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Literature summary extracted from

  • Mueller, R.H.; Babel, W.
    3-Hexulose-6-phosphate synthase from Acetobacter methanolicus MB58 (1990), Methods Enzymol., 188, 401-405.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.2.43 Cu2+ 1 mM Cu2+ is inhibitory, and the effect is partly abolished by Mg2+ Acidomonas methanolica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.2.43 Ca2+ 1 mM Ca2+ is less than 50% less effective in activity stimulation than 1 mM Mg2+ Acidomonas methanolica
4.1.2.43 Co2+ 1 mM Co2+ is less than 50% less effective in activity stimulation than 1 mM Mg2+ Acidomonas methanolica
4.1.2.43 Fe2+ 1 mM Fe2+ is less than 50% less effective in activity stimulation than 1 mM Mg2+ Acidomonas methanolica
4.1.2.43 Mg2+ bivalent cations are essential for activity, most effective is Mg2+ Acidomonas methanolica
4.1.2.43 Mn2+ 1 mM Mn2+ is 20% less effective in activity stimulation than 1 mM Mg2+ Acidomonas methanolica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.43 20400
-
 4 * 20400, SDS-PAGE Acidomonas methanolica
4.1.2.43 80000
-
 gel filtration Acidomonas methanolica

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.43 Acidomonas methanolica
-
-
-
4.1.2.43 Acidomonas methanolica MB58
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.43 ammonium sulfate fractionation, DEAE-cellulose column chromatography, and Sephadex G-100 gel filtration Acidomonas methanolica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.2.43 1.4
-
 cell free extract, at 30°C Acidomonas methanolica
4.1.2.43 64
-
 after 46fold purification, at 30°C Acidomonas methanolica

Storage Stability

EC Number Storage Stability Organism
4.1.2.43 -20°C, purified enzyme, at least 1 year, no loss of activity Acidomonas methanolica
4.1.2.43 0°C to -4°C, purified enzyme in the presence of 3.2 M ammonium sulfate, at least 1 year, no loss of activity Acidomonas methanolica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.43 D-ribulose 5-phosphate + formaldehyde
-
 Acidomonas methanolica D-arabino-hex-3-ulose 6-phosphate
-
 r
4.1.2.43 D-ribulose 5-phosphate + formaldehyde
-
 Acidomonas methanolica MB58 D-arabino-hex-3-ulose 6-phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
4.1.2.43 homotetramer 4 * 20400, SDS-PAGE Acidomonas methanolica

Synonyms

EC Number Synonyms Comment Organism
4.1.2.43 HPS
-
 Acidomonas methanolica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.2.43 60
-
-
 Acidomonas methanolica

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.2.43 40 60 no decrease in activity is observed within 6 h at 40°C in the presence of 5 mM MgCl2, at 50°C and 60°C the enzyme is stable for 2.5 and 0.5 h, respectively, one-half the initial activities are found after 6 and 1.5 h respectively Acidomonas methanolica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.43 7 8
-
 Acidomonas methanolica