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Literature summary extracted from

  • Hu, T.; Wu, D.; Chen, J.; Ding, J.; Jiang, H.; Shen, X.
    The catalytic intermediate stabilized by a "down" active site loop for diaminopimelate decarboxylase from Helicobacter pylori. Enzymatic characterization with crystal structure analysis (2008), J. Biol. Chem., 283, 21284-21293.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.20 expressed in Escherichia coli strain BL21 (DE3) Helicobacter pylori

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.1.20 hanging drop vapour diffusion method, in 100 mM bicine, pH 8.0, and 1.6 M ammonium sulfate, at 4°C Helicobacter pylori

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.20 I148A mutant shows 2% catalytic efficiency compared to the wild type enzyme Helicobacter pylori
4.1.1.20 I148D mutant shows 0.2% catalytic efficiency compared to the wild type enzyme Helicobacter pylori
4.1.1.20 I148F mutant shows 15% catalytic efficiency compared to the wild type enzyme Helicobacter pylori
4.1.1.20 I148G mutant shows 0.5% catalytic efficiency compared to the wild type enzyme Helicobacter pylori
4.1.1.20 I148K mutant shows 2% catalytic efficiency compared to the wild type enzyme Helicobacter pylori
4.1.1.20 I148L mutant shows 28% catalytic efficiency compared to the wild type enzyme Helicobacter pylori

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.20 0.03
-
 meso-2,6-diaminoheptanedioate mutant enzyme I148L, in 100 mM Tris, pH 8.0 Helicobacter pylori
4.1.1.20 0.11
-
 meso-2,6-diaminoheptanedioate mutant enzyme I148F, in 100 mM Tris, pH 8.0 Helicobacter pylori
4.1.1.20 0.35
-
 meso-2,6-diaminoheptanedioate mutant enzyme I148A, in 100 mM Tris, pH 8.0 Helicobacter pylori
4.1.1.20 0.36
-
 meso-2,6-diaminoheptanedioate mutant enzyme I148G, in 100 mM Tris, pH 8.0 Helicobacter pylori
4.1.1.20 0.36
-
 meso-2,6-diaminoheptanedioate mutant enzyme I148K, in 100 mM Tris, pH 8.0 Helicobacter pylori
4.1.1.20 0.39
-
 meso-2,6-diaminoheptanedioate wild type enzyme, in 100 mM Tris, pH 8.0 Helicobacter pylori
4.1.1.20 0.72
-
 meso-2,6-diaminoheptanedioate mutant enzyme I148D, in 100 mM Tris, pH 8.0 Helicobacter pylori

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.20 Helicobacter pylori P56129
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.20 Ni-NTA resin column chromatography Helicobacter pylori

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.20 meso-2,6-Diaminoheptanedioate
-
 Helicobacter pylori L-Lysine + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.1.20 DAPDC
-
 Helicobacter pylori
4.1.1.20 meso-diaminopimelate decarboxylase
-
 Helicobacter pylori

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.20 0.07
-
 meso-2,6-diaminoheptanedioate mutant enzyme I148D, in 100 mM Tris, pH 8.0 Helicobacter pylori
4.1.1.20 0.07
-
 meso-2,6-diaminoheptanedioate mutant enzyme I148G, in 100 mM Tris, pH 8.0 Helicobacter pylori
4.1.1.20 0.28
-
 meso-2,6-diaminoheptanedioate mutant enzyme I148K, in 100 mM Tris, pH 8.0 Helicobacter pylori
4.1.1.20 0.33
-
 meso-2,6-diaminoheptanedioate mutant enzyme I148A, in 100 mM Tris, pH 8.0 Helicobacter pylori
4.1.1.20 0.38
-
 meso-2,6-diaminoheptanedioate mutant enzyme I148L, in 100 mM Tris, pH 8.0 Helicobacter pylori
4.1.1.20 0.68
-
 meso-2,6-diaminoheptanedioate mutant enzyme I148F, in 100 mM Tris, pH 8.0 Helicobacter pylori
4.1.1.20 15.65
-
 meso-2,6-diaminoheptanedioate wild type enzyme, in 100 mM Tris, pH 8.0 Helicobacter pylori

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.20 pyridoxal 5'-phosphate
-
 Helicobacter pylori