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Literature summary extracted from

  • Borodina, I.; Siebring, J.; Zhang, J.; Smith, C.P.; van Keulen, G.; Dijkhuizen, L.; Nielsen, J.
    Antibiotic overproduction in Streptomyces coelicolor A3 2 mediated by phosphofructokinase deletion (2008), J. Biol. Chem., 283, 25186-25199.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.7.1.11 biotechnology design of strains with improved antibiotic production Streptomyces coelicolor

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.11 expressed in Escherichia coli Streptomyces coelicolor

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.11 additional information PFKA1-deletion strain, constructed by replacement of a chromosomal sequence within an Streptomyces coelicolor cosmid Streptomyces coelicolor
2.7.1.11 additional information PFKA2-deletion strain, constructed by replacement of a chromosomal sequence in a Streptomyces coelicolor cosmid, 2-6fold increased antibiotic production, accumulation of glucose 6-phosphate, changes of intracellular fluxes, no enzyme activity Streptomyces coelicolor
2.7.1.11 additional information PFKA3-deletion strain, constructed by replacement of a chromosomal sequence in a Streptomyces coelicolor cosmid Streptomyces coelicolor

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.11 ATP + D-fructose 6-phosphate Streptomyces coelicolor
-
 ADP + D-fructose 1,6-bisphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.11 Streptomyces coelicolor O08333 A3(2), strain M145
-
2.7.1.11 Streptomyces coelicolor Q9FC99 pfkA3 gene; A3(2), strain M145
-
2.7.1.11 Streptomyces coelicolor Q9L1L8 A3(2), strain M145
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.11 gel filtration Streptomyces coelicolor

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.11 additional information
-
 characterization of the PFKA2-deletion strain by metabolic flux analysis and transcription analysis, transcriptional changes in regulatory and membrane proteins as well as in fatty acid metabolism, carbon flux through the pentose phosphate pathway increased, PFKA2 protein involved in determining the carbon flux distribution, production of the pigmented antibiotics actinorhodin and undecylprodigiosin up to 6fold increased in the deletion strain Streptomyces coelicolor
2.7.1.11 additional information
-
 integrated analysis of gene expression data by genome-scale metabolic model simulations, deletion mutant strain produces antibiotics in quantities comparable with that of the reference strain, enzyme activities like in wild-type attributed to intact copy of pfkA2 gene Streptomyces coelicolor
2.7.1.11 additional information
-
 integrated analysis of gene expression data by genome-scale metabolic model simulations, deletion mutant strain produces antibiotics in quantities comparable with that of the reference strain, wild type-like phosphofructokinase activities measured, attributed to intact copy of pfkA2 gene Streptomyces coelicolor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.11 ATP + D-fructose 6-phosphate
-
 Streptomyces coelicolor ADP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 ATP + D-fructose 6-phosphate engineering to increase antibiotic production Streptomyces coelicolor ADP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 ATP + D-fructose 6-phosphate improved antibiotic production by deletion of PFKA2, involvement of PFKA2 in determining the carbon flux distribution Streptomyces coelicolor ADP + D-fructose 1,6-bisphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.11 PFKA1
-
 Streptomyces coelicolor
2.7.1.11 PFKA2
-
 Streptomyces coelicolor
2.7.1.11 PFKA3
-
 Streptomyces coelicolor