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Literature summary extracted from

  • Ma, Q.H.
    Characterization of a cinnamoyl-CoA reductase that is associated with stem development in wheat (2007), J. Exp. Bot., 58, 2011-2021.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.44 additional information CCR enzyme activity is increased progressively along with lignin biosynthesis and stem maturity. During stem development, CCR1 mRNA levels remain high at elongation, heading, and milky stages in the wheat H4564 cultivar. High CCR1 mRNA levels and high CCR enzyme activity in wheat stem are correlated with a higher Klason lignin content and greater stem mechanical strength in the H4564 cultivar. Escherichia coli BL21(DE3) and BL21(DE3)pLysS strains produce a much higher amount of CCR1 protein compared with the RIL and RP strains Triticum aestivum

Application

EC Number Application Comment Organism
1.2.1.44 additional information CCR1 is present as a single-copy gene in the wheat genome. It groups together with other monocot CCR sequences while it diverges from CCR2. It may be involved in the regulation of lignin biosynthesis during stem maturity and may contribute to stem strength support in wheat Triticum aestivum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.44 cloned into the pET-29b vector and expressed in Escherichia coli strains BL21(DE3), BL21(DE3)pLysS, RIL, and RP Triticum aestivum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.44 additional information CCR enzyme activity declines dramatically at the heading and milky stages in stems of the C6001 cultivar Triticum aestivum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.44 0.01437
-
 caffeoyl-CoA
-
 Triticum aestivum
1.2.1.44 0.06006
-
 sinapoyl-CoA
-
 Triticum aestivum
1.2.1.44 0.07697
-
 feruloyl-CoA
-
 Triticum aestivum
1.2.1.44 0.1824
-
 5-hydroxyferuloyl-CoA
-
 Triticum aestivum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.44 43000
-
 x * 43000, SDS-PAGE Triticum aestivum

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.44 Triticum aestivum A8DNN6 H4564 cultivar and C6001 cultivar
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.44 by gel filtration, to homogeneity Triticum aestivum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.44 leaf lower expression Triticum aestivum
-
1.2.1.44 additional information undetectable expression in roots Triticum aestivum
-
1.2.1.44 stem highly expressed Triticum aestivum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.44 5-hydroxyferuloyl-CoA + NADPH + H+
-
 Triticum aestivum 5-hydroxyferulic aldehyde + CoA + NADP+
-
 ?
1.2.1.44 caffeoyl-CoA + NADPH + H+
-
 Triticum aestivum caffealdehyde + CoA + NADP+
-
 ?
1.2.1.44 feruloyl-CoA + NADPH + H+ best substrate, which indicates preferential biosynthesis of G-type lignin Triticum aestivum coniferaldehyde + CoA + NADP+
-
 ?
1.2.1.44 sinapoyl-CoA + NADPH + H+
-
 Triticum aestivum sinapaldehyde + CoA + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.44 ? x * 43000, SDS-PAGE Triticum aestivum

Synonyms

EC Number Synonyms Comment Organism
1.2.1.44 CCR1
-
 Triticum aestivum