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Literature summary extracted from

  • Lai, L.; Xu, Z.; Zhou, J.; Lee, K.D.; Amidon, G.L.
    Molecular basis of prodrug activation by human valacyclovirase, an alpha-amino acid ester hydrolase (2008), J. Biol. Chem., 283, 9318-9327.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.1.1.43 drug development valacyclovirase is suggested as a prodrug activating enzyme Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.43 into the vector pET17b for expression in Escherichia coli BL21DE3 and B834DE3 cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.1.43 the crystal structures of the native protein, the S122A and D123N mutants, and the native enzyme in complex with L-tyrosine amide are solved at resolutions of 1.75 A, 1.90 A, 1.85 A and 1.90 A, respectively Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.43 D123N point mutant Homo sapiens
3.1.1.43 S122A point mutant Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.43 Mg2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.43 valacyclovir + H2O Homo sapiens
-
 L-valine + acyclovir
-
 ?
3.1.1.43 valganciclovir + H2O Homo sapiens
-
 L-valine + ganciclovir
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.43 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.43 using a S strong cation exchange and a phenyl-Sepharose hydrophobic interaction column Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.43 0.6
-
 3-amino-3-phenylpropionic acid ethyl ester Homo sapiens
3.1.1.43 1.8
-
 beta-Ala benzyl ester Homo sapiens
3.1.1.43 35.3
-
 L-Trp benzyl ester Homo sapiens
3.1.1.43 41.4
-
 L-Tyr benzyl ester Homo sapiens
3.1.1.43 75.3
-
 L-Phe ethyl ester Homo sapiens
3.1.1.43 103.7
-
 valacyclovir Homo sapiens
3.1.1.43 156.2
-
 L-Met methyl ester Homo sapiens
3.1.1.43 156.3
-
 L-Val benzyl ester Homo sapiens
3.1.1.43 358.3
-
 L-Phe benzyl ester Homo sapiens
3.1.1.43 484.3
-
 L-Ser benzyl ester Homo sapiens
3.1.1.43 635
-
 L-Leu benzyl ester Homo sapiens
3.1.1.43 849.4
-
 L-Gly benzyl ester Homo sapiens
3.1.1.43 1368
-
 L-Ala benzyl ester Homo sapiens
3.1.1.43 5148
-
 L-Pro benzyl ester Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.43 3-amino-3-phenylpropionic acid ethyl ester + H2O
-
 Homo sapiens ?
-
 ?
3.1.1.43 beta-Ala benzyl ester + H2O
-
 Homo sapiens beta-Ala + benzoic acid
-
 ?
3.1.1.43 floxuridine + H2O
-
 Homo sapiens ?
-
 ?
3.1.1.43 gemcitabine + H2O
-
 Homo sapiens ?
-
 ?
3.1.1.43 Gly benzyl ester + H2O
-
 Homo sapiens Gly + benzoic acid
-
 ?
3.1.1.43 L-Ala benzyl ester + H2O
-
 Homo sapiens L-Ala + benzoic acid
-
 ?
3.1.1.43 L-Leu benzyl ester + H2O
-
 Homo sapiens L-leu + benzoic acid
-
 ?
3.1.1.43 L-Met methyl ester + H2O
-
 Homo sapiens ?
-
 ?
3.1.1.43 L-Phe benzyl ester + H2O
-
 Homo sapiens L-Phe + benzoic acid
-
 ?
3.1.1.43 L-Phe ethyl ester + H2O
-
 Homo sapiens ?
-
 ?
3.1.1.43 L-Pro benzyl ester + H2O
-
 Homo sapiens L-Pro + benzoic acid
-
 ?
3.1.1.43 L-Ser benzyl ester + H2O
-
 Homo sapiens L-Ser + benzoic acid
-
 ?
3.1.1.43 L-Trp benzyl ester + H2O
-
 Homo sapiens L-Trp + benzoic acid
-
 ?
3.1.1.43 L-Tyr benzyl ester + H2O
-
 Homo sapiens L-Tyr + benzoic acid
-
 ?
3.1.1.43 L-Val benzyl ester + H2O
-
 Homo sapiens L-Val + benzoic acid
-
 ?
3.1.1.43 valacyclovir + H2O
-
 Homo sapiens L-valine + acyclovir
-
 ?
3.1.1.43 valganciclovir + H2O
-
 Homo sapiens L-valine + ganciclovir
-
 ?
3.1.1.43 zidovudine + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.43 alpha-amino acid ester esterase
-
 Homo sapiens
3.1.1.43 alpha-amino acid ester hydrolase
-
 Homo sapiens
3.1.1.43 VACVase
-
 Homo sapiens
3.1.1.43 valacyclovirase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.43 37
-
 activity assay Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.43 7.4
-
 activity assay Homo sapiens