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Literature summary extracted from

  • Lubertozzi, D.; Keasling, J.D.
    Expression of a synthetic Artemesia annua amorphadiene synthase in Aspergillus nidulans yields altered product distribution (2008), J. Ind. Microbiol. Biotechnol., 35, 1191-1198.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.2.3.24 pharmacology amorpha-4,11-diene is a precursor of artemisinin, an important agent in the treatment of malaria, produced via oxidation Artemisia annua

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.24 expression of a synthetic Artemesia annua amorphadiene synthase gene under control of a strong constitutive promoter ((p)gpdA) in Aspergillus nidulans yields altered product distribution. The transformants produces only small amounts of amorphadiene, but much larger amounts of similar sesquiterpenes normally produced as minor by-products in plants. Expression of ADS in Escherichia coli produces almost exclusively amorpha-4,11-diene. The host environment can greatly impact the terpenes produced from terpene synthases. Artemisia annua

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.24 2-trans,6-trans-farnesyl diphosphate Artemisia annua
-
 amorpha-4,11-diene + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.24 Artemisia annua
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.24 cell culture
-
 Artemisia annua
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.24 2-trans,6-trans-farnesyl diphosphate
-
 Artemisia annua amorpha-4,11-diene + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.3.24 ADS
-
 Artemisia annua
4.2.3.24 amorpha-4,11-diene synthase
-
 Artemisia annua
4.2.3.24 amorphadiene synthase
-
 Artemisia annua