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Literature summary extracted from

  • Suntornthiticharoen, P.; Petmitr, S.; Chavalitshewinkoon-Petmitr, P.
    Purification and characterization of a novel 3'-5' DNA helicase from Plasmodium falciparum and its sensitivity to anthracycline antibiotics (2006), Parasitology, 133, 389-398.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.6.2.4 Aclarubicin
-
 Plasmodium falciparum
5.6.2.4 daunorubicin
-
 Plasmodium falciparum
5.6.2.4 doxorubicin
-
 Plasmodium falciparum
5.6.2.4 EDTA 5 mM Plasmodium falciparum
5.6.2.4 KCl 200 mM, inhibits Plasmodium falciparum
5.6.2.4 NaCl 200 mM, inhibits Plasmodium falciparum
5.6.2.4 Nogalamycin
-
 Plasmodium falciparum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.6.2.4 Mg2+ unwinding activity requires Mg2+ Plasmodium falciparum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.6.2.4 90000
-
 x * 90000, SDS-PAGE Plasmodium falciparum

Organism

EC Number Organism UniProt Comment Textmining
5.6.2.4 Plasmodium falciparum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.6.2.4
-
 Plasmodium falciparum

Storage Stability

EC Number Storage Stability Organism
5.6.2.4 -70°C, loses 25% of its activity following storage for 6 months Plasmodium falciparum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.6.2.4 ATP + H2O the enzyme moves unidirectionally in the 3' to 5' direction along the bound strand and prefers a fork-like substrate structure and could not unwind blunt-ended duplex DNA Plasmodium falciparum ADP + phosphate
-
 ?
5.6.2.4 dATP + H2O the enzyme moves unidirectionally in the 3' to 5' direction along the bound strand and prefers a fork-like substrate structure and could not unwind blunt-ended duplex DNA. dATP supports unwinding at 42% of the efficiency of ATP Plasmodium falciparum dADP + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
5.6.2.4 ? x * 90000, SDS-PAGE Plasmodium falciparum

Synonyms

EC Number Synonyms Comment Organism
5.6.2.4 3'-5' DNA helicase
-
 Plasmodium falciparum
5.6.2.4 3'-5' PfDH
-
 Plasmodium falciparum
5.6.2.4 PfDH A
-
 Plasmodium falciparum

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
5.6.2.4 0.002
-
 pH 9.0, 37°C Plasmodium falciparum daunorubicin
5.6.2.4 0.005
-
 pH 9.0, 37°C Plasmodium falciparum Nogalamycin
5.6.2.4 0.009
-
 pH 9.0, 37°C Plasmodium falciparum doxorubicin
5.6.2.4 0.009
-
 pH 9.0, 37°C Plasmodium falciparum Aclarubicin