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Literature summary extracted from
- The RNase E of Escherichia coli is a membrane-binding protein (2008), Mol. Microbiol., 70, 799-813.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.26.12 | expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.1.26.12 | N305D | the mutation is localized in the catalytic domain of RNase E | Escherichia coli |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.26.12 | membrane | localized to the inner cytoplasmic membrane, segment-A is necessary and sufficient for RNase E binding to membranes | Escherichia coli | 16020 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.26.12 | 464000 | - |
co-sedimentation | Escherichia coli |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.26.12 | Escherichia coli | - |
- |
- |
| 3.1.26.12 | Escherichia coli KSL2000 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.26.12 | Ni-NTA column chromatography | Escherichia coli |
Renatured (Commentary)
| EC Number | Renatured (Comment) | Organism |
|---|---|---|
| 3.1.26.12 | RNase E is renatured on the Ni-NTA resin by decreasing the concentration of urea to 0.8 M | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.26.12 | 9SA RNA + H2O | 9Sa is a fragment of the 9S precursor of 5S rRNA | Escherichia coli | ? | - |
? |  |
| 3.1.26.12 | 9SA RNA + H2O | 9Sa is a fragment of the 9S precursor of 5S rRNA | Escherichia coli KSL2000 | ? | - |
? | |
| 3.1.26.12 | RNA + H2O | - |
Escherichia coli | ? | - |
? | |
| 3.1.26.12 | RNA + H2O | - |
Escherichia coli KSL2000 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.26.12 | tetramer | co-sedimentation | Escherichia coli |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.26.12 | RNase E | RNase E is an essential endoribonuclease involved in RNA processing and mRNA degradation | Escherichia coli |
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Release 2023.1 (January 2023)
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