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Literature summary extracted from

  • Singh, M.; Srivastava, K.K.; Bhattacharya, S.M.
    Molecular cloning and characterization of a novel immunoreactive ATPase/RNA helicase in human filarial parasite Brugia malayi (2009), Parasitol. Res., 104, 753-761.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.6.4.13 Deoxyribonucleotides ATPase activity of the bacterially expressed BmL3-helicase is triggered by both the ssRNA and the dsRNA and, to a much lesser extent, by the ssDNA and dsDNA Brugia malayi
3.6.4.13 Double-stranded DNA weak stimulation Brugia malayi
3.6.4.13 Double-stranded RNA ATPase activity of the recombinant BmL3-helicase is strongly stimulated by dsRNA Brugia malayi
3.6.4.13 ribonucleotides ATPase activity of the bacterially expressed BmL3-helicase is triggered by both the ssRNA and the dsRNA and, to a much lesser extent, by the ssDNA and dsDNA, recombinant BmL3-helicase is strongly stimulated by dsRNA Brugia malayi
3.6.4.13 single-stranded DNA weak stimulation Brugia malayi
3.6.4.13 Single-stranded RNA stimulates Brugia malayi

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.4.13 expression of the N-terminally His-tagged enzyme as soluble protein in Escherichia coli strain BL21(DE3) Brugia malayi
3.6.4.13 overexpressed as His-tag fusion protein in Escherichia coli Brugia malayi

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.4.13 Mg2+ activates Brugia malayi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.4.13 ATP + H2O Brugia malayi
-
 ADP + phosphate
-
 ?
3.6.4.13 additional information Brugia malayi DEAD box proteins are putative RNA unwinding proteins, BmL3-helicase also is a DEAD box RNA helicase ?
-
 ?
3.6.4.13 RNA + H2O Brugia malayi helicase/unwinding activity, either ATP or dATP is required for the unwinding activity ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.4.13 Brugia malayi
-
-
-
3.6.4.13 Brugia malayi EF409381
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.4.13 recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Brugia malayi

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.6.4.13 larva
-
 Brugia malayi
-
3.6.4.13 additional information presence of BmL3-helicase in various life stages of Brugia malayi Brugia malayi
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.4.13 ATP + H2O
-
 Brugia malayi ADP + phosphate
-
 ?
3.6.4.13 ATP + H2O either ATP or dATP is required for the unwinding activity Brugia malayi ADP + phosphate
-
 ?
3.6.4.13 dATP + H2O either ATP or dATP is required for the unwinding activity Brugia malayi dADP + phosphate
-
 ?
3.6.4.13 additional information DEAD box proteins are putative RNA unwinding proteins, BmL3-helicase also is a DEAD box RNA helicase Brugia malayi ?
-
 ?
3.6.4.13 RNA + H2O helicase/unwinding activity, either ATP or dATP is required for the unwinding activity Brugia malayi ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.6.4.13 ATP/dATP-dependent RNA helicase
-
 Brugia malayi
3.6.4.13 ATPase/RNA helicase
-
 Brugia malayi
3.6.4.13 BmL3-helicase
-
 Brugia malayi
3.6.4.13 DEAD box RNA helicase
-
 Brugia malayi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.4.13 37
-
 assay at Brugia malayi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.4.13 7.5
-
 assay at Brugia malayi