Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Chen, C.N.; Chin, K.H.; Wang, A.H.; Chou, S.H.
    The first crystal structure of gluconolactonase important in the glucose secondary metabolic pathways (2008), J. Mol. Biol., 384, 604-614.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.17 expressed in Escherichia coli BL21 (DE3) pLysS cells Xanthomonas campestris

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.1.17 sitting drop vapour diffusion method, using 0.1 M imidazole (pH 8.0), 0.25 M Ca(OAc)2, and 15% PEG 3350 as a precipitant Xanthomonas campestris

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.17 E48A mutant shows only marginal activities of approximately 10% toward D-glucono-1,5-lactone compared to that of the wild type enzyme Xanthomonas campestris
3.1.1.17 N134A mutant shows only marginal activities of approximately 10% toward D-glucono-1,5-lactone compared to that of the wild type enzyme Xanthomonas campestris
3.1.1.17 N191 mutant shows only marginal activities of approximately 10% toward D-glucono-1,5-lactone compared to that of the wild type enzyme Xanthomonas campestris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.17 0.0162
-
 D-glucono-1,5-lactone in 1 mM potassium phosphate buffer (pH 7.0) with 0.075 mM CaCl2 Xanthomonas campestris

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.17 Ca2+ contains two calcium ions Xanthomonas campestris

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.17 Xanthomonas campestris B0RN69
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.17
-
 Xanthomonas campestris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.17 D-glucono-1,5-lactone + H2O XC5397 exhibits lactonase activity only toward D-glucono-1,5-lactone Xanthomonas campestris D-gluconate
-
 ?
3.1.1.17 additional information XC5397 does not hydrolyze D-gulono-1,4-lactone, L-gulono-1,4-lactone, and D-ribono-1,4-lactone Xanthomonas campestris ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.17 homodimer x-ray crystallography Xanthomonas campestris

Synonyms

EC Number Synonyms Comment Organism
3.1.1.17 GNL
-
 Xanthomonas campestris
3.1.1.17 SMP-30
-
 Xanthomonas campestris
3.1.1.17 XC5397
-
 Xanthomonas campestris