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Literature summary extracted from

  • Gu, Y.; Masuda, Y.; Kamiya, K.
    Biochemical analysis of human PIF1 helicase and functions of its N-terminal domain (2008), Nucleic Acids Res., 36, 6295-6308.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.6.2.3 single-stranded DNA nonstructural single-stranded DNA greatly stimulates ATPase activity due to a high affinity for PIF1, even though PIF1 preferentially unwinds forked substrates. The N-terminal portion oF PIF1 helicase, named the PIF1 N-terminal (PINT) domain, contributes to enhancing the interaction with single-stranded DNA through intrinsic binding activity Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.6.2.3 replication protein A inhibits unwinding and annealing activities Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.6.2.3 0.0038
-
 ATP pH 8.0, 30°C, with saturating concentration of M13 mp18 ssDNA Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.6.2.3 71000
-
 1 * 71000, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
5.6.2.3 Homo sapiens Q9H611
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.6.2.3 full-length PIF1 with a 6* histidine tag at the N-terminus, a C-terminal truncated form (PIF1N) and a N-terminal truncated form (PIF1C) Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.6.2.3 ATP + H2O
-
 Homo sapiens ADP + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
5.6.2.3 monomer 1 * 71000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
5.6.2.3 PIF1
-
 Homo sapiens
5.6.2.3 PIF1 helicase
-
 Homo sapiens