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Literature summary extracted from

  • Sculaccio, S.A.; Rodrigues, E.M.; Cordeiro, A.T.; Magalhaes, A.; Braga, A.L.; Alberto, E.E.; Thiemann, O.H.
    Selenocysteine incorporation in kinetoplastid: selenophosphate synthetase (SELD) from Leishmania major and Trypanosoma brucei (2008), Mol. Biochem. Parasitol., 162, 165-171.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.9.3 gene selD, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strain BL21(DE3), and functional complementation of Escherichia coli SelD deletion strain WL400 Trypanosoma brucei
2.7.9.3 gene selD, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strain BL21(DE3), and functional complementation of Escherichia coli SelD deletion strain WL400 Leishmania major

Protein Variants

EC Number Protein Variants Comment Organism
2.7.9.3 C42A site-directed mutagenesis Trypanosoma brucei
2.7.9.3 C46A site-directed mutagenesis Leishmania major

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.9.3 kinetoplast
-
 Trypanosoma brucei 20023
-
2.7.9.3 kinetoplast
-
 Leishmania major 20023
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.9.3 Mg2+
-
 Trypanosoma brucei
2.7.9.3 Mg2+
-
 Leishmania major

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.9.3 42700
-
 2 * 42700, about, sequence calculation, 2 * 45000, recombinant SelD, SDS-PAGE Leishmania major
2.7.9.3 43000
-
 2 * 43000, about, sequence calculation, 2 * 45000, recombinant SelD, SDS-PAGE Trypanosoma brucei
2.7.9.3 45000
-
 2 * 42700, about, sequence calculation, 2 * 45000, recombinant SelD, SDS-PAGE Leishmania major
2.7.9.3 45000
-
 2 * 43000, about, sequence calculation, 2 * 45000, recombinant SelD, SDS-PAGE Trypanosoma brucei
2.7.9.3 100000
-
 about, gel filtration Trypanosoma brucei
2.7.9.3 100000
-
 about, gel filtration Leishmania major

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.9.3 ATP + selenide Trypanosoma brucei selenophosphate synthetase produces the biologically active selenium donor compound, monoselenophosphate, from ATP and selenide, for the synthesis of selenocysteine AMP + selenophosphate + phosphate
-
 ?
2.7.9.3 ATP + selenide Leishmania major selenophosphate synthetase produces the biologically active selenium donor compound, monoselenophosphate, from ATP and selenide, for the synthesis of selenocysteine AMP + selenophosphate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.9.3 Leishmania major Q4Q0M0 gene selD
-
2.7.9.3 Trypanosoma brucei
-
 gene selD
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.9.3 recombinant SelD from Escherichia coli strain BL21(DE3) to homogeneity Trypanosoma brucei
2.7.9.3 recombinant SelD from Escherichia coli strain BL21(DE3) to homogeneity Leishmania major

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.9.3 epimastigote cell culture Trypanosoma brucei
-
2.7.9.3 epimastigote cell culture Leishmania major
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.9.3 ATP + selenide
-
 Trypanosoma brucei AMP + selenophosphate + phosphate
-
 ?
2.7.9.3 ATP + selenide
-
 Leishmania major AMP + selenophosphate + phosphate
-
 ?
2.7.9.3 ATP + selenide selenophosphate synthetase produces the biologically active selenium donor compound, monoselenophosphate, from ATP and selenide, for the synthesis of selenocysteine Trypanosoma brucei AMP + selenophosphate + phosphate
-
 ?
2.7.9.3 ATP + selenide selenophosphate synthetase produces the biologically active selenium donor compound, monoselenophosphate, from ATP and selenide, for the synthesis of selenocysteine Leishmania major AMP + selenophosphate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.9.3 dimer 2 * 42700, about, sequence calculation, 2 * 45000, recombinant SelD, SDS-PAGE Leishmania major
2.7.9.3 dimer 2 * 43000, about, sequence calculation, 2 * 45000, recombinant SelD, SDS-PAGE Trypanosoma brucei

Synonyms

EC Number Synonyms Comment Organism
2.7.9.3 SelD
-
 Trypanosoma brucei
2.7.9.3 SelD
-
 Leishmania major
2.7.9.3 selenophosphate synthetase
-
 Trypanosoma brucei
2.7.9.3 selenophosphate synthetase
-
 Leishmania major

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.9.3 30
-
 assay at Trypanosoma brucei
2.7.9.3 30
-
 assay at Leishmania major

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.9.3 7.2
-
 assay at Trypanosoma brucei
2.7.9.3 7.2
-
 assay at Leishmania major

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.9.3 ATP
-
 Trypanosoma brucei
2.7.9.3 ATP
-
 Leishmania major

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.9.3 Leishmania major isoelectric focusing, recombinant enzyme
-
 6.05
2.7.9.3 Trypanosoma brucei isoelectric focusing, recombinant enzyme
-
 6.34