BRENDA - Enzyme Database

Saposin B-dependent reconstitution of arylsulfatase a activity in vitro and in cell culture models of metachromatic leukodystrophy

Matzner, U.; Breiden, B.; Schwarzmann, G.; Yaghootfam, A.; Fluharty, A.L.; Hasilik, A.; Sandhoff, K.; Gieselmann, V.; J. Biol. Chem. 284, 9372-9381 (2009)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.6.8
bis(monoacylglycero) phosphate
at a relative concentration of 30 mol% the lipid stimulates the reaction rate by a factor of 3.4
Homo sapiens
3.1.6.8
dolichol
at a relative concentration of 30 mol% the lipid stimulates the reaction rate by a factor of 2.7
Homo sapiens
3.1.6.8
additional information
phosphatidylinositol and phosphatidylserine have no effect on the rate of sulfatide hydrolysis
Homo sapiens
3.1.6.8
phosphatidic acid
at a relative concentration of 30 mol% the lipid stimulates the reaction rate by a factor of 3.1
Homo sapiens
Cloned(Commentary)
EC Number
Commentary
Organism
3.1.6.8
expressed in baby hamster kidney cells and CHO cells
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.6.8
Ca2+
CaCl2 reduces the reaction rate in the low millimolar range
Homo sapiens
3.1.6.8
Mg2+
MgCl2 reduces the reaction rate in the low millimolar range
Homo sapiens
3.1.6.8
Mn2+
MnCl2 reduces the reaction rate in the low millimolar range
Homo sapiens
3.1.6.8
Na+
enzymatic activity is inhibited at NaCl concentrations exceeding 20 mM, at 50 and 200 mM the rate of sulfatide hydrolysis is diminished by about 50 and 75%, respectively
Homo sapiens
3.1.6.8
phosphate
competitive inhibitor
Homo sapiens
3.1.6.8
sulfite
competitive inhibitor
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.6.8
lysosome
-
Homo sapiens
5764
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.6.8
Homo sapiens
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.6.8
4-nitrocatechol sulfate + H2O
-
693260
Homo sapiens
4-nitrocatechol + sulfate
-
-
-
?
3.1.6.8
4-nitrophenyl sulfate + H2O
-
693260
Homo sapiens
4-nitrophenol + sulfate
-
-
-
?
3.1.6.8
cerebroside 3-sulfate + H2O
the reaction requires saposin B, a non-enzymatic proteinaceous cofactor which presents sulfatide to the catalytic site of arylsulfatase A, desulfation is negligible when Sap B is substituted by Sap A, C, or D
693260
Homo sapiens
cerebroside + sulfate
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.6.8
4.3
-
narrow pH optimum around pH 4.3
Homo sapiens
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.6.8
3.75
4.75
desulfation activity is less than half-maximum at pH values below pH 3.75 and above pH 4.75
Homo sapiens
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.6.8
bis(monoacylglycero) phosphate
at a relative concentration of 30 mol% the lipid stimulates the reaction rate by a factor of 3.4
Homo sapiens
3.1.6.8
dolichol
at a relative concentration of 30 mol% the lipid stimulates the reaction rate by a factor of 2.7
Homo sapiens
3.1.6.8
additional information
phosphatidylinositol and phosphatidylserine have no effect on the rate of sulfatide hydrolysis
Homo sapiens
3.1.6.8
phosphatidic acid
at a relative concentration of 30 mol% the lipid stimulates the reaction rate by a factor of 3.1
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.6.8
expressed in baby hamster kidney cells and CHO cells
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.6.8
Ca2+
CaCl2 reduces the reaction rate in the low millimolar range
Homo sapiens
3.1.6.8
Mg2+
MgCl2 reduces the reaction rate in the low millimolar range
Homo sapiens
3.1.6.8
Mn2+
MnCl2 reduces the reaction rate in the low millimolar range
Homo sapiens
3.1.6.8
Na+
enzymatic activity is inhibited at NaCl concentrations exceeding 20 mM, at 50 and 200 mM the rate of sulfatide hydrolysis is diminished by about 50 and 75%, respectively
Homo sapiens
3.1.6.8
phosphate
competitive inhibitor
Homo sapiens
3.1.6.8
sulfite
competitive inhibitor
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.6.8
lysosome
-
Homo sapiens
5764
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.6.8
4-nitrocatechol sulfate + H2O
-
693260
Homo sapiens
4-nitrocatechol + sulfate
-
-
-
?
3.1.6.8
4-nitrophenyl sulfate + H2O
-
693260
Homo sapiens
4-nitrophenol + sulfate
-
-
-
?
3.1.6.8
cerebroside 3-sulfate + H2O
the reaction requires saposin B, a non-enzymatic proteinaceous cofactor which presents sulfatide to the catalytic site of arylsulfatase A, desulfation is negligible when Sap B is substituted by Sap A, C, or D
693260
Homo sapiens
cerebroside + sulfate
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.6.8
4.3
-
narrow pH optimum around pH 4.3
Homo sapiens
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.6.8
3.75
4.75
desulfation activity is less than half-maximum at pH values below pH 3.75 and above pH 4.75
Homo sapiens