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Literature summary extracted from

  • Humbert, O.; Salama, N.R.
    The Helicobacter pylori HpyAXII restriction-modification system limits exogenous DNA uptake by targeting GTAC sites but shows asymmetric conservation of the DNA methyltransferase and restriction endonuclease components (2008), Nucleic Acids Res., 36, 6893-6906.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.21.3 additional information an active R.HpyAXII endonuclease can only exist in the presence of its active DNA MTase partner Helicobacter pylori

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.72 expressed in Escherichia coli ER2925(DE3) cells Helicobacter pylori
3.1.21.3 pCR-TOPO/hpyAXII digested with EcoRI and subcloned into plasmid Bluescript SK+. Plasmid pET15b/hpyAXIIR expressed in Escherichia coli strain BL21CodonPlus(DE3)-RIL Helicobacter pylori
3.1.21.4 expressed in Escherichia coli strains ER2925(DE3) and BL21(DE3) Helicobacter pylori

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.72 S-adenosyl-L-methionine + DNA adenine Helicobacter pylori M.HpyAXII targets GTAC sites S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
 ?
2.1.1.72 S-adenosyl-L-methionine + DNA adenine Helicobacter pylori NSH57 M.HpyAXII targets GTAC sites S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
 ?
3.1.21.3 DNA + H2O Helicobacter pylori R.HpyAXII effectively restricts chromosomal DNA during natural transformation ?
-
 ?
3.1.21.3 DNA + H2O Helicobacter pylori NSH57 R.HpyAXII effectively restricts chromosomal DNA during natural transformation ?
-
 ?
3.1.21.3 plasmid DNA + H2O Helicobacter pylori R.HpyAXII effectively restricts unmethylated plasmid during natural transformation ?
-
 ?
3.1.21.3 plasmid DNA + H2O Helicobacter pylori NSH57 R.HpyAXII effectively restricts unmethylated plasmid during natural transformation ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.72 Helicobacter pylori
-
-
-
2.1.1.72 Helicobacter pylori NSH57
-
-
-
3.1.21.3 Helicobacter pylori
-
 strains 26695, J99, HPAG1, NSH57and NSH79
-
3.1.21.4 Helicobacter pylori B6ED46 fragment
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.72 S-adenosyl-L-methionine + DNA adenine M.HpyAXII targets GTAC sites Helicobacter pylori S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
 ?
2.1.1.72 S-adenosyl-L-methionine + DNA adenine M.HpyAXII targets GTAC sites Helicobacter pylori NSH57 S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
 ?
3.1.21.3 DNA + H2O R.HpyAXII effectively restricts chromosomal DNA during natural transformation Helicobacter pylori ?
-
 ?
3.1.21.3 DNA + H2O R.HpyAXII effectively restricts chromosomal DNA during natural transformation Helicobacter pylori NSH57 ?
-
 ?
3.1.21.3 plasmid DNA + H2O R.HpyAXII effectively restricts unmethylated plasmid during natural transformation Helicobacter pylori ?
-
 ?
3.1.21.3 plasmid DNA + H2O R.HpyAXII effectively restricts unmethylated plasmid during natural transformation Helicobacter pylori NSH57 ?
-
 ?
3.1.21.4 DNA + H2O HpyAXII effectively restricts both unmethylated plasmid and chromosomal DNA during natural transformation, the enzyme targets the tetramer 5'-GTAC-3' Helicobacter pylori double-stranded DNA fragments with terminal 5'-phosphates
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.72 gamma-adenine MTase
-
 Helicobacter pylori
2.1.1.72 HpyAXII consists of a restriction endonuclease and a DNA methyltransferase Helicobacter pylori
2.1.1.72 M.HpyAXII
-
 Helicobacter pylori
3.1.21.3 HpyAXII restriction-modification system
-
 Helicobacter pylori
3.1.21.3 R.HpyAXII
-
 Helicobacter pylori
3.1.21.3 type II R-M system
-
 Helicobacter pylori
3.1.21.4 HpyAXII
-
 Helicobacter pylori
3.1.21.4 type II restriction endonuclease
-
 Helicobacter pylori

General Information

EC Number General Information Comment Organism
2.1.1.72 malfunction deletion of the methyltransferase M.HpyAXII is lethal when associated with an active endonuclease, M.HpyAXII MTase activity is not required for establishing infection in mice Helicobacter pylori