24R)-22-hydroxy-5alpha-ergostan-3-one and 3-epi-6-deoxocathasterone to 3-dehydro-6-deoxoteasterone and 6-deoxotyphasterol | Arabidopsis thaliana | 3-dehydro-6-deoxoteasterone + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.147 | 3-epi-6-deoxocathasterone + [reduced NADPH-hemoprotein reductase] + O2 | - |
Arabidopsis thaliana | 6-deoxotyphasterol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.147 | 3-epi-6-deoxocathasterone + [reduced NADPH-hemoprotein reductase] + O2 | C-23 hydroxylation shortcuts bypass campestanol, 6-deoxocathasterone, and 6-deoxoteasterone and lead directly from (22S,24R)-22-hydroxy-5alpha-ergostan-3-one and 3-epi-6-deoxocathasterone to 3-dehydro-6-deoxoteasterone and 6-deoxotyphasterol | Arabidopsis thaliana | 6-deoxotyphasterol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.147 | 6-deoxocathasterone + [reduced NADPH-hemoprotein reductase] + O2 | - |
Arabidopsis thaliana | 6-deoxoteasterone + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.14.147 | CYP90C1/ROTUNDIFOLIA3 | - |
Arabidopsis thaliana |
| 1.14.14.147 | CYP90D1 | - |
Arabidopsis thaliana |
| 1.14.14.147 | cytochrome P450 90C1 | - |
Arabidopsis thaliana |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.147 | 30 | - |
assay at | Arabidopsis thaliana |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.14.147 | 0.0018 | - |
(22S)-22-hydroxycampesterol | pH 7.3, 30°C | Arabidopsis thaliana | |
| 1.14.14.147 | 0.002 | - |
(22S)-22-hydroxycampesterol | pH 7.3, 30°C, enzyme CYP90D1 | Arabidopsis thaliana | |
| 1.14.14.147 | 0.0023 | - |
6-deoxocathasterone | pH 7.3, 30°C | Arabidopsis thaliana | |
| 1.14.14.147 | 0.0048 | - |
6-deoxocathasterone | pH 7.3, 30°C, enzyme CYP90D1 | Arabidopsis thaliana | |
| 1.14.14.147 | 0.017 | - |
(22S)-22-hydroxycampest-4-en-3-one | pH 7.3, 30°C, enzyme CYP90D1 | Arabidopsis thaliana | |
| 1.14.14.147 | 0.018 | - |
3-epi-6-deoxocathasterone | pH 7.3, 30°C, enzyme CYP90D1 | Arabidopsis thaliana | |
| 1.14.14.147 | 0.021 | - |
(22S,24R)-22-hydroxy-5alpha-ergostan-3-one | pH 7.3, 30°C, enzyme CYP90D1 | Arabidopsis thaliana | |
| 1.14.14.147 | 0.023 | - |
(22S)-22-hydroxycampest-4-en-3-one | pH 7.3, 30°C | Arabidopsis thaliana | |
| 1.14.14.147 | 0.046 | - |
(22S,24R)-22-hydroxy-5alpha-ergostan-3-one | pH 7.3, 30°C | Arabidopsis thaliana | |
| 1.14.14.147 | 0.05 | - |
3-epi-6-deoxocathasterone | pH 7.3, 30°C | Arabidopsis thaliana | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.147 | 7.3 | - |
assay at | Arabidopsis thaliana |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.147 | cytochrome P450 | - |
Arabidopsis thaliana |
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