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Literature summary extracted from

  • Gubernator, B.; Bartoszewski, R.; Kroliczewski, J.; Wildner, G.; Szczepaniak, A.
    Ribulose-1,5-bisphosphate carboxylase/oxygenase from thermophilic cyanobacterium Thermosynechococcus elongatus (2008), Photosynth. Res., 95, 101-109.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.39 into the pUC18 plasmid for expression in Escherichia coli DH5alpha cells Thermosynechococcus vestitus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.39 0.0204
-
 D-ribulose 1,5-bisphosphate
-
 Thermosynechococcus vestitus
4.1.1.39 0.0543
-
 CO2
-
 Thermosynechococcus vestitus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.39 14000
-
 small subunit, determined by SDS-PAGE Thermosynechococcus vestitus
4.1.1.39 53000
-
 large subunit, determined by SDS-PAGE Thermosynechococcus vestitus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.39 3-phospho-D-glycerate + H+ Thermosynechococcus vestitus
-
 D-ribulose 1,5-bisphosphate + CO2 + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.39 Thermosynechococcus vestitus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.39 using a HiTrapQ DEAE sepharose and a MonoQ column Thermosynechococcus vestitus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.39 0.36
-
 purification step crude extract Thermosynechococcus vestitus
4.1.1.39 0.5
-
 purification step DEAE sepharose eluate Thermosynechococcus vestitus
4.1.1.39 1.83
-
 purification step MonoQ eluate Thermosynechococcus vestitus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.39 3-phospho-D-glycerate + H+
-
 Thermosynechococcus vestitus D-ribulose 1,5-bisphosphate + CO2 + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.39 multimer
-
 Thermosynechococcus vestitus

Synonyms

EC Number Synonyms Comment Organism
4.1.1.39 green-like type rubisco
-
 Thermosynechococcus vestitus
4.1.1.39 ribulose-1,5-bisphosphate carboxylase/oxygenase
-
 Thermosynechococcus vestitus
4.1.1.39 Rubisco
-
 Thermosynechococcus vestitus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.39 30
-
 rubisco carboxylase assay Thermosynechococcus vestitus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.39 70
-
 the enzyme is stable and active up to 70°C and deactivated at 75°C Thermosynechococcus vestitus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.39 2.65
-
 CO2
-
 Thermosynechococcus vestitus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.39 8
-
 rubisco carboxylase assay Thermosynechococcus vestitus