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Literature summary extracted from

  • Purcarea, C.; Fernando, R.; Evans, H.G.; Evans, D.R.
    The sole serine/threonine protein kinase and its cognate phosphatase from Aquifex aeolicus targets pyrimidine biosynthesis (2008), Mol. Cell. Biochem., 311, 199-213.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.1 expressed in Escherichia coli as a His-tagged fusion protein Aquifex aeolicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.1 0.46
-
 GTP autophosphorylation Aquifex aeolicus
2.7.11.1 0.64
-
 ATP autophosphorylation Aquifex aeolicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.1 34000
-
 SDS-PAGE Aquifex aeolicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.1 Aquifex aeolicus O66844
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.11.1 phosphoprotein enzyme is autophosphorylated on a threonine residue Aquifex aeolicus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.1 using Ni2+-affinity chromatography Aquifex aeolicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.1 ATP + AaSTPK enzyme is autophosphorylated on a threonine residue Aquifex aeolicus ADP + phosphorylated-AaSTPK
-
 ?
2.7.11.1 ATP + CPS.A subunit AaSTPK also phosphorylates one threonine residue on the carbamoyl phosphate synthetase, CPS.A subunit Aquifex aeolicus ADP + phosphorylated-CPS.A subunit
-
 ?
2.7.11.1 GTP + AaSTPK enzyme is autophosphorylated on a threonine residue Aquifex aeolicus GDP + phosphorylated-AaSTPK
-
 ?
2.7.11.1 GTP + CPS.A subunit
-
 Aquifex aeolicus GDP + phosphorylated-CPS.A subunit
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.1 monomer gel filtration Aquifex aeolicus

Synonyms

EC Number Synonyms Comment Organism
2.7.11.1 AaSTPK
-
 Aquifex aeolicus
2.7.11.1 serine-threonine kinase
-
 Aquifex aeolicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.1 50
-
 assay at Aquifex aeolicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.11.1 0.00006
-
 GTP autophosphorylation Aquifex aeolicus
2.7.11.1 0.00016
-
 ATP autophosphorylation Aquifex aeolicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.1 6.8
-
 assay at Aquifex aeolicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.1 MgATP2- preferred substrate Aquifex aeolicus
2.7.11.1 MnATP2-
-
 Aquifex aeolicus
2.7.11.1 MnGTP2-
-
 Aquifex aeolicus