EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.9.4 | in pKGWFS7 for expression N-terminally of an (eGFP)-beta-glucoronidase fusion and Agrobacterium-mediated transformation of Arabidopsis plants, in pK7FWG2 (as full-length or 158 N-terminal amino acid fragment) for expression as eGFP-fusion and subcellular localization in onion epidermis and Arabidopsis thaliana, in pPS48uYFP as full-length, C-terminal truncations (N-terminal 610, 158, or 61 amino acids) or C-terminally fused to transit peptide of AtGWD isoform 1 for Agrobacterium-mediated expression in Nicotiana benthamiana, in pGEM-4Z (as full-length or as fusion with transit peptide of AtGWD1) for in vitro transcription, translation and chloroplast import assays | Arabidopsis thaliana |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.7.9.4 | cell wall | along the cell wall, N-terminal 158 and 61 amino acids (revealed by confocal laser scanning microscopy) | Arabidopsis thaliana | 5618 | - |
2.7.9.4 | additional information | extra-plastidial localisation of full-length enzyme (in vitro chloroplast import assay), lack of chloroplast transit peptide | Arabidopsis thaliana | - |
- |
2.7.9.4 | additional information | no fluorescence signal detected for full-length enzyme or its 610 N-terminal residues fused to eYFP or eGFP | Arabidopsis thaliana | - |
- |
2.7.9.4 | nucleus | N-terminal 158 and 61 amino acids (revealed by confocal laser scanning microscopy) | Arabidopsis thaliana | 5634 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.9.4 | ATP + alpha-glucan + H2O | Arabidopsis thaliana | exclusive phosphorylation of C-6 position, in vitro | AMP + phospho-alpha-glucan + phosphate | - |
? | |
2.7.9.4 | additional information | Arabidopsis thaliana | not involved in transient starch degradation, plants lacking AtGWD show similar starch and sugar content as wild-type plants | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.9.4 | Arabidopsis thaliana | Q9STV0 | ecotype Columbia-0, wild-type or mutant lacking AtGWD2 | - |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
2.7.9.4 | phosphoprotein | autophosphorylation | Arabidopsis thaliana |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.9.4 | by metal-affinity chromatography after expression in Saccharomyces cerevisiae with C-terminal V5- and hexa-His-tag | Arabidopsis thaliana |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.7.9.4 | abscission zone | floral organ, strong expression in vascular tissue just before onset of senescence (promoter activity) | Arabidopsis thaliana | - |
2.7.9.4 | cauline leaf | high expression level (microarray) | Arabidopsis thaliana | - |
2.7.9.4 | companion cell | phloem, high expression just before onset of senescence (promoter activity) | Arabidopsis thaliana | - |
2.7.9.4 | flower | strong expression in mature flower parts (microarray) | Arabidopsis thaliana | - |
2.7.9.4 | leaf | strong expression in vascular tissue (abaxial side of primary vascular bundle, phloem) just before onset of senescence (promoter activity), senescent leaves (microarray) | Arabidopsis thaliana | - |
2.7.9.4 | additional information | spatially (vascular) and temporarily (age-dependent) restricted expression pattern (promoter activity) | Arabidopsis thaliana | - |
2.7.9.4 | root | strong expression in vascular tissue just before onset of senescence (promoter activity) | Arabidopsis thaliana | - |
2.7.9.4 | sepal | strong expression in vascular tissue just before onset of senescence (promoter activity) | Arabidopsis thaliana | - |
2.7.9.4 | silique | strong expression in vascular tissue (floral organ abscission zone, dehiscence zone between silique walls, vascular tissue of silique walls) just before onset of senescence (promoter activity, microarray) | Arabidopsis thaliana | - |
2.7.9.4 | stamen | strong expression in vascular tissue just before onset of senescence (promoter activity) | Arabidopsis thaliana | - |
2.7.9.4 | stem | strong expression in vascular tissue just before onset of senescence (promoter activity) | Arabidopsis thaliana | - |
2.7.9.4 | vascular tissue | leaf, stem, root, flower, silique only just before onset of senescence (promoter activity) | Arabidopsis thaliana | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.9.4 | 0.02 | - |
amylopectin, mean chain length: 23.5, nmol glucose-6-phosphate/mg: 0.1 | Arabidopsis thaliana |
2.7.9.4 | 0.04 | - |
amylopectin, pre-phosphorylated, mean chain length: 24.8, nmol glucose-6-phosphate/mg: 0.5 | Arabidopsis thaliana |
2.7.9.4 | 0.49 | - |
amylopectin, elongated, mean chain length: 29.7, nmol glucose-6-phosphate/mg: 0.1 | Arabidopsis thaliana |
2.7.9.4 | 0.65 | - |
amylopectin, elongated and pre-phosphorylated, mean chain length: 30.8, nmol glucose-6-phosphate/mg: 39.4 | Arabidopsis thaliana |
2.7.9.4 | 1.36 | - |
soluble potato starch | Arabidopsis thaliana |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.9.4 | ATP + alpha-glucan + H2O | exclusive phosphorylation of C-6 position, in vitro | Arabidopsis thaliana | AMP + phospho-alpha-glucan + phosphate | - |
? | |
2.7.9.4 | ATP + amylopectin + H2O | 1 h, 30°C, pH 7, in presence of 10 mM NH4Cl, 10 mM MgCl2, 0.5 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, substrates: waxy maize amylopectin (unmodified (glycogen), elongated by phosphorylase a, pre-phosphorylated by potato glucan, water dikinase (GWD), or elongated and pre-phosphorylated) or soluble potato starch | Arabidopsis thaliana | AMP + phospho-amylopectin + phosphate | reaction stop by boiling | ? | |
2.7.9.4 | additional information | not involved in transient starch degradation, plants lacking AtGWD show similar starch and sugar content as wild-type plants | Arabidopsis thaliana | ? | - |
? | |
2.7.9.4 | additional information | activity is independent of pre-phosphorylation of glucan substrate | Arabidopsis thaliana | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.9.4 | alpha-glucan, water dikinase | - |
Arabidopsis thaliana |
2.7.9.4 | AtGWD2 | At4g24450 | Arabidopsis thaliana |