EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.7.69 | - |
Arabidopsis thaliana |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.7.69 | G224D | less than 0.1% of wild-type activity | Arabidopsis thaliana |
2.7.7.69 | H238N | less than 0.1% of wild-type activity | Arabidopsis thaliana |
2.7.7.69 | S290F | less than 0.1% of wild-type activity | Arabidopsis thaliana |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.69 | 0.0044 | - |
GDP-alpha-D-glucose | pH 7.5, 26°C | Arabidopsis thaliana | |
2.7.7.69 | 0.0079 | - |
GDP-alpha-L-galactose | pH 7.5, 26°C | Arabidopsis thaliana | |
2.7.7.69 | 0.0083 | - |
GDP-alpha-L-galactose | pH 7.5, 26°C | Arabidopsis thaliana | |
2.7.7.69 | 0.012 | - |
GDP-alpha-D-glucose | pH 7.5, 26°C | Arabidopsis thaliana | |
2.7.7.69 | 0.22 | - |
phosphate | presence of GDP-G-glucose, pH 7.5, 26°C | Arabidopsis thaliana | |
2.7.7.69 | 0.52 | - |
GDP-alpha-D-mannose | pH 7.5, 26°C | Arabidopsis thaliana | |
2.7.7.69 | 0.76 | - |
phosphate | presence of GDP-alpha-D-glucose, pH 7.5, 26°C | Arabidopsis thaliana | |
2.7.7.69 | 1 | - |
phosphate | presence of GDP-L-galactose, pH 7.5, 26°C | Arabidopsis thaliana | |
2.7.7.69 | 1.3 | - |
GDP-alpha-D-mannose | pH 7.5, 26°C | Arabidopsis thaliana | |
2.7.7.69 | 2.4 | - |
phosphate | presence of GDP-alpha-L-galactose, pH 7.5, 26°C | Arabidopsis thaliana | |
2.7.7.69 | 6 | - |
alpha-L-galactose 1-phosphate | pH 7.5, 26°C | Arabidopsis thaliana | |
2.7.7.69 | 10 | - |
alpha-D-glucose 1-phosphate | pH 7.5, 26°C | Arabidopsis thaliana | |
2.7.7.69 | 16 | - |
alpha-D-galactose 1-phosphate | pH 7.5, 26°C | Arabidopsis thaliana | |
2.7.7.69 | 29 | - |
alpha-D-glucose 1-phosphate | pH 7.5, 26°C | Arabidopsis thaliana | |
2.7.7.69 | 45 | - |
alpha-D-galactose 1-phosphate | pH 7.5, 26°C | Arabidopsis thaliana |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.69 | GDP-beta-L-galactose + phosphate | Arabidopsis thaliana | enzyme guanylylates a conserved active site His residue with GDP-L-galactose, forming L-galactose 1-phosphate for vitamin C synthesis, and regeneration of the enzyme with phosphate to form GDP | GDP + beta-L-galactose 1-phosphate | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.7.69 | Arabidopsis thaliana | Q8RWE8 | isoform Vtc2 | - |
2.7.7.69 | Arabidopsis thaliana | Q9FLP9 | isoform Vtc5 | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.7.69 | 14.2 | - |
pH 7.5, 26°C, substrate GDP-alpha-D-glucose | Arabidopsis thaliana |
2.7.7.69 | 15.1 | - |
pH 7.5, 26°C, substrate GDP-alpha-L-galactose | Arabidopsis thaliana |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.69 | alpha-D-glucose 1-phosphate + GDP | - |
Arabidopsis thaliana | GDP-alpha-D-glucose + phosphate | - |
r | |
2.7.7.69 | alpha-D-mannose 1-phosphate + GDP | - |
Arabidopsis thaliana | GDP-alpha-D-mannose + phosphate | - |
r | |
2.7.7.69 | alpha-L-galactose 1-phosphate + GDP | - |
Arabidopsis thaliana | GDP-alpha-L-galactose + phosphate | - |
r | |
2.7.7.69 | GDP-alpha-D-galactose + phosphate | - |
Arabidopsis thaliana | GDP + alpha-D-galactose 1-phosphate | - |
r | |
2.7.7.69 | GDP-alpha-D-glucose + phosphate | - |
Arabidopsis thaliana | alpha-D-glucose 1-phosphate + GDP | - |
r | |
2.7.7.69 | GDP-alpha-D-mannose + phosphate | - |
Arabidopsis thaliana | alpha-D-mannose 1-phosphate + GDP | - |
r | |
2.7.7.69 | GDP-beta-L-galactose + phosphate | enzyme guanylylates a conserved active site His residue with GDP-L-galactose, forming L-galactose 1-phosphate for vitamin C synthesis, and regeneration of the enzyme with phosphate to form GDP | Arabidopsis thaliana | GDP + beta-L-galactose 1-phosphate | - |
r | |
2.7.7.69 | GDP-beta-L-galactose + phosphate | reaction proceeds through a covalent guanylated histidine residue within the histidine triad motif | Arabidopsis thaliana | GDP + beta-L-galactose 1-phosphate | - |
r | |
2.7.7.69 | additional information | poor reactivity of hexose 1-phosphates as acceptors | Arabidopsis thaliana | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.7.69 | Vtc5 | - |
Arabidopsis thaliana |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.69 | 0.024 | - |
GDP-alpha-D-mannose | pH 7.5, 26°C | Arabidopsis thaliana | |
2.7.7.69 | 0.093 | - |
GDP-alpha-D-mannose | pH 7.5, 26°C | Arabidopsis thaliana | |
2.7.7.69 | 3.8 | - |
alpha-D-galactose 1-phosphate | pH 7.5, 26°C | Arabidopsis thaliana | |
2.7.7.69 | 5.8 | - |
alpha-D-glucose 1-phosphate | pH 7.5, 26°C | Arabidopsis thaliana | |
2.7.7.69 | 6 | - |
alpha-D-galactose 1-phosphate | pH 7.5, 26°C | Arabidopsis thaliana | |
2.7.7.69 | 6 | - |
alpha-L-galactose 1-phosphate | pH 7.5, 26°C | Arabidopsis thaliana | |
2.7.7.69 | 9.9 | - |
GDP-alpha-D-glucose | pH 7.5, 26°C | Arabidopsis thaliana | |
2.7.7.69 | 12 | - |
alpha-D-glucose 1-phosphate | pH 7.5, 26°C | Arabidopsis thaliana | |
2.7.7.69 | 13 | - |
GDP-alpha-L-galactose | pH 7.5, 26°C | Arabidopsis thaliana | |
2.7.7.69 | 23 | - |
GDP-alpha-D-glucose | pH 7.5, 26°C | Arabidopsis thaliana | |
2.7.7.69 | 27 | - |
GDP-alpha-L-galactose | pH 7.5, 26°C | Arabidopsis thaliana |