EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
6.2.1.3 | additional information | the enzyme is inducible by wounding | Arabidopsis thaliana |
EC Number | Cloned (Comment) | Organism |
---|---|---|
6.2.1.12 | expressed in Escherichia coli strains DH5alpha and M15 | Arabidopsis thaliana |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
6.2.1.3 | additional information | construction of opcl1 mutant, At1g20510-KO-1, plants, which show no induction of jasmonate by wounding in contrast to the wild-type plants, jasmonate profiles in leaves, overview | Arabidopsis thaliana |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.2.1.3 | additional information | - |
additional information | Michaelis-Menten kinetics | Arabidopsis thaliana | |
6.2.1.B3 | 0.019 | - |
3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-octanoic acid | pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
6.2.1.B3 | 0.027 | - |
12-oxo-phytodienoic acid | pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
6.2.1.B3 | 0.036 | - |
tetradecanoic acid | pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
6.2.1.B3 | 0.076 | - |
dinor-12-oxo-phytodienoic acid | pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
6.2.1.B3 | 0.094 | - |
3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-hexanoic acid | pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
6.2.1.3 | additional information | subcellular localization study | Arabidopsis thaliana | - |
- |
6.2.1.3 | peroxisome | - |
Arabidopsis thaliana | 5777 | - |
6.2.1.B3 | peroxisome | - |
Arabidopsis thaliana | 5777 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
6.2.1.3 | Mg2+ | - |
Arabidopsis thaliana |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.2.1.3 | additional information | Arabidopsis thaliana | the OPC-8:CoA ligase catalyses an essential step in jasmonic acid biosynthesis by initiating the beta-oxidative chain shortening of the carboxylic acid side chain of its precursors, and, in accordance with this function, the protein is localized in peroxisomes | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.2.1.3 | Arabidopsis thaliana | - |
Col-0, Ws-0, gene OPCL1 | - |
6.2.1.B3 | Arabidopsis thaliana | Q84P21 | - |
- |
6.2.1.12 | Arabidopsis thaliana | - |
ecotype Col-0 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.2.1.12 | - |
Arabidopsis thaliana |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
6.2.1.3 | leaf | expression levels of OPCL1 | Arabidopsis thaliana | - |
6.2.1.12 | leaf | - |
Arabidopsis thaliana | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.2.1.B3 | 12-oxo-phytodienoic acid + CoA + ATP | - |
Arabidopsis thaliana | 12-oxo-phytodienoyl-CoA + AMP + diphosphate | - |
? | |
6.2.1.B3 | 3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-hexanoic acid + CoA + ATP | i.e. OPC-6:0 | Arabidopsis thaliana | 3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-hexanoyl-CoA + AMP + diphosphate | - |
? | |
6.2.1.B3 | 3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-octanoic acid + CoA + ATP | i.e. OPC-8:0 | Arabidopsis thaliana | 3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-octanoyl-CoA + AMP + diphosphate | - |
? | |
6.2.1.3 | ATP + 12-oxo-phytodienoic acid + CoA | - |
Arabidopsis thaliana | AMP + diphosphate + 12-oxo-phytodienoyl-CoA | - |
? | |
6.2.1.3 | ATP + 3-oxo-2(2'-[Z]-pentenyl)cyclopentane-1-hexanoic acid + CoA | - |
Arabidopsis thaliana | AMP + diphosphate + 3-oxo-2(2'-[Z]-pentenyl)cyclopentane-1-hexanoyl-CoA | - |
? | |
6.2.1.3 | ATP + 3-oxo-2(2'-[Z]-pentenyl)cyclopentane-1-octanoic acid + CoA | preferred substrate | Arabidopsis thaliana | AMP + diphosphate + 3-oxo-2(2'-[Z]-pentenyl)cyclopentane-1-octanoyl-CoA | - |
? | |
6.2.1.3 | ATP + dinor-12-oxo-phytodienoic acid + CoA | - |
Arabidopsis thaliana | AMP + diphosphate + dinor-12-oxo-phytodienoyl-CoA | - |
? | |
6.2.1.3 | ATP + jasmonic acid + CoA | - |
Arabidopsis thaliana | AMP + diphosphate + jasmonyl-CoA | - |
? | |
6.2.1.3 | ATP + myristic acid + CoA | - |
Arabidopsis thaliana | AMP + diphosphate + myristoyl-CoA | - |
? | |
6.2.1.B3 | decanoic acid + CoA + ATP | - |
Arabidopsis thaliana | decanoyl-CoA + AMP + diphosphate | - |
? | |
6.2.1.B3 | dinor-12-oxo-phytodienoic acid + CoA + ATP | - |
Arabidopsis thaliana | dinor-12-oxo-phytodienoyl-CoA + AMP + diphosphate | - |
? | |
6.2.1.B3 | dodecanoic acid + CoA + ATP | - |
Arabidopsis thaliana | dodecanoyl-CoA + AMP + diphosphate | - |
? | |
6.2.1.B3 | hexadecanoic acid + CoA + ATP | - |
Arabidopsis thaliana | hexadecanoyl-CoA + AMP + diphosphate | - |
? | |
6.2.1.3 | additional information | the OPC-8:CoA ligase catalyses an essential step in jasmonic acid biosynthesis by initiating the beta-oxidative chain shortening of the carboxylic acid side chain of its precursors, and, in accordance with this function, the protein is localized in peroxisomes | Arabidopsis thaliana | ? | - |
? | |
6.2.1.3 | additional information | enzyme family members display activity towards different biosynthetic precursors of jasmonic acid, including 12-oxo-phytodienoic acid, i.e. OPDA, dinor-OPDA, 3-oxo-2(2'-[Z]-pentenyl)cyclopentane-1-octanoic acid, i.e. OPC-8, and OPC-6, substrate specificity of protein At1g20510 readily converting OPDA, dnOPDA, OPC-8, OPC-6, and myristic acid, OPC-8 is the preferred substrate, overview | Arabidopsis thaliana | ? | - |
? | |
6.2.1.B3 | octadecanoic acid + CoA + ATP | - |
Arabidopsis thaliana | octadecanoyl-CoA + AMP + diphosphate | - |
? | |
6.2.1.B3 | oleic acid + CoA + ATP | - |
Arabidopsis thaliana | oleoyl-CoA + AMP + diphosphate | - |
? | |
6.2.1.B3 | tetradecanoic acid + CoA + ATP | - |
Arabidopsis thaliana | tetradecanoyl-CoA + AMP + diphosphate | - |
? | |
6.2.1.12 | AMP + 3-oxo-2-(2'-[Z]-pentenyl)-cyclopentane-1-butanoic acid + CoA | weak substrate | Arabidopsis thaliana | ? | - |
? | |
6.2.1.12 | AMP + 3-oxo-2-(2'-[Z]-pentenyl)-cyclopentane-1-hexanoic acid + CoA | weak substrate | Arabidopsis thaliana | ? | - |
? | |
6.2.1.12 | AMP + dinor-12-oxo-phytodienoic acid + CoA | - |
Arabidopsis thaliana | ? | - |
? | |
6.2.1.12 | ATP + 2-coumarate + CoA | - |
Arabidopsis thaliana | AMP + diphosphate + 2-coumaroyl-CoA | - |
? | |
6.2.1.12 | ATP + 3-coumarate + CoA | high activity | Arabidopsis thaliana | AMP + diphosphate + 3-coumaroyl-CoA | - |
? | |
6.2.1.12 | ATP + 4-coumarate + CoA | high activity | Arabidopsis thaliana | AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
6.2.1.12 | ATP + acetate + CoA | - |
Arabidopsis thaliana | AMP + diphosphate + acetyl-CoA | - |
? | |
6.2.1.12 | ATP + caffeate + CoA | high activity | Arabidopsis thaliana | AMP + diphosphate + caffeoyl-CoA | - |
? | |
6.2.1.12 | ATP + cinnamate + CoA | - |
Arabidopsis thaliana | AMP + diphosphate + cinnamoyl-CoA | - |
? | |
6.2.1.12 | ATP + decanoate + CoA | weak substrate | Arabidopsis thaliana | AMP + diphosphate + decanoyl-CoA | - |
? | |
6.2.1.12 | ATP + ferulate + CoA | - |
Arabidopsis thaliana | AMP + diphosphate + feruloyl-CoA | - |
? | |
6.2.1.12 | ATP + heptanoate + CoA | - |
Arabidopsis thaliana | AMP + diphosphate + heptanoyl-CoA | - |
? | |
6.2.1.12 | ATP + hexadecanoate + CoA | weak substrate | Arabidopsis thaliana | AMP + diphosphate + hexadecanoyl-CoA | - |
? | |
6.2.1.12 | ATP + hexanoate + CoA | - |
Arabidopsis thaliana | AMP + diphosphate + hexanoyl-CoA | - |
? | |
6.2.1.12 | ATP + indole propanoic acid + CoA | - |
Arabidopsis thaliana | ? | - |
? | |
6.2.1.12 | ATP + nonanoate + CoA | - |
Arabidopsis thaliana | AMP + diphosphate + nonanoyl-CoA | - |
? | |
6.2.1.12 | ATP + octadecanoate + CoA | weak substrate | Arabidopsis thaliana | AMP + diphosphate + octadecanoyl-CoA | - |
? | |
6.2.1.12 | ATP + octanoate + CoA | - |
Arabidopsis thaliana | AMP + diphosphate + octanoyl-CoA | - |
? | |
6.2.1.12 | ATP + oleate + CoA | - |
Arabidopsis thaliana | AMP + diphosphate + oleoyl-CoA | - |
? | |
6.2.1.12 | ATP + propanoate + CoA | weak substrate | Arabidopsis thaliana | AMP + diphosphate + propanoyl-CoA | - |
? | |
6.2.1.12 | ATP + tetradecanoate + CoA | - |
Arabidopsis thaliana | AMP + diphosphate + tetradecanoyl-CoA | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.2.1.3 | fatty acyl-CoA synthetase | - |
Arabidopsis thaliana |
6.2.1.3 | More | the enzyme belongs to a fatty acyl-CoA synthetase family, which is distinct from the known long-chain fatty acyl-CoA synthetase, LACS, and the 4-coumarate:CoA ligase, 4CL, families, overview | Arabidopsis thaliana |
6.2.1.3 | OPC-8:CoA ligase | - |
Arabidopsis thaliana |
6.2.1.3 | OPCL1 | - |
Arabidopsis thaliana |
6.2.1.3 | protein At1g20510 | - |
Arabidopsis thaliana |
6.2.1.12 | 4-coumarate:CoA ligase | - |
Arabidopsis thaliana |
6.2.1.12 | 4CL2 | - |
Arabidopsis thaliana |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.2.1.B3 | 0.96 | - |
tetradecanoic acid | pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
6.2.1.B3 | 1.27 | - |
3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-octanoic acid | pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
6.2.1.B3 | 1.49 | - |
3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-hexanoic acid | pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
6.2.1.B3 | 1.56 | - |
12-oxo-phytodienoic acid | pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
6.2.1.B3 | 1.78 | - |
dinor-12-oxo-phytodienoic acid | pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
6.2.1.3 | ATP | - |
Arabidopsis thaliana | |
6.2.1.12 | ATP | - |
Arabidopsis thaliana |
EC Number | Organism | Comment | Expression |
---|---|---|---|
6.2.1.B3 | Arabidopsis thaliana | expression is activated in response to wounding or infection | up |
EC Number | General Information | Comment | Organism |
---|---|---|---|
6.2.1.B3 | physiological function | mutants exhibit 3-oxo-2(2'-[Z]-pentenyl)cyclopentane-1-octanoic acid accumulation and reduced levels of jasmonic acid. Enzyme catalyzes an essential step in jasmonic acid biosynthesis by initiating the beta-oxidative chain shortening of the carboxylic acid side chain of its precursors | Arabidopsis thaliana |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.2.1.B3 | 15.2 | - |
3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-hexanoic acid | pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
6.2.1.B3 | 23.4 | - |
dinor-12-oxo-phytodienoic acid | pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
6.2.1.B3 | 26.4 | - |
tetradecanoic acid | pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
6.2.1.B3 | 56.2 | - |
12-oxo-phytodienoic acid | pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
6.2.1.B3 | 65.4 | - |
3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-octanoic acid | pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana |