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Literature summary extracted from

  • Cao, C.; Lu, S.; Kivlin, R.; Wallin, B.; Card, E.; Bagdasarian, A.; Tamakloe, T.; Chu, W.M.; Guan, K.L.; Wan, Y.
    AMP-activated protein kinase contributes to UV- and H2O2-induced apoptosis in human skin keratinocytes (2008), J. Biol. Chem., 283, 28897-28908.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.31 5-aminoimidazole-4-carboxamide-1-beta-D-ribonucleoside i.e. AICAR Homo sapiens
2.7.11.31 AMP
-
 Homo sapiens
2.7.11.31 additional information UV and H2O2 induce AMPK activation through Thr172 phosphorylation, UV and H2O2 also phosphorylate LKB1, an upstream signal of AMPK, in an epidermal growth factor receptor-dependent manner, overview. SB203580, a p38 inhibitor, does not affect AMPK activation Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.31 additional information AMPK-specific small interfering RNA knockdown Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.31 compound C i.e. AMPKi or 6-[4-(2-piperidin-1-yl-ethoxy)-phenyl]-3-pyridin-4-yl-pyrrazolo[1,5-a]-pyrimidine, a specific inhibitor of AMPK, largely impairs the activation of p38 MAPK upon UV radiation Homo sapiens
2.7.11.31 additional information activation of p38 in response to UV or H2O2 is inhibited in AMPKalpha siRNA-treated HaCaT cells, EGFR inhibitor PD 153035 and AG 1478 inhibit UV-induced AMPK and LKB1 activation Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.31 Mg2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.31 ATP + p38 Homo sapiens phosphorylation at Thr180/Thr182, p38 MAPK is a downstream signal of AMPK upon various stimuli, AMPK serves as a positive regulator for p38 Ser15 phosphorylation induced by UV radiation and H2O2 treatment ADP + phospho-p38
-
 ?
2.7.11.31 ATP + p53 Homo sapiens AMPK serves as a positive regulator for p38 Ser15 phosphorylation induced by UV radiation and H2O2 treatment ADP + phospho-p53
-
 ?
2.7.11.31 ATP + PFK2 Homo sapiens phosphorylation at Ser466 induced by UV radiation and H2O2 treatment ADP + phospho-PFK2
-
 ?
2.7.11.31 ATP + [acetyl-CoA carboxylase] Homo sapiens phosphorylation at Ser79 ADP + phospho-[acetyl-CoA carboxylase]
-
 ?
2.7.11.31 additional information Homo sapiens AMP-activated protein kinase contributes to UV- and H2O2-induced apoptosis in human skin keratinocytes, AMPK serves as a negative feedback signal against UV-induced mammalian target of rapamycin, mTOR activation in a TSC2-dependent manner, AMPK plays important roles in UV-induced signal transduction ultimately leading to skin photoaging and even skin cancer, regulation, overview ?
-
 ?
6.4.1.2 additional information Homo sapiens regulation of ACC is necessary for maintaining the energy balance ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.31 Homo sapiens
-
-
-
6.4.1.2 Homo sapiens
-
 isozymes ACC1 and ACC2
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.11.31 phosphoprotein UV and H2O2 induce AMPK activation through Thr172 phosphorylation Homo sapiens
6.4.1.2 phosphoprotein ACC is phosphorylated at Ser79 and thereby inactivated by AMP-activated protein kinase, AMPK, which is induced by UV light and H2O2. AMPK phosphorylates both isozymes of ACC, ACC1 and ACC2 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.31 cell culture
-
 Homo sapiens
-
2.7.11.31 HaCaT cell
-
 Homo sapiens
-
2.7.11.31 keratinocyte
-
 Homo sapiens
-
2.7.11.31 skin fibroblast
-
 Homo sapiens
-
6.4.1.2 HaCaT cell
-
 Homo sapiens
-
6.4.1.2 keratinocyte
-
 Homo sapiens
-
6.4.1.2 skin
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.31 additional information
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.31 ATP + p38 phosphorylation at Thr180/Thr182, p38 MAPK is a downstream signal of AMPK upon various stimuli, AMPK serves as a positive regulator for p38 Ser15 phosphorylation induced by UV radiation and H2O2 treatment Homo sapiens ADP + phospho-p38
-
 ?
2.7.11.31 ATP + p38 phosphorylation at Thr180/Thr182 Homo sapiens ADP + phospho-p38
-
 ?
2.7.11.31 ATP + p53
-
 Homo sapiens ADP + phospho-p53
-
 ?
2.7.11.31 ATP + p53 AMPK serves as a positive regulator for p38 Ser15 phosphorylation induced by UV radiation and H2O2 treatment Homo sapiens ADP + phospho-p53
-
 ?
2.7.11.31 ATP + PFK2 phosphorylation at Ser466 induced by UV radiation and H2O2 treatment Homo sapiens ADP + phospho-PFK2
-
 ?
2.7.11.31 ATP + [acetyl-CoA carboxylase] phosphorylation at Ser79 Homo sapiens ADP + phospho-[acetyl-CoA carboxylase]
-
 ?
2.7.11.31 additional information AMP-activated protein kinase contributes to UV- and H2O2-induced apoptosis in human skin keratinocytes, AMPK serves as a negative feedback signal against UV-induced mammalian target of rapamycin, mTOR activation in a TSC2-dependent manner, AMPK plays important roles in UV-induced signal transduction ultimately leading to skin photoaging and even skin cancer, regulation, overview Homo sapiens ?
-
 ?
6.4.1.2 additional information regulation of ACC is necessary for maintaining the energy balance Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.31 AMP-activated protein kinase
-
 Homo sapiens
6.4.1.2 ACC
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.31 ATP
-
 Homo sapiens