Literature summary extracted from

Ohlsson, L.; Hjelte, L.; Huehn, M.; Scholte, B.J.; Wilke, M.; Flodstroem-Tullberg, M.; Nilsson, A.
Expression of intestinal and lung alkaline sphingomyelinase and neutral ceramidase in cystic fibrosis f508del transgenic mice (2008), J. Pediatr. Gastroenterol. Nutr., 47, 547-554.

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.1.4.12	Mus musculus	-	-	-
3.5.1.23	Mus musculus	-	F508del mouse, mouse model with well-characterized intestinal pathology	-

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
3.1.4.12	colonic mucosa	alk-SMase	Mus musculus	-
3.1.4.12	kidney	-	Mus musculus	-
3.1.4.12	liver	-	Mus musculus	-
3.1.4.12	lung	acid-SMase activity is comparable in lung and intestine, alk-ASMase activity is lower in lung than in intestine	Mus musculus	-
3.1.4.12	additional information	no detectable activity of acid-SMase in colonic mucosa	Mus musculus	-
3.1.4.12	small intestine mucosa	acid-SMase activity is comparable in lung and intestine	Mus musculus	-
3.1.4.12	spleen	-	Mus musculus	-
3.5.1.23	gut	-	Mus musculus	-
3.5.1.23	intestine	-	Mus musculus	-
3.5.1.23	kidney	-	Mus musculus	-
3.5.1.23	liver	-	Mus musculus	-
3.5.1.23	lung	-	Mus musculus	-
3.5.1.23	mucosa	-	Mus musculus	-
3.5.1.23	spleen	-	Mus musculus	-

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
3.5.1.23	0.000000053	-	homozygous F508del mice, lung	Mus musculus
3.5.1.23	0.000000055	-	wild-type mice, lung	Mus musculus
3.5.1.23	0.000000056	-	heterozygous F508del mice, lung	Mus musculus
3.5.1.23	0.000002	-	heterozygous F508del mice, spleen	Mus musculus
3.5.1.23	0.000002	-	wild-type mice, spleen	Mus musculus
3.5.1.23	0.0000028	-	homozygous F508del mice, spleen	Mus musculus
3.5.1.23	0.0000035	-	wild-type mice, lung	Mus musculus
3.5.1.23	0.00000375	-	heterozygous F508del mice, lung	Mus musculus
3.5.1.23	0.00000416	-	homozygous F508del mice, lung	Mus musculus
3.5.1.23	0.00001	-	homozygous F508del mice, mucosal homogenates	Mus musculus
3.5.1.23	0.0000127	-	heterozygous F508del mice, mucosal homogenates	Mus musculus
3.5.1.23	0.0000137	-	heterozygous F508del mice, mucosal homogenates	Mus musculus
3.5.1.23	0.0000145	-	homozygous F508del mice, mucosal homogenates	Mus musculus
3.5.1.23	0.0000155	-	wild-type mice, mucosal homogenates	Mus musculus
3.5.1.23	0.0000163	-	wild-type mice, mucosal homogenates	Mus musculus
3.5.1.23	0.0000167	-	wild-type mice, mucosal homogenates	Mus musculus
3.5.1.23	0.0000187	-	wild-type mice, kidney	Mus musculus
3.5.1.23	0.0000188	-	heterozygous F508del mice, mucosal homogenates	Mus musculus
3.5.1.23	0.0000192	-	homozygous F508del mice, mucosal homogenates	Mus musculus
3.5.1.23	0.0000193	-	homozygous F508del mice, kidney	Mus musculus
3.5.1.23	0.00002	-	homozygous F508del mice, mucosal homogenates	Mus musculus
3.5.1.23	0.0000208	-	heterozygous F508del mice, kidney	Mus musculus
3.5.1.23	0.0000208	-	homozygous F508del mice, mucosal homogenates	Mus musculus
3.5.1.23	0.0000215	-	wild-type mice, liver	Mus musculus
3.5.1.23	0.0000227	-	heterozygous F508del mice, mucosal homogenates	Mus musculus
3.5.1.23	0.000023	-	wild-type mice, mucosal homogenates	Mus musculus
3.5.1.23	0.0000238	-	homozygous F508del mice, liver	Mus musculus
3.5.1.23	0.0000255	-	heterozygous F508del mice, liver	Mus musculus
3.5.1.23	0.0000272	-	heterozygous F508del mice, mucosal homogenates	Mus musculus
3.5.1.23	0.0000273	-	wild-type mice, mucosal homogenates	Mus musculus
3.5.1.23	0.0000292	-	homozygous F508del mice, mucosal homogenates	Mus musculus
3.5.1.23	0.0000308	-	heterozygous F508del mice, mucosal homogenates	Mus musculus
3.5.1.23	0.0000417	-	wild-type mice, mucosal homogenates	Mus musculus
3.5.1.23	0.000048	-	wild-type mice, mucosal homogenates	Mus musculus
3.5.1.23	0.0000542	-	homozygous F508del mice, mucosal homogenates	Mus musculus
3.5.1.23	0.0000625	-	heterozygous F508del mice, mucosal homogenates	Mus musculus
3.5.1.23	0.0001	-	heterozygous F508del mice, mucosal homogenates	Mus musculus

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.1.4.12	sphingomyelin + H2O	-	Mus musculus	N-acylsphingosine + choline phosphate	-	?
3.5.1.23	octanoyl-D-erythro-sphingosine + H2O	substrate activity assay	Mus musculus	?	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
3.1.4.12	acid sphingomyelinase	-	Mus musculus
3.1.4.12	acid-SMase	-	Mus musculus
3.1.4.12	alk-SMase	-	Mus musculus
3.1.4.12	alkaline sphingomyelinase	-	Mus musculus
3.5.1.23	acid ceramidase	-	Mus musculus
3.5.1.23	CDase	-	Mus musculus
3.5.1.23	neutral ceramidase	-	Mus musculus

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
3.5.1.23	37	-	activity assay	Mus musculus

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
3.5.1.23	5	-	activity assay	Mus musculus
3.5.1.23	7	-	activity assay	Mus musculus