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Literature summary extracted from

  • Matzner, U.; Breiden, B.; Schwarzmann, G.; Yaghootfam, A.; Fluharty, A.L.; Hasilik, A.; Sandhoff, K.; Gieselmann, V.
    Saposin B-dependent reconstitution of arylsulfatase a activity in vitro and in cell culture models of metachromatic leukodystrophy (2009), J. Biol. Chem., 284, 9372-9381.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.6.8 bis(monoacylglycero) phosphate at a relative concentration of 30 mol% the lipid stimulates the reaction rate by a factor of 3.4 Homo sapiens
3.1.6.8 dolichol at a relative concentration of 30 mol% the lipid stimulates the reaction rate by a factor of 2.7 Homo sapiens
3.1.6.8 additional information phosphatidylinositol and phosphatidylserine have no effect on the rate of sulfatide hydrolysis Homo sapiens
3.1.6.8 phosphatidic acid at a relative concentration of 30 mol% the lipid stimulates the reaction rate by a factor of 3.1 Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.6.8 expressed in baby hamster kidney cells and CHO cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.6.8 Ca2+ CaCl2 reduces the reaction rate in the low millimolar range Homo sapiens
3.1.6.8 Mg2+ MgCl2 reduces the reaction rate in the low millimolar range Homo sapiens
3.1.6.8 Mn2+ MnCl2 reduces the reaction rate in the low millimolar range Homo sapiens
3.1.6.8 Na+ enzymatic activity is inhibited at NaCl concentrations exceeding 20 mM, at 50 and 200 mM the rate of sulfatide hydrolysis is diminished by about 50 and 75%, respectively Homo sapiens
3.1.6.8 phosphate competitive inhibitor Homo sapiens
3.1.6.8 sulfite competitive inhibitor Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.6.8 lysosome
-
 Homo sapiens 5764
-

Organism

EC Number Organism UniProt Comment Textmining
3.1.6.8 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.6.8 4-nitrocatechol sulfate + H2O
-
 Homo sapiens 4-nitrocatechol + sulfate
-
 ?
3.1.6.8 4-nitrophenyl sulfate + H2O
-
 Homo sapiens 4-nitrophenol + sulfate
-
 ?
3.1.6.8 cerebroside 3-sulfate + H2O the reaction requires saposin B, a non-enzymatic proteinaceous cofactor which presents sulfatide to the catalytic site of arylsulfatase A, desulfation is negligible when Sap B is substituted by Sap A, C, or D Homo sapiens cerebroside + sulfate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.6.8 arylsulfatase A
-
 Homo sapiens
3.1.6.8 ASA
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.6.8 4.3
-
 narrow pH optimum around pH 4.3 Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.6.8 3.75 4.75 desulfation activity is less than half-maximum at pH values below pH 3.75 and above pH 4.75 Homo sapiens