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Literature summary extracted from

  • Gruez, A.; Selisko, B.; Roberts, M.; Bricogne, G.; Bussetta, C.; Jabafi, I.; Coutard, B.; De Palma, A.M.; Neyts, J.; Canard, B.
    The crystal structure of coxsackievirus B3 RNA-dependent RNA polymerase in complex with its protein primer VPg confirms the existence of a second VPg binding site on Picornaviridae polymerases (2008), J. Virol., 82, 9577-9590.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.7.48 in complex with its protein primer VPg and diphosphate, hanging drop vapour diffusion method, in 20 mM Tris (pH 9.0), 300 mM NaCl, 15% glycerol, 0.5 mM Tris(2-carboxyethyl)phosphine Coxsackievirus B3

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.48 Mg2+ optimum concentration at 0.8 mM Coxsackievirus B3
2.7.7.48 Mn2+ optimum concentration at 0.1 mM, RdRp is twice as active at the Mn2+ concentration optimum compared to that of Mg2+ Coxsackievirus B3

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.48 Coxsackievirus B3
-
-
-
2.7.7.48 Coxsackievirus B3 Nancy
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.48 nucleoside triphosphate + RNAn using poly(rA)/(dT)15 as a template-primer system Coxsackievirus B3 diphosphate + RNAn+1
-
 ?
2.7.7.48 nucleoside triphosphate + RNAn using poly(rA)/(dT)15 as a template-primer system Coxsackievirus B3 Nancy diphosphate + RNAn+1
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.48 3Dpol
-
 Coxsackievirus B3
2.7.7.48 RDRP
-
 Coxsackievirus B3
2.7.7.48 RNA-dependent RNA polymerase
-
 Coxsackievirus B3