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Literature summary extracted from
- Phosphomannose isomerase and phosphomannomutase gene disruptions in Streptomyces nodosus: impact on amphotericin biosynthesis and implications for glycosylation engineering (2009), Metab. Eng., 11, 40-47.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 5.3.1.8 | synthesis | inactivation of the manA gene encoding phosphomannose isomerase results in production of amphotericins and their aglycones, 8-deoxyamphoteronolides. A double mutant lacking the phosphomannose isomerase and phosphomannomutase genes produces 8-deoxyamphoteronolides in good yields along with trace levels of glycosylated amphotericins | Streptomyces nodosus |
| 5.4.2.8 | biotechnology | the double mutant lacking the PMI and PMM genes produces 8-deoxyamphoteronolides in good yields along with trace levels of glycosylated amphotericins, with further genetic engineering these mutants may activate alternative hexoses as GDP-sugars for transfer to aglycones in vivo | Streptomyces nodosus |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.3.1.8 | - |
Streptomyces nodosus |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 5.3.1.8 | additional information | inactivation of the manA gene encoding phosphomannose isomerase results in production of amphotericins and their aglycones, 8-deoxyamphoteronolides. A double mutant lacking the phosphomannose isomerase and phosphomannomutase genes produces 8-deoxyamphoteronolides in good yields along with trace levels of glycosylated amphotericins | Streptomyces nodosus |
| 5.4.2.8 | DELTAmanAB | phosphomannose isomerase, phosphomannosemutase double mutant | Streptomyces nodosus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.2.8 | alpha-D-mannose 1-phosphate | Streptomyces nodosus | - |
D-mannose 6-phosphate | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.3.1.8 | Streptomyces nodosus | - |
- |
- |
| 5.4.2.8 | Streptomyces nodosus | B6Z254 | - |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.2.8 | alpha-D-mannose 1-phosphate | - |
Streptomyces nodosus | D-mannose 6-phosphate | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.4.2.8 | phosphomannomutase | - |
Streptomyces nodosus |
| 5.4.2.8 | PMM | - |
Streptomyces nodosus |
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Release 2023.1 (January 2023)
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