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Literature summary extracted from
- Overexpression, crystallization, and preliminary X-ray crystallographic analysis of the alanine racemase from Enterococcus faecalis v583 (2008), J. Microbiol. Biotechnol., 18, 55-58.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.1.1.1 | amplified from genomic DNA from Enterococcus faecalis via PCR, integrated into pET22b, transformation of Escherichia coli BL21 (DE3) for overexpression as His-tagged protein | Enterococcus faecalis |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 5.1.1.1 | hanging-drop vapor-diffusion method, protein concentration: 20 mg/ml; crystal screen buffer: 0.1 M HEPES, pH 8.0, 22% (w/v) PEG 8000, 0.3 M Ca-acetate, and 1/10 (v/v) cyclohexyl-methyl-beta-D-maltoside, drop: ratio 1/2 mixed protein solution and buffer (sum 2 microl) hanging over 1 ml of buffer; cryo-protecting solution: 0.1 M HEPES, pH 8.0, 22% (w/v) PEG 8000, 0.3 M Ca-acetate, 30% (v/v) glycerol and 1/10 (v/v) cyclohexyl-methyl-beta-D-maltoside for flashcooling in liquid nitrogen, diffracted to 2.5 A | Enterococcus faecalis |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.1.1.1 | 40100 | - |
SDS-PAGE | Enterococcus faecalis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.1.1.1 | L-alanine | Enterococcus faecalis | - |
D-alanine | - |
r |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.1.1.1 | Enterococcus faecalis | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.1.1.1 | Ni2+-NTA resin (His-tagged overexpressed protein), Hitrap Q-column, Superdex S prep grad column | Enterococcus faecalis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.1.1.1 | L-alanine | - |
Enterococcus faecalis | D-alanine | - |
r | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.1.1.1 | pyridoxal 5'-phosphate | - |
Enterococcus faecalis | |
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